Huygens Re-scan Confocal Optical Option
Since Huygens version 18.10, SVI also offers a specialized Huygens deconvolution option to optimally process Re-scan Confocal Microscopy (RCM) images. The Huygens Re-scan Optical Option can be added to a Huygens license as a separate option, and improves acquired RCM images signficiantly.
Images show individual chromosomes of a nuclear spread from fixed mouse spermatocytes, immunostained for SYCP3 a component of the synaptonemal complex (Alexa 488-labelling). Raw images were acquired with RCM without re-scanning (confocal) and with re-scanning (RCM). The raw RCM image was deconvolved with the Huygens Re-scan Optical Option.
Sample courtesy of A. Agostinho – Advanced Light Microscopy Facility, Science for Life Laboratory and C. Höög – Department of Cell and Molecular Biology, Karolinska Institutet (Sweden). Imaging and Huygens deconvolution was performed by Erik Manders (Confocal.nl).
About the Re-scan Confocal Microscopy system
The RCM system is an affordable add-on system developed and sold by Confocal.nl. This system can turn any fluorescent microscope into a 3D confocal system. The commercial add-on system is based on the Re-scan paper published by De Luca et. al (1). For more information on the RCM system, see the Confocal.nl
(1) G. M. R. De Luca, R. M. P. Breedijk, et al. “Re-scan Confocal Microscopy: scanning twice for better resolution,” Biom. Optics Express 4(11), 2644-2656 (2013).