Zeiss LSM700

This is part of the Backprojected Pinhole Calculator.


The microscope reports the side length (s) of a square pinhole in microns (µm). Use this reported value in the form below.

Because of the square pinhole, the shape factor is $$c=1/\sqrt{\pi}\approx0.5642$$. The system magnification is reported to be 1.53. The simplified equation to calculate the Back Projected Pinhole Radius in nanometers is therefore $$r_b\approx 564 s / (1.53 m_{obj})$$.


Pinhole side (microns)
Objective magnification