A true Nyquist rate does not exist for STED.
Instead, we calculate a sampling rate that is both practical and high enough to capture all information realistically available.

Acquisition depth is always 0 µm for 4Pi.

Calculate Calculating

Results

This is the parameter list used in this calculation:

For Confocal Microscope images recorded with pinholes
of around 1 Airy disk diameter,
lateral sampling distances may be up to 1.6 times of the recommended Nyquist values without significantly compromising image quality.
When small pinholes are used (< 0.5 Airy disk),
up to 1.3 times larger; with very large pinholes (> 4 Airy disks), up to 2 times.

xy MIP grayscalexy MIP false color xz MIP grayscalexz MIP false color
These are xy and xz
MIP projections of the 3D
point spread function.
The 3D image dimensions (x × y × z) are × × µm.
It was calculated using the Nyquist sample sizes specified above.
The number of samples (x × y × z) to cover this volume at the Nyquist rate is × × .
To enhance dimmer regions the gamma is corrected by 1.7 in the false color image.