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Scientific Volume Imaging B.V.

Scientific Volume Imaging

Deconvolution - Visualization - Analysis


™Huygens Image Quality Control

Automatically identifies issues in your image

If you're uncertain about your acquisition, unsure how check the quality of your image, or aiming to monitor your microscope's performance, this unique Huygens Image Quality Control option is your invaluable companion! Automatically detecting common pitfalls affecting image quality in every microscopic image, this tool improves your science.

The Image Quality Control (QC) option is available for both Huygens Essential and Professional. It meticulously searches for a wide array of imaging issues in both sample and bead (calibration) data. Comprehensive details of potential issues are clearly presented and explained. In case of bead images, a broad set of point spread function (PSF) properties are provided. Options to promptly fix the detected issues can be directly accessed from the QC tool. A free online version of the QC tool with limited functionality is available on this webpage.

QC Animation Gif V2

Automatic Quality Control

With just one click, scan your image for a broad spectrum of acquisition issues.

Immediate Correction

Following the Quality Control analysis, you can promptly address any identified issues using the corresponding Huygens options.

Monitor microscope PSF

The Quality Control option can also be used to analyse various properties of your microscope PSF.

Quality Control of any image

Simply select a sample or bead/calibration image and start the QC tool. Within minutes a status report is presented with clear information about each identified issue, along with guidance on how to prevent and solve them.

Huygens QC option checks for the following image properties and issues:

Microscopic parameters
Relative Nyquist Rates
Signal-to-Noise (SNR) estimation
Clipping
Bleaching
Crosstalk
Hot and Cold pixels
Chromatic Aberration
Detector Alignment (useful for Array Detector data)
Stabilization
PSF Shape (when using bead images, see below)

QC Huygens Screenshot
The main window of the Huygens Quality Control tool shows information about the image (top left and right), a general description on each property/issue that is checked (bottom left), and detailed information of the QC run (bottom right).
QCcheck Wide
Huygens Quality Control window showing a Leica SP82 confocal image of TetraSpeck1 beads ready to be subjected to a quality check for all the properties/issues as listed above. 1 TetraSpeck™ microspheres by Thermo Fisher Scientific. 2 Leica Microsystems

PSF shape analysis

Huygens Quality Control option is able to check bead image data for all the issues described above. In addition, it determines the level of the Point-spread-function (PSF) quality of your microscope, and offers a full report which can be exported as a CSV file for monitoring the microscope performance. This report includes the following parameters:

FWHM bead average
FWHM PSF
FWHM theoretical PSF
PSF asymmetry
PSF surface sphericity
PSF tilting
PSF skewed intensities

Note: PSF shape analysis in the QC option requires the PSF Distiller option.

The Huygens Image Quality Control tool is also available online for FREE!

Simply upload your image and recieve a QC report in PDF format.

Huygens tools for Image Restoration

The Quality Control tool seamlessly incorporates the various Huygens restoration options, providing the most effective and straightforward method to not only identify, but also to correct the potential issues in your data. Some options are already present in Essential and Professional, whereas others can be added as a option.

Huygens Hot&Cold Pixel Remover
Huygens Bleaching Corrector
Huygens Crosstalk Corrector (option)
Huygens Chromatic Aberration Corrector (option)
Huygens Object Stabilizer (option)
Huygens PSF Distiller (option)