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Scientific Volume Imaging B.V.

Scientific Volume Imaging

Deconvolution - Visualization - Analysis


™Huygens Image Quality Control

Automatically identifies issues in your image

Ensure your images meet high standards with this Image Quality Control (QC) tool! Whether you're unsure about your acquisition, curious about image quality, or monitoring your microscope's performance, this tool is your go-to solution. It automatically detects common issues in microscopic images, enhancing your scientific results.

Available in Huygens Essential and Professional, the QC tool thoroughly analyzes sample and bead (calibration) data, presenting clear explanations of potential problems. For bead images, it provides detailed point spread function (PSF) properties. Fix detected issues directly through the tool. A free online version with limited features is also available on this webpage.

QC Animation Gif V2

Automatic Quality Control

With just one click, scan your image for acquisition issues.

Immediate Correction

Following the QC analysis, promptly address any identified issues with Huygens restoration options.

Monitor microscope PSF

Simply load any bead image and obtain 5 different properties of your microscope PSF.

Quality Control of any image

Simply select a sample or bead/calibration image and start the QC tool. Within minutes a status report is presented with clear information about each identified issue, along with guidance on how to prevent and solve them.

Huygens QC option checks for the following image properties and issues:

Microscopic parameters
Relative Nyquist Rates
Signal-to-Noise (SNR) estimation
Clipping
Bleaching
Crosstalk
Hot and Cold pixels
Chromatic Aberration
Detector Alignment (useful for Array Detector data)
Stabilization (sample or stage drift)
PSF Shape (when using bead images, see below)

QC Huygens Screenshot
The main window of the Huygens Quality Control tool shows information about the image (top left and right), a general description on each property/issue that is checked (bottom left), and detailed information of the QC run (bottom right).
QCcheck Wide
Huygens Quality Control window showing a Leica SP82 confocal image of TetraSpeck1 beads ready to be subjected to a quality check for all the properties/issues as listed above. 1 TetraSpeck™ microspheres by Thermo Fisher Scientific. 2 Leica Microsystems

PSF shape analysis

the Huygens Quality Control option checks bead image data for all the issues mentioned. In addition, it assesses the Point-Spread-Function (PSF) quality of your microscope and generates a detailed report exportable as a CSV file for monitoring microscope performance. The report includes:

FWHM bead average
FWHM PSF
FWHM theoretical PSF
PSF asymmetry
PSF surface sphericity
PSF tilting
PSF skewed intensities

Note: PSF analysis in the QC option requires the PSF Distiller option.

The Huygens Image Quality Control tool is also available online for FREE!

Simply upload your image and recieve a QC report in PDF format.

Huygens complete Toolbox for Image Restoration

The Quality Control tool seamlessly incorporates the various Huygens restoration options, providing the most effective and straightforward method to not only identify, but also to correct the potential issues in your data. Some options are already present in Essential and Professional, whereas others can be added as a option.

Huygens Hot&Cold Pixel Remover
Huygens Bleaching Corrector
Huygens Crosstalk Corrector (option)
Huygens Chromatic Aberration Corrector (option)
Huygens Object Stabilizer (option)
Huygens PSF Distiller (option)