Huygens Object Tracker

Study 3D motion of cells and smaller particles in time series

Huygens Object Tracker tracks the movement of cells and particles in time series images. By manually selecting just a few objects and background regions, the machine learning algorithm in the Object Tracker is sufficiently trained to automatically detect new objects and to track these. Tracks can be filtered based on specific parameters, edited, and analyzed with the included Track Analyzer. Various properties of the measured tracks are presented in informative graphs and can be exported.

Image description:
FUCCI cells tracked for 50 seconds with Huygens Object Tracker. Courtesy of Dr. Richard Wubbolts, Center for Cell Imaging, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.


Easy to use

By manually selecting just a few objects and background regions, the machine learning algorithm is trained to identify all objects of similar type.

Detection options

Objects classification is based on various features, including brightness and size, and can be refined using additional filters.

Track Analyzer

Filter, edit, and analyze the tracks. The Track Analyzer presents the properties of all measured tracks in informative graphs and allows export of track data to a file.

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Main Features Object Tracker

Train machine learning algorithm with a few objects and background region.
Detect objects using brightness and other features.
Define thresholds on the number of objects, brightness, and width.
Use object geometry and orientation for connecting tracks.
Quick edit tracks (remove, break, connect).
Export tracks to Track Analyzer.
Save track detection settings to a template file to re-use.
Save tracks for future analysis in the Track Analyzer.

Main Features Track Analyzer

Analyze position; distance from track origin as histogram and time-plot, Mean Squared Displacement (MSD) and Diffusion Coefficient
Analyze speed; histogram, average speed, and x vs y velocity scatter plot.
Analyze flow; transport through a surface, defined using a line/poly-line in a Z-projection. Plot flux vs. time.
Analyze intensity of objects over time.
Analyze axial rotation and 3D angular speed of objects.
Export data to CSV file for any further analysis in e.g. Excel and Matlab.
Export graphs to EPS (Encapsulated PostScript) file.

Available for Huygens Essential & Professional

The Huygens Object Tracker can be tested with a free test license. The product is commercially available as a licensed option for Huygens Essential & Professional.

Why wait? Try out this option by downloading Huygens and request a test license, or receive pricing information.

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