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Winners of the Huygens Image Contest 2016


With great pleasure, we announce this years winners of the Huygens Image Contest. Yet first, we want to thank all of you for submitting such outstanding high quality images and movies.

After a thorough review of the images, we decided to distinguish two different categories of images, namely "Cells" and "Tissues and Organisms", each with a first prize winner. The first prize for the "Cells" goes to a gorgeous image from Dr. Daniela Malide from the Light Microscopy Core Facility of the NIH - National Heart, Blood and Lung Institute (Bethesda, USA). For the category "Tissues and Organisms", we selected one of the two beautiful images sent by Outi Paloheimo from the BioMediTech - Imaging Facility, University of Tampere, Finland. We congratulate both Dr. Malide and Mrs. Paloheimo with winning an ASUS Chromebook.
The next three prize winners are also shown below. They all win a Google Chromecast (Audio or Video).

1st Prize: Dr. Daniela Malide, Light Microscopy Core Facility of the NIH - National Heart, Blood and Lung Institute, Bethesda, USA.
1st Prize: Mrs. Outi Paloheimo from the Neuro Group BioMediTech, and Imaging Facility, University of Tampere, Finland.
3rd Prize: Dr. Priyam Banerjee, MD Anderson Cancer Center, Houston, Texas, USA.
4th Prize: Mrs. Julia Sauerwald, Group of Prof. Stefan Luschnig, Institute for Neurobiology, WWU Muenster, Germany.
5th Prize: Dr. Eva Wegel, JIC BioImaging, John Innes Centre, Norwich, United Kingdom.

We congratulate all the winners and thank all the participants for making this Huygens Image Contest into a great success.
We wish you all nice holidays and a happy 2017, and we hope you will join the next years Huygens Image Contest (again).

The SVI team

1st Prize Winners

Image in the category "Cells", from Daniela Malide

COS-7 (fibroblast-like) cell line with mitochondria in green/white and cytoskeletal protein tubulin in red/magenta. Comparing the diffraction limited confocal image (green/red) with the super-resolution STED (Stimulated Emission Depletion) microscopy image (white/magenta). STED reveals thin tubulin strands (magenta) and details of the TOM-20 stained outer mitochondria membrane (white). Images were taken with a Leica SP8 STED 3X microscope and deconvolved with Huygens software.
COS-7 (fibroblast-like) cell line with mitochondria in green/white and cytoskeletal protein tubulin in red/magenta. Comparing the diffraction limited confocal image (green/red) with the super-resolution STED (Stimulated Emission Depletion) microscopy image (white/magenta). STED reveals thin tubulin strands (magenta) and details of the TOM-20 stained outer mitochondria membrane (white). Images were taken with a Leica SP8 STED 3X microscope and deconvolved with Huygens software.


Image in the category "Tissue and Organisms", from Outi Paloheimo

The image represents a portion of the wing (showing arrangement of scales) of ''Pieris brassicae'' (cabbage butterfly). A Zeiss LSM 780 confocal microscope (25x/0.8 objective) with excitation lasers 488 nm, 535 nm, 633 nm was used to image the autofluorescence. The image was deconvolved using Huygens Essential (CMLE and theoretical PSF).
The image represents a portion of the wing (showing arrangement of scales) of ''Pieris brassicae'' (cabbage butterfly). A Zeiss LSM 780 confocal microscope (25x/0.8 objective) with excitation lasers 488 nm, 535 nm, 633 nm was used to image the autofluorescence. The image was deconvolved using Huygens Essential (CMLE and theoretical PSF).


3rd Prize

Image submitted by Priyam Banerjee

Human lung adenocarcinoma tissue with the extracellular matrix protein Collagen I immunostained in red (Alexa Fluor 568), collagen modifying enzyme Lysine Hydroxylase II (LH2) immunostained in green (Alexa Fluor 488). Nuclei were counterstained in blue (DAPI). Image was acquired with a Nikon A1+ confocal using Plan Apo 10X 0.45 NA objective. The image was deconvolved and SFP rendered with Huygens Professional version 16.10.
Human lung adenocarcinoma tissue with the extracellular matrix protein Collagen I immunostained in red (Alexa Fluor 568), collagen modifying enzyme Lysine Hydroxylase II (LH2) immunostained in green (Alexa Fluor 488). Nuclei were counterstained in blue (DAPI). Image was acquired with a Nikon A1+ confocal using Plan Apo 10X 0.45 NA objective. The image was deconvolved and SFP rendered with Huygens Professional version 16.10.


4th Prize

Image submitted by Julia Sauerwald

The image shows a Drosophila thorax captured with a Zeiss 880 confocal microscope and deconvolved using Huygens Essential. Respiratory tracheal branches are labeled in various colors using stochastic expression of spaghetti monster GFP with different epitope tags.
The image shows a Drosophila thorax captured with a Zeiss 880 confocal microscope and deconvolved using Huygens Essential. Respiratory tracheal branches are labeled in various colors using stochastic expression of spaghetti monster GFP with different epitope tags.


5th Prize

Image submitted by Eva Wegel

The image is a maximum intensity projection (MIP) of a z-stack through a ''Drosophila'' macrophage imaged on a Leica TCS SP8X scanning confocal microscope. Objective NA 1.4. Pinhole size 405nm 0.17 AU, 488nm 0.15 AU, 568nm 0.15 AU for obtaining the best possible resolution. 
Nuclei are DAPI stained (cyan), alpha-tubulin was detected with a primary mouse DM1A antibody and a secondary anti-mouse antibody coupled to Alexa Fluor 488 (magenta), actin was labelled with phalloidin coupled to Alexa Fluor 568 (green). Deconvolution was done with CMLE using Huygens Essential default settings.
The image is a maximum intensity projection (MIP) of a z-stack through a ''Drosophila'' macrophage imaged on a Leica TCS SP8X scanning confocal microscope. Objective NA 1.4. Pinhole size 405nm 0.17 AU, 488nm 0.15 AU, 568nm 0.15 AU for obtaining the best possible resolution. Nuclei are DAPI stained (cyan), alpha-tubulin was detected with a primary mouse DM1A antibody and a secondary anti-mouse antibody coupled to Alexa Fluor 488 (magenta), actin was labelled with phalloidin coupled to Alexa Fluor 568 (green). Deconvolution was done with CMLE using Huygens Essential default settings.