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Huygens Image Contest 2015


It is with great pleasure that we announce this years winners of the Huygens Image Contest. But first, we want to thank all of you for submitting outstanding high quality images and movies.

After carefully reviewing the images, we decided to grant the first prize, which is a Sony Alpha A5000 system camera to Dr. Pawel Pasierbek from the BioOptics Facility (IMP-IMBA-GMI), Vienna, Austria.

He submitted an image of tissue cultured neurons acquired with a spinning disk confocal microscope and deconvolved with Huygens professional. The image is displayed below and will cover our 2015 Christmas card.
The next five prize winners are also shown below. They all win a Google Chromecast (Audio or Video).

1st Prize: Dr. Pawel Pasierbek, BioOptics Facility of the IMP-IMBA-GMI, Vienna, Austria
2nd Prize: Dr. Matyas Molnar, BioVis Facility, Uppsala University, Sweden
3rd Prize: Dr. Helfrid Hochegger, Genome and Stability Centre, University of Sussex, UK
4th Prize: Dr. Arndt Meyer, Animal Navigation Group, University of Oldenburg, Germany
5th Prize: Dr. Jorge Bernardino de la Serna, Science and Technology Facilities, Rutherford Appleton Laboratory, UK
6th Prize: Dr. Jing Yan, Department of Molecular Biology, Princeton University, USA

The complete SVI team congratulates the winners and thanks all the participants for making this Huygens Image Contest into a great success.

We wish you all nice holidays and a happy 2016, and we hope you will join the next years Huygens Image Contest.

The SVI team

1st Prize

Image from Pawel Pasierbek, BioOptics (IMP, IMBA, GMI), Vienna, Austria.
Download the full size image here

Spinning disk confocal image of tissue culture neiurons deconvolved with Huygens professional.
Spinning disk confocal image of tissue culture neiurons deconvolved with Huygens professional.


2nd Prize

Image from Matyas Molnar from the BioVis imaging facility, Uppsala University, Sweden.
Download the full size image here

Ant head autofluorescence captured with a Zeiss 710 confocal microscope using 488nm laser excitation and detection of two channes (blue and green). The acquired 3D dataset was deconvolved and maximum intensity projection image was rendered with Huygens.
Ant head autofluorescence captured with a Zeiss 710 confocal microscope using 488nm laser excitation and detection of two channes (blue and green). The acquired 3D dataset was deconvolved and maximum intensity projection image was rendered with Huygens.


3rd Prize

Image submitted by Helfrid Hochegger, Genome and Stability Centre, Sussex Universty, UK.
Download the full size image here

The image shows glioma stem cells grown on matrigel and stained by IF
with tubulin (green, actin (red), centromeres/CREST (white) and DAPI (blue). The image was taken on an Olympus IX73 using a 40x 0.9NA.
The image shows glioma stem cells grown on matrigel and stained by IF with tubulin (green, actin (red), centromeres/CREST (white) and DAPI (blue). The image was taken on an Olympus IX73 using a 40x 0.9NA.


4th Prize

Image submitted by Arndt Meyer, Animal Navigation Group, University of Oldenburg, Germany.
Download the full size image here

Calbindin immunoreactivity in the ganglion cell layer of a mouse retina. The image was taken close to the center of the retina, where the ganglion cell axons gather in thick bundles and then leave the eye as the optic nerve. This confocal stack was recorded on a Leica SP8 microscope, deconvolved using Huygens Essential, and finally color-coded for depth.
Calbindin immunoreactivity in the ganglion cell layer of a mouse retina. The image was taken close to the center of the retina, where the ganglion cell axons gather in thick bundles and then leave the eye as the optic nerve. This confocal stack was recorded on a Leica SP8 microscope, deconvolved using Huygens Essential, and finally color-coded for depth.


5th Prize

Image submitted by Jorge Bernardino Rutherford Appleton Laboratory, UK.
Download the full size image here

Image of a T lymphocyte crawling taken with a Leica SP8 g-STED.
The image is deconvolved with Huygens and the final visual effect was obtained using the Huygens surface rendering tool.
Blue shows labelled plasma membrane with a specific lipophilic dye, red represents the actin filaments, and yellow internal cytosolic vesicles.
Image of a T lymphocyte crawling taken with a Leica SP8 g-STED. The image is deconvolved with Huygens and the final visual effect was obtained using the Huygens surface rendering tool. Blue shows labelled plasma membrane with a specific lipophilic dye, red represents the actin filaments, and yellow internal cytosolic vesicles.


6th Prize

Image submitted by Jing Yan, Princeton University, USA.
Download the full size image here

Image shows a growing community of bacteria Vibrio cholerae, the pathogen for cholerae. Bacteria adopt a social life form known as biofilm, where they stay together and secrete chemicals to build a
Image shows a growing community of bacteria Vibrio cholerae, the pathogen for cholerae. Bacteria adopt a social life form known as biofilm, where they stay together and secrete chemicals to build a "home" for survival. This Nikon spinning disc confocal image was deconvolved with a theoretical PSF in Huygens and finally color coded for depth.

Contact Information

Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands


Phone: +31 (0)35 64216 26
Fax: +31 (0)35 683 7971
E-mail: info at svi.nl

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