Huygens Object Analyzer
Obtain reliable object statistics from 3D-5D images.
Objects in 3D-5D (multichannel, time series) images can be quickly and easily analyzed with Huygens Object Analyzer. Statistics of objects are produced with just a few button clicks. Object segmentation - which is often considered being a challenging and subjective task - is made easy with algorithms that set the seed and threshold levels optimally, offering instant feedback like number of objects per channel. Additional watershed tools, object filtering, and advanced region of interest (ROI) settings are at hand to fine-tune 3D object segmentation. The segmented objects are directly visualized by the high-end continuous Iso Surface Ray Tracing renderer and ready for immediate analysis. Various analysis modes and presets are available to quickly answer your specific research question.
Image description:
3D volume analysis of kinetochore complex structures involved in cell division. Image kindly provided by Livio Kleij (facility) and Martijn Vroomans. Medical Oncology, UMC Utrecht, The Netherlands.
Interactive Scene
Objects can be viewed from all angles, analyzed, discarded, and set as a reference anchor, with only your mouse cursor.
Easy Segmentation
Advanced segmentation, ROI, and watershed options allow you to obtain the most reliable results from your valuable data.
Many Object Statistics
Object statistics are presented within seconds in a clear table and graph, and can be exported as a text, csv, or MatLab file for further analysis.
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Main features
- Automatic segmentation based on the image histogram
- Watershed segmentation algorithm
- On-the-fly calculation of number of objects
- Measure volume, surface, and geometry/dimensions of objects
- Colocalization at the object level
- Measure distances between nearest object and a pre-defined reference object
- Interactive definition of Regions Of Interest (ROI)
- Measure object properties inside the ROI
- Configurable parameter list, that can be stored in user presets
- Advanced object filter based on any of the reported parameters
- Exportable table with statistics
Scientific References
Huygens was used for deconvolution and 3D object volume counting of stress granules (SG).Aoyama-Ishiwatari et al. NUDT21 links mitochondrial IPS-1 to RLR-containing stress granules and 3 activates host antiviral defense. BioRxiv, preprint April 2020
Huygens was used for deconvolution, and Golgi organization was analyzed with Huygens Object Analyzer
Vibha Singh et al. Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells. J Cell Sci July 27, 2018.
Analysis of 3D Object volume and receptor membrane localization was performed with Huygens deconvolution and the Object Analyzer.
Berndt-Paetz et al. IC/BPS‐associated alterations of M2 and M3 muscarinic acetylcholine receptor trafficking in human detrusor. Neurourology & Urodynamics 2019; 1-10.
Huygens deconvolution and Object Analyzer was used to determine the spatial relationships between fluorescent AII amacrine cell and immunolabeled punctae.
Hartveit et al. AMPA receptors at ribbon synapses in the mammalian retina: kinetic models and molecular identity. Brain Struct Funct. 2018 Mar; 223(2):769-804.
For more see: Scientific References
Available for Huygens Essential & Professional
The Huygens Object Analyzer option can be tested with a free test license. The product is commercially available as a licensed option for Huygens Essential, Professional.Why wait? Try out this option by downloading Huygens and requesting a test license, or receiving pricing information.
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