Huygens version 4.3 is now released with many new improvements and a completely new optional Crosstalk Corrector.

A list of the most important improvements can be found on WhatsNew.

Crosstalk (also termed crossover or bleedthrough) can occur when acquiring a Multi Channel image. In that situation, the emission radiation of a given wavelength is detected by the wrong detector because part of the photons go through the wrong optical path inside the microscope (e.g. because the filters efficiency is not 100%). Therefore, some signal is actually recorded as coming from a certain dye when it really comes from a different one.

Crosstalk within Multi Channel images dramatically affects almost any type of data analysis, such as for example CoLocalization. In line with our aim to improve microscopic image quality and measurements, we offer from Huygens version 4.3 onwards the Crosstalk Corrector tool in the Huygens Software to correct for this imaging artifact.
More specific information of the Crosstalk Corrector can be found here

If you like to test the latest Huygens 4.3 version, you can get a of Huygens