Huygens Object Analyzer
Obtain reliable object statistics from 3D-5D images.
Objects in 3D-5D (multichannel, time series) images can be quickly and easily analyzed with Huygens Object Analyzer. Statistics of objects are produced with just a few button clicks. Object segmentation - which is often considered being a challenging and subjective task - is made easy with algorithms that set the seed and threshold levels optimally, offering instant feedback like number of objects per channel. Additional watershed tools, object filtering, and advanced region of interest (ROI) settings are at hand to fine-tune 3D object segmentation. The segmented objects are directly visualized by the high-end continuous Iso Surface Ray Tracing renderer and ready for immediate analysis. Various analysis modes and presets are available to quickly answer your specific research question.
Image description
3D volume analysis of kinetochore complex structures involved in cell division. Image kindly provided by Livio Kleij (facility) and Martijn Vroomans, Medical Oncology, UMC Utrecht, The Netherlands.
3D volume analysis of kinetochore complex structures involved in cell division. Image kindly provided by Livio Kleij (facility) and Martijn Vroomans, Medical Oncology, UMC Utrecht, The Netherlands.
Interactive Scene
Rotate, analyze, discard and anchor objects using only your mouse cursor.
Easy Segmentation
Obtain reliable results with the advanced segmentation, ROI, and watershed options.
Many Object Statistics
Object statistics in a clear table and graph, and can be exported for further analysis.
Testimonial
Both my facility users and I are very impressed with the new (and not so new) features, and some of my new users are really enjoying the object analyzer and the ROI options!
Dr. Zoltan Cseresnyes, research associate and co-manager Confocal and 2-Photon Microscopy Core Facility, MDC Berlin, Germany.
Dr. Zoltan Cseresnyes, research associate and co-manager Confocal and 2-Photon Microscopy Core Facility, MDC Berlin, Germany.
Main features
- Automatic segmentation based on the image histogram
- Watershed segmentation algorithm
- On-the-fly calculation of number of objects
- Measure volume, surface, and geometry/dimensions of objects
- Colocalization at the object level
- Measure distances between nearest object and a pre-defined reference object
- Interactive definition of Regions Of Interest (ROI)
- Measure object properties inside the ROI
- Configurable parameter list, that can be stored in user presets
- Advanced object filter based on any of the reported parameters
- Exportable table with statistics
Use in research
Aoyama-Ishiwatari, S, Okazaki, T, Iemura, S et al. NUDT21 links mitochondrial IPS-1 to RLR-containing stress granules and 3 activates host antiviral defense.Huygens was used for deconvolution and 3D object volume counting with the Object Analyzer
J. Immunol. 206, 154-163 (2021)
Berndt-Paetz, M, Herbst, L, Weimann, A et al. IC/BPS-associated alterations of M2 and M3 muscarinic acetylcholine receptor trafficking in human detrusor.
Analysis of 3D Object volume and receptor membrane localization was performed with Huygens deconvolution and the Object Analyzer.
Neurourol. Urodyn. 38, 1818- 1827 (2019)
For more, see Scientific Publications
Related product
Images may contain misalignments between 3D slices or between time frames, which hinder object analysis. Such misalignments can be corrected with the Huygens Object Stabilizer.Object Stabilizer
More information
Expert tutorialSupport page
Webinar Object Analyzer