Nearly all multi-channel images show at least some degree of chromatic aberration causing shifts in xyz, and possible scaling and rotation differences between fluorescent channels. These aberrations clearly affect any type of multi-channel analysis like colocalization and object-based analysis, and should thus be fixed. Huygens Chromatic Aberration Corrector quickly estimates, visualizes, and corrects chromatic aberrations. If you suspect any additional crosstalk/bleed-through in your multi-channel data, you may want to have a look at the Crosstalk Corrector as well.
Automatic and Easy
Estimation and correction with a few button clicks.
Shifts are visualized for easy verification.
Correction can be optimized for each specific image.
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Recording Beads is a good method to calibrate the existing chromatic aberration. The result of the analysis and correction delivered by the Chromatic Aberration Corrector on a beads image can easily be saved to a template and applied to any image taken under similar conditions. Left: MIP rendered projection of a Huygens deconvolved 3D widefield stack of 100nm 4-channel multicoloured beads (TetraSpeck - Life Technologies). Chromatic Aberration between channels can be more clearly measured and corrected after deconvolution. Right: MIP projection of the same image after using the Huygens Chromatic Aberration Corrector. Insets show a larger view of a single XZ slice of one bead.
HeLa cells stained with two different fluorophores. This image shows a shifts between the two different channels which has been corrected with the Chromatic Abberation Corrector . Raw image data was provided by Dr. Yury Belyaev, ALMF, EMBL Heidelberg, Germany
Huygens Chromatic Aberration Corrector shows a table with the values defining the shift, scaling and degrees of rotation
The image shows the nuclear pore complex. Left image show the deconvolved and chromatic aberration corrected image compared to the deconvolved image in the middle and raw image on the right. Image courtesy of Leica Microsystem
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