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Zeiss LSM880


This is part of the Backprojected Pinhole Calculator.

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When using the LSM880 as a confocal, the microscope reports the side length (s) of a square pinhole in microns (µm). Use this reported value in the form below.

Because of the square pinhole, the shape factor is \(c=1/\sqrt{\pi}\approx0.5642\). The system magnification is reported to be 1.9048. The simplified equation to calculate the Back Projected Pinhole Radius in nanometers is therefore \(r_b\approx 564 s / (1.9048 m_{obj})\).

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Pinhole side (microns)
Objective magnification