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Huygens Light Sheet Deconvolution Software

Supports many types of light sheets


Raw Light Sheet
Huygens Deconvolved

Huygens Light Sheet Fluorescence Microscopy (LSFM) deconvolution option supports a range of light sheets types, such as a Gaussian-profile (incl. multiview and flat field), scanning beam, and scanning Bessel beam and lattice. Large LSFM data sets benefit greatly from Huygens efficient RAM use, robust deconvolution algorithms, and GPU acceleration. Huygens Light Sheet deconvolution involves a spatially variant PSF for correcting PSF differences due to changes in light sheet thickness and depth-dependent spherical aberration. Deconvolution can be combined with multi-view fusion, made extremely easy with Huygens Fuser.

Image description:
Image of Drosophila brain taken with Zeiss Z1 Light Sheet microscope. This multiview dataset consisting of eight views (one of the raw views is shown) was deconvolved and fused with the Huygens Software. Image kindly provided by Dr. Denis Ressnikoff, University Claude Bernard, Lyon, France.

All Microscope Brands

All brands of light sheet microscopes are supported, as well as many File Formats.

Improve Resolution

Improve the quality and resolution. With a PSF adapted to local light sheet thickness.

MLE Algorithms

Use advanced Maximum Likelihood Estimation algorithms. Learn more: Huygens Deconvolution Algorithms.


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Testimonials & References

"Ultra-thin fluorocarbon foils optimise multiscale imaging of three-dimensional native and optically cleared specimens."
Hötte K, Koch M, Hof L, Tuppi M, Moreth T, Verstegen MMA, van der Laan LJW, Stelzer EHK, Pampaloni F. (2019).
Link: Sci Rep 9, 17292 (2019)
Huygens was used for Light Sheet fusion and deconvolution

"Deconvolution of Very Large Data Sets"
Ponti A, Sevilla Sanchez D, Belyaev Y. (2015).
Link: Imaging & Microscopy (2015) 12:22-24
This article shows that deconvolution of large datasets like SPIM data, can be easily performed with the Huygens Remote Manager/Core.

SPIM GIF
Maximum Intensity Projection of a raw (left) and deconvolved (right) 3D image from mouse blastocysts acquired with a Digital Light Sheet microscope. Deconvolution was performed with the CMLE algorithm and the Huygens module for calculating the theoretical Light Sheet point-spread-function. Image was kindly provided by Dr. Marc Duque Ramirez and Dr. Ritsuya Niwayama (Hiiragi group) and Dr. Stefan Terjung (ALMF) from the EMBL Heidelberg, Germany.

See more: Images in the field of Genetics & Developmental Biology
Huygens Fused + Deconvolved
Raw SPIM
The image shows tubulin in the Arabidopsis plant leaf. You can see the somata clearly. Two separate images were acquired with illumination from opposing sides using the Zeiss Z1 Light Sheet. The raw czi images were deconvolved and fused with Huygens. Image kindly provided by Dr. Shingo Nagawa, Cell Biology Core Facility, Shanghai Center for Plant Stress Biology, CAS, China.

See more: Images in the field of Plant Biology

Fuser Exaples
Two examples of Zeiss Z1 Light Sheet datasets deconvolved and fused with the Huygens FUSER. The first dataset is from a Drosophila brain acquired at 360 degrees rotation (45 degrees steps), and the second set is from a chicken embryo imaged from two opposing sides. Image was kindly provided by Prof. Christophe Marcelle, Mrs. Marie Julie Dejardin (INMG) & Dr. Denis Ressnikoff (CIQLE), Université Lyon 1, France.

See more: Images in the field of Genetics & Developmental Biology
Results Fusion+cmle(left)
The image shows tubulin in the Arabidopsis plant leaf. You can see the somata clearly. Two separate images were acquired with illumination from opposing sides using the Zeiss Z1 Light Sheet. The raw czi images were deconvolved and fused with Huygens. Image kindly provided by Dr. Shingo Nagawa, Cell Biology Core Facility, Shanghai Center for Plant Stress Biology, CAS, China.

See more: Images in the field of Plant Biology

Comparison
Huygens deconvolution of high resolution LIght Sheet data. GFP labeled yolk granules in a C. elegans one-cell stage embryo before (left) and after deconvolution with the CMLE algorithm using a theoretical Light Sheet point-spread-function. Image kindly provided by Dr. Uros Krzic, Dr. Lars Hufnagel, and Dr. Yury Belyaev, European Molecular Biology Laboratory, Heidelberg, Germany.

See more: Images in the field of Genetics & Developmental Biology


Available for Huygens Essential, Professional and Core

The Huygens Light Sheet optical option supports all brands of Light Sheet or Selective Plane Illumination Microscopy (SPIM) microscopes. The option can be tested with a free test license. The product is commercially available as a licensed option for Huygens Essential, Professional and Core.

Why wait? Try out this option by downloading Huygens and request a test license, or receive pricing information.

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