For Confocal Microscope
images recorded with pinholes
of around 1 Airy disk
lateral sampling distances may be up to 1.6 times of the recommended Nyquist values without significantly compromising image quality.
When small pinholes
are used (< 0.5 Airy disk),
up to 1.3 times larger; with very large pinholes (> 4 Airy disks), up to 2 times.
These are xy and xz
of the 3D
point spread function
The 3D image dimensions (x × y × z) are
It was calculated using the Nyquist sample sizes specified above.
The number of samples (x × y × z) to cover this volume at the Nyquist rate is
To enhance dimmer regions the gamma
is corrected by 1.7 in the false color image.