For many years we already have a freely accessible online Nyquist Calculator on our website.

This Nyquist Calculator determines the optimal theoretical sampling density for your microscopic-image acquisition. With the computed sampling density you will make optimal use of the resolution of your microscope and minimize image distortions. Additionally, the Nyquist Calculator can visualize the Point Spread Function (PSF) for various microscope types and parameters in all dimensions.

Since January 2015 we have two versions:

• The Nyquist Calculator on the SVI website which is now expanded with STED and 3D STED, next to Widefield, Confocal, Spinning Disc, Multi-photon and Generic.
• The Nyquist Calculator App for Android which will be soon expanded to include also STED and 3D STED next to its current 5 modes. You can install it now from https://play.google.com/store/apps/details?id=com.svi.nyquistcalculator.app