Confocal image showing basal bodies of the cilia in a paramecium. Image was provided by A. Aubusson-Fleury, CNRS, Gif sur Yvette, Paris.
All Microscope Brands
All brands of single point-scanning Confocal Microscopes are supported, as well as many File Formats.
Reach 120nm Resolution
120nm resolution is achievable with Huygens.
Use advanced Maximum Likelihood Estimation algorithms. Learn more: Huygens Deconvolution Algorithms.
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Dr. Christina Schlatterer, SFB969/LS Deuerling, University of Konstanz, Germany.
"Researchers who use Huygens at the facility to enhance their confocal data are very much impressed by the results. I would not have anticipated myself how much especially noisy confocal data can be improved by deconvolution with Huygens. As noisy data is the rule rather than the exception with live samples, we now use Huygens routinely to enhance our spinning disk data."
Dr. Ulrike Engel, Scientific Director of the NIC Imaging Centre, Bioquant, University of Heidelberg, Germany.
Image of DAPI (Red) and mab22C10 (Green) staining in larval brain of Drosophila melanogaster. The image was captured using Leica SP5II confocal microscope. Image was kindly provided by Dr. Anand Krishna Tiwari, Indian Institute of Advanced Studies, India.
Yeast organelle transport. Time series acquisition, Single point-scanning Confocal. Late-Golgi marker Sec7-DsRed, Early Golgi marker Cog1-GFP. Perfectly acquired, but noisy confocal time series of low abundance proteins in yeast endosomal and Golgi system allows accurate object analysis after deconvolution with Huygens. Image kindly provided by Prof. Benjamin S. Glick, MGCB, University of Chicago, United States.
Image shows the remarkable complexity of the actin filament network in primary mouse embryonic fibroblasts (pMEF) null for the actin regulatory proteins Tm5NM1/2. Actin filaments are visualised by phalloidin. Confocal image (N.A1.4. 100x) was deconvolved and visualised with Huygens Professional. Image kindly provided by Mr. Howard Vindin. Cellular & Genetic Medicine Unit, School of Medical Sciences, University of New South Wales, Australia.
A series of developing Drosophila oocytes (egg-chambers to be precise). A Z-stack of 116 optical sections was taken on a Leica SP8 Laser scanning confocal microscope with a 63x 1.4NA objective. Actin (in yellow) is stained with rhodamin phalloidin and the nuclear envelopes of the so -called nurse cells are labeled with autofluorescence of GFP-Tm1 (green). HRM was used to deconcolve the raw image. Image kindly provided by Dr. Imre Gaspar, Developmental Biology Unit, EMBL Heidelberg, Germany.
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