Huygens Object Analyzer
Obtain reliable object statistics from 3D-5D images.
Objects in your 3D-5D (multichannel, time series) images can be quickly and easily analyzed with Huygens Object Analyzer. Statistics of objects are produced within seconds with just a few button clicks. Object segmentation - which is often considered being a challenging and subjective task - is made easy with algorithms that set the seed and threshold levels optimally, offering instant results like number of objects per channel. Additional watershed tools, object filtering, and advanced region of interest (ROI) settings are at hand to fine-tune 3D object segmentation. The segmented objects are directly visualized by the high-end continuous Iso Surface Ray Tracing renderer and can be immediately analyzed. Many analysis modes and presets are available to help you quickly answering your specific research question.
Interactive Scene
Objects can be viewed from all angles, analyzed, discarded, and set as a reference anchor, using only your mouse cursor
Easy Segmentation
Advanced segmentation, ROI, and watershed options allow you to obtain the most reliable results from your valuable data
Many Object Statistics
All kinds of object statistics are presented within seconds in a clear table and graph, and can be exported as a text, csv, or MatLab file for further analysis.
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References
Huygens was used for deconvolution and 3D object volume counting of stress granules (SG).Aoyama-Ishiwatari S, Okazaki T, Iemura S-i, Natsume T, Okada Y, Gotoh Y. NUDT21 links mitochondrial IPS-1 to RLR-containing stress granules and 3 activates host antiviral defense.
Link: bioRxiv, preprint April 2020
Huygens was used for deconvolution, and Golgi organization was analyzed with Huygens Object Analyzer
Vibha Singh, Chaitanya Erady, Nagaraj Balasubramanian, Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells.
Link: J Cell Sci July 27, 2018.
Analysis of 3D Object volume and receptor membrane localization was performed with Huygens deconvolution and the Object Analyzer.
Berndt-Paetz M, Herbst L, Weimann A, Gonsior A, Stolzenburg JU, Neuhaus J. IC/BPS‐associated alterations of M2 and M3 muscarinic acetylcholine receptor trafficking in human detrusor.
Link: Neurourology & Urodynamics 2019; 1-10.
Huygens deconvolution and Object Analyzer was used to determine the spatial relationships between fluorescent AII amacrine cell and immunolabeled punctae.
Hartveit E., Zandt B-J., Madsen E., Castilho A., Mørkve S.H., Veruki M.L. AMPA receptors at ribbon synapses in the mammalian retina: kinetic models and molecular identity.
Link: Brain Struct Funct. 2018 Mar; 223(2):769-804.
For more references see: Scientific References
Available for Huygens Essential & Professional
The Huygens Object Analyzer option can be tested with a free test license. The product is commercially available as a licensed option for Huygens Essential, Professional.Why wait? Try out this option by downloading Huygens and request a test license, or receive pricing information.
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