When deconvolving 2-photon data what microscope type should we choose?

Very large or no pinhole

If there is a non-descanned detector (NDD), or a very large (say > 10 Airy disks) pinhole, the contribution of the pinhole to the image formation can be ignored. In this case the microscope type can be widefield in combination with 2p photon. This will result in more realistic values for the Nyquist rate than a confocal setting.

Fairly large pinhole

If there is a pinhole in the order of a couple of Airy disks, typically 500nm backprojected radius, it must be taken into account. So in this case the microscope type type should be set to confocal.

Keywords: 2-photon pinhole NDD
Categories: Faq Microscopy, Huygens Faq,Imported Faqs
Platforms: Irix Linux Mac
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