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STED Deconvolution Image Gallery


Image Description
o14_Series_website_image3.jpg STED deconvolution resolves better detail. Pre-synaptic marker (Atto647n on 2nd antibody) within the neuromuscular junction of Drosophila melanogaster larvae shows holes in flower structures that were deconvolved with Huygens (picture in the middle), and that can not be identified in the raw Leica STED image (left). Raw pulsed-STED image was first stabilized and then deconvolved with Huygens.

Image used with permission from Oliver Kobler, Ulrich Thomas and Werner Zuschratter, CNI, Leibniz Institute Magdeburg, Germany

Before_After_STED_Deconv.png STED deconvolution of silica rods. The raw CW-STED image of fluorescent colloidal silica rods was deconvolved with Huygens. The image is significantly improved with Huygens STED deconvolution.

Image used with permission from Thijs Besseling, Department of Physics and Astronomy, Soft Condensed Matter and Biophysics, Utrecht University, The Netherlands

Series022_gl_and_Series022_gl_deconvCMLE_stable.jpg Huygens STED deconvolution clearly improves lateral resolution. Single XY plane of STED data before (left) and after stabilization and deconvolution (right) of the complete z-stack with Huygens. Structure (240 nm ring diameter) was stained with primary and secondary antibody conjugated to Alexa488.

Data used by permission from Dr. Grazvydas Lukinavicius, EPFL, Lausanne, Switzerland

mipXZ_Series022_gl_raw_deconvolved.jpg Axial MIP projection of the complete STED z-stack shows the axial improvement with STED deconvolution. Axial MIP projection of the complete z-stack of pulsed-STED data before (left) and after stabilization and deconvolution (right) with Huygens. Structure (240 nm ring diameter) was stained with primary and secondary antibody conjugated to Alexa488, and images with a Leica STED system.

Data used by permission from Dr. Grazvydas Lukinavicius, EPFL, Lausanne, Switzerland

Figure.png Huygens deconvolution of a pulsed-STED image of fixed neuronal cells. Leica pulsed-STED image taken from a neuronal cell-culture model (differentiated SH-SY5Y cells). Cells were fixed and stained with an anti-tubulin primary antibody and anti-rabbit-Atto647N secondary antibody. Inset shows a magnification of one specific area.

Data used with permission of Dr. Heinz-Georg Jahnke/Prof. Dr. Andrea A. Robitzki, Molecular biological-biochemical Processing Technology, Biotechnological-Biomedical Center, Deutscher Platz 5, 04103 Leipzig, Germany. Funded by the Free State of Saxony and the European Union (SMWK/EFRE)

APD__Figure.png Confocal and STED image of chromosomes with histon proteins before and after deconvolution. Combined image of chromosomes (confocal in green) and histon protein (STED in red) image before and after deconvolution. More contrast and resolution can be observed after deconvolution of both the Leica confocal and STED channel. The image below shows an enlargement of the red channel.

Data obtained from Dr. Juraj Kabat, Biological Imaging Facility, NIH/NIAID, Bethesda, USA

APD__Confocal_STED_Deconv_RedChannel.png Confocal and STED image of histon proteins. Confocal and Leica pulsed-STED (red) image before and after deconvolution. The image is an enlargement of the red channel (Histon proteins) shown in the image above.

Data obtained from Dr. Juraj Kabat, Biological Imaging Facility, NIH/NIAID, Bethesda, USA

SCP3_Figure.png Chromosomes separated by STED and deconvolution. Both the raw Leica STED image and after subsequent Huygens deconvolution show the two tangled X chromosomes separately at bottom, whereas they can not be separated by regular confocal microscopy.

Data obtained from Dr. Juraj Kabat, Biological Imaging Facility, NIH/NIAID, Bethesda, USA

Series049_Confocal_STED_Raw_and_decon_Figure.png Confocal and STED image of primary neurons before and after deconvolution. Confocal and CW (non-gated) STED (Leica SP8; 100% STED power) images of primary astrocytes before and after deconvolution with Huygens. Sample was stained for cytochrome C (Alexa-488).

Data obtained from Dr. Asier Ruiz Nunez, Achucarro Basque Center for Neuroscience and Department of Neurosciences,. University of Basque Country (UPV/EHU), Leioa, Spain


More images can be seen at the Image Gallery.

More deconvolution and visualization examples can also be found on the Restoration Examples page in our support wiki.

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