With colocalization analysis for fluorescence microscopy you can obtain quantitative information about the amount of spatial overlap between structures in different channels for 3D images and 3D-time series. As the spatial overlap can be defined in many ways, the Huygens Colocalization Analyzer gives you eleven different colocalization coefficients to determine spatial overlap. Learn more.

From Huygens Colocalization Analyzer 18.10 and newer, there are three options for the Colocalization Analyzer: The Wizard, Advanced, and Compact mode. All three modes have the same funcionalities only the presentation is different. The Wizard guides the users step by step, while the Advanced and Compact mode both provide an overview of all functions and data in a single window.

You can do that very easily:

• Via Colocalization Analyzer: Measure the Co-localization coefficients -> Go to Object Statistics -> select Analyze all
• Via Object Analyzer: Select Experimental preset -> Colocalization