Ernst Abbe's formula for the diffraction limit, set in stone at a monument in Jena. Source: Wikpedia Creative Commons.
Stefan W. Hell, Eric Betzig, and William E. Moerner have been awarded the
Nobel prize of Chemistry (2014) for developing microscopy techniques that beat the traditional
optical resolution limit.
Stimulated Emission Depletion (STED) and singe-molecule microscopy has a huge impact on fluorescence optical microscopy related research, allowing scientist to see more detail of their fluorescent labeled objects (see movie below).
SVI with Hans van der Voort, founder of SVI and co-author on several publications with Stefan Hell, congratulate him, and Eric Betzig and William E. Moerner with this big achievement!
A small selection of amazing Huygens deconvolution results on biological STED data is shown in this
STED Gallery