Huygens Re-scan Confocal Optical Option

Specially suited for deconvolving Re-Scan and VisiTech instant-SIM images

Huygens offers a specialized Huygens Deconvolution option to optimally process Re-scan Confocal Microscopy (RCM) images. The Huygens Re-scan Optical Option can be added to a Huygens license as a separate option and improves acquired RCM images signficiantly. Good deconvolution results have also been reported when using the RCM deconvolution option for VisiTech instant-SIM (VT-iSIM) images.

Images show individual chromosomes of a nuclear spread from fixed mouse spermatocytes, immunostained for SYCP3 a component of the synaptonemal complex (Alexa 488-labelling). Raw images were acquired with RCM without re-scanning (left) and with re-scanning (middle). The raw RCM image was deconvolved with the Huygens Re-scan Optical Option (right). Sample courtesy of A. Agostinho and C. Höög, Karolinska Institutet, Sweden; imaging and Huygens Deconvolution performed by Erik Manders, Confocal.nl, Netherlands.

About the Re-scan Confocal Microscopy system

The RCM system is an affordable add-on system developed and sold by Confocal.nl. This system can turn any fluorescent microscope into a 3D confocal system. The commercial add-on system is based on the Re-scan paper published by De Luca et al. For more information on the RCM system, see the Confocal.nl website.

Use in research

Lucidi M, Hristu R, Nichele L, Stanciu G.A et al., Characterization of Acinetobacter baumannii Filamentous Cells by Re-Scan Confocal Microscopy and Complementary Fluorometric Approaches.
Huygens was used to deconvolve Confocal and Re-Scan Confocal (RCM) images.
IEEE J. Sel. Top. Quantum Electron 27, 1-7 (2021)

For more, see Scientific Publications

The best deconvolution results can be achieved by using a measured PSF extracted with the Huygens PSF Distiller. After deconvolution, images can be used for reliable image analysis, for example with the Huygens Object Analyzer.

PSF Distiller Object Analyzer

More information

Introduction to deconvolution
Huygens Deconvolution
Deconvolution images