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News

Huygens deconvolved image on cover Trends in Pharmacological Sciences.

Thursday 09 of August, 2012
A collaboration between the University of Pittsburgh School of Medicine, and the Massachusetts General Hospital and Harvard Medical School has led to a beautiful cover of the August issue of Trends in Pharmacological Sciences. The cover and accompanying paper, created by the authors J-P. Vilardaga, T.J. Gardella, V.L. Wehbi, and T.N. Feinstein, shows a Huygens deconvolved live early endosome. Click on this link to view the full cover.

Dr. Timothy Feinstein (Department of Pharmacology and Chemical Biology of the University of Pittsburgh School of Medicine, USA) provided us detailed information:
The image is of an early endosome labeled with PTHR-GFP (green), Vps29-YFP (blue) and beta-arrestin 1-dTomato (red), captured using a Nikon A1s spectral confocal at the Nyquist limit, based on the SVI Nyquist calculator, and deconvolved using Huygens Professional. The presentation is a reconstruction of the Huygens output that was alpha blended in Elements AR. Columns represent rotations of the endosome around the vertical axis and rows represent different combinations of color to show colocalization (red/green and blue/green) and lack of colocalization (red/blue).

See Trends in Pharmacological Sciences August issue for the paper.

Huygens 4.2 released with a new STED deconvolution option

Tuesday 12 of June, 2012
The SVI team has released Huygens version 4.2 with many new additions including a brand new STED deconvolution option.

Tests with the LEICA STED microscopes images deconvolved with Huygens STED deconvolution show a huge increase in contrast (~10 times), more resolution in Z, and a significant improvement in the already high lateral STED resolution, revealing spatial detail that can only be seen after deconvolution!

Huygens takes the specific STED parameters into consideration that are reported by the LEICA LIF files. But STED parameters can also be automatically estimated from bead images using the Huygens PSF distiller, which has been specifically extended to fullfil this task.

Batch wise deconvolution of STED images is also possible with the 4.2 Batch Processor.

In addition many other new functionalities have been added to the Huygens software that are available for customers with active licenses.

For example, both the Huygens Essential and Professional 4.2 include a completely new hot pixel removal tool that not only detects but also corrects for hot pixels. The deconvolution wizard has now image comparison tools integrated, and the Colocalization Analyzer is extended with smart background estimators.
We also added to all Huygens basics support of very large tiff files (larger than 4 GB); also known as BigTiffs.
These and all other new features can be viewed at here.

STED deconvolution now available in Huygens

Thursday 23 of February, 2012
Stimulated Emission Depletion (STED) microscopic images can now be deconvolved with the new Huygens STED option with truly stunning results. You can find the latest version at the Download page under development versions.

Developed bij Prof. Stefan Hell and now manufactured by Leica Microsystems, STED microscopes offer true super-resolution. The unique feature of the STED microscope is that it does not have a bandwidth limit, i.e. a barrier beyond which object details are not imaged anymore. While other super-resolution systems are still hampered by this wavelength dependent limit, a STED microscope moves happily beyond it.

Leica now offers two types of STED microscopes of which the CW version allows the usage of all kind of fluophores in the green range thus enabling full biological capabilities.

The Z-resolution, a very important part of biological research, is currently on the level of confocal Z-resolution. By applying Huygens STED deconvolution option you will get a huge increase in contrast (~10 times), more resolution in Z, and also improve the already high lateral STED resolution.

As always, Huygens deconvolution reduces noise and blurring, and takes depth-dependent spherical aberration into account. Geometrical distortions can also be easily corrected in Huygens.



Huygens Confocal and STED deconvolution

Example images obtained from Dr. Juraj Kabat (Biological Imaging Facility, NIH/NIAID, Bethesda, USA), who tested the Huygens STED deconvolution option and commented on the results: "I am really excited" and "it is clearly visible that the STED image is giving much more spatial details, although they are visible only after deconvolution".


Comparision of confocal and STED images, before and after deconvolution with Huygens.
Signal shows regulatory proteins Dmc1 and Rad51 for DNA repair during homologous chromosome recombination in meiosis. Dmc1 shown in green: Mega 520 - 532/685 for ex/em and 770 for depletion. Rad51 shown in red: Atto 647 640/685 ex/em and 770 for depletion. Courtesy of Dr. Juraj Kabat (Biological Imaging Facility, NIH/NIAID, Bethesda, USA.)
Comparision of confocal and STED images, before and after deconvolution with Huygens. Signal shows regulatory proteins Dmc1 and Rad51 for DNA repair during homologous chromosome recombination in meiosis. Dmc1 shown in green: Mega 520 - 532/685 for ex/em and 770 for depletion. Rad51 shown in red: Atto 647 640/685 ex/em and 770 for depletion. Courtesy of Dr. Juraj Kabat (Biological Imaging Facility, NIH/NIAID, Bethesda, USA.)



You are welcome to explore the new Huygens STED option using a free_trial.png version.