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Zeiss LSM880


This is part of the Backprojected Pinhole Calculator.

Details


When using the LSM880 as a confocal, the microscope reports the side length (s) of a square pinhole in microns (µm). Use this reported value in the form below.

Because of the square pinhole, the shape factor is c=1/\sqrt{\pi}\approx0.5642. The system magnification is reported to be 1.9048. The simplified equation to calculate the Back Projected Pinhole Radius in nanometers is therefore r_b\approx 564 s / (1.9048 m_{obj}).

Calculator

Pinhole side (microns)
Objective magnification



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Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands


Phone: +31 (0)35 64216 26
Fax: +31 (0)35 683 7971
E-mail: info at svi.nl

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