HUYGENS 2D & 3D Localizer (SMLM)
STORM/PALM/GSD/DNA PAINT analysis made Easy and Fast!
"Huygens Localizer is impressive. The PC/GPU we built mowed through 30,000 frame in under 60s. Pretty bad-ass!"
*Video displays a real-time and GPU accelerated run with the HUYGENS Localizer wizard. The easy-to-use and interactive user interface offers a very convenient workflow, and with high CPU and GPU parallelization, all your SMLM data will be processed at high speed.
Image: Microtubules imaged with a Leica SR GSD. Courtesy: Marko Lampe (image acquisition) / Ulf Matti (sample preparation), EMBL Heidelberg, Germany.
Easy & Interactive
Designed for maximum ease-of-use and supports various 2D and 3D localization data (STORM/PALM/GSD/DNA PAINT). Interactively optimize detection and processing settings, and obtain high-quality localization results in real-time.
All processing is highly parallelized for both CPU and GPU, allowing tens-of-thousands of time-frames to be processed per minute and save precious time in your image post-processing workflow.
2D + 3D SMLM package
Supports PALM, STORM, GSD & (DNA) PAINT data in various file-formats. Includes interactive pre- and post processing, various fitting algorithms, powerful background estimation methods, drift correction & interactive rendering and analysis. Batch SMLM processing and analysis of 3D (astigmatic) images is also supported
HUYGENS Localizer: Interactive & Fast Localization Microscopy processing
The HUYGENS Localizer is a stand-alone software package for fast & high performance processing of Single Molecule Localization Microscopy data (SMLM), such as Photoactivated Localization Microscopy (PALM), Stochastic Optical Reconstruction Microscopy (STORM), Ground State Depletion (GSD), and DNA PAINT images. The Huygens Localizer is optimally designed for CPU and GPU computation, enabling a real-time processing experience. Main functionalities include:
Interactive pre- and post-processing
Various fitting methods
Optional drift correction
Works both on CPU and GPU: highly parallelized performance
Visualization: interactive real-time rendering of localizations
Exporting localization data & rendered image
Interactive Object Analysis
Visualize the intensity flux over time, and crop useful region(s) from the total time-series. The HUYGENS Localizer is able to accurately estimate useful values from your image data, such as the photon-per-unit (ppu) value and the background. It can also estimate the PSF size from the microscopic parameters, which helps in more accurate localization results. In the particle detection stage, it is possible to interactively change settings such as threshold value to find optimal particle detection settings for your data.
Visualizing intensity flux (bleaching curve)
Interactive cropping in time
Powerful background estimation
Multiple CPU & GPU accelerated fitting methods
For optimal fitting, it is possible to choose from various fitting methods. All fitting methods are fully multi-threaded, and can run both on CPU-only as well as on an Nvidia GPU. The processing can be split up in block-sizes that are optimized for the (available) GPU RAM. This allows for efficient processing of tens of thousands of frames per minute.
Weighted Least-squares Fit
Center of mass
Learn: GPU acceleration
Interactive Post-processing, Rendering & Analysis
Interactively render the localized data into a super-resolution image. Change the pixel (binning) size, and choose between various (weighted) rendering settings: bin in nearest pixel, fixed Guass, weighted Gauss, width based on uncertainty. The automatic and accurate drift correction (no fiducial markers needed!) helps to automatically remove any thermal drift for the most accurate results. With multi-channel SMLM, a shift between the channels is typically present. The Huygens Chromatic Aberration Corrector can correct for this Aberration, resulting in more reliable multi-channel image analysis. With the interactive Object Analyzer (optional), it is possible to do object based statistic analysis. Measure for example: object count, distances, volume, surface, or overlap between objects in various channels.
Download now: get started!
You are always welcome to try out the HUYGENS Localizer on your microscope images. When you install the Huygens Suite on your computer, also feel free to try out our other products. You can also request pricing information or contact us for more information about the options best suited for your hardware and image processing needs.Download Now!