How large a resolution improvement can be expected from the Huygens deconvolution?
As a rule of thumb one gets easily 2x in Z and a bit less in XY. This applies to even noisy images. To gain more resolution a measured PSF is necessary.
Some published figures (Bioimaging 4 1996 pp. 187-197): HIW (nm) z x y
- raw bead image 790 270 265
- restored 221 116 93
- bead object function 83 83 83 (i.e. the `true' bead)
- difference 138 33 10
Conclusion: nearly 4x in Z, more than 2x in XY.
Keywords: resolution HIW
Categories: Faq Deconvolution, Faq Microscopy, Huygens Faq, Imported Faqs
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