Huygens Confocal Deconvolution Software

Specially designed for single point-scanning Confocal Microscopes

Raw Confocal
Huygens Deconvolved

Single point-scanning Confocal Microscope images can be sucessfully deconvolved within the Huygens Software, offering a significant improvement in object resolution in x, y and z, an increase in signal, and a correction of noise. As a result, images will be easier to segment for optimal visualization and analysis. The gain in signal that you can obtain with deconvolution also allows you to image faster and have less photoxicity and bleaching. Huygens offers state-of-the art implementations of the Classic Maximum Likelihood Estimation (CMLE), and the Good's roughness Maximum Likelihood Estimation (GMLE) algorithms, which work very fast and efficiently on noisy image data.

Image description:
Confocal image showing basal bodies of the cilia in a paramecium. Image was provided by A. Aubusson-Fleury, CNRS, Gif sur Yvette, Paris.

All Microscope Brands

All brands of single point-scanning Confocal Microscopes are supported, as well as many File Formats.

Reach 120nm Resolution

120nm resolution is achievable with Huygens.

MLE Algorithms

Use advanced Maximum Likelihood Estimation algorithms. Learn more: Huygens Deconvolution Algorithms


Our confocal microscopy users are really impressed by the program! We find it very important for our research with yeast cells and bacteria

Dr. Christina Schlatterer, SFB969/LS Deuerling, University of Konstanz, Germany.
Researchers who use Huygens at the facility to enhance their confocal data are very much impressed by the results.

Dr. Ulrike Engel, Scientific Director of the NIC Imaging Centre, Bioquant, University of Heidelberg, Germany.

Image 1
Image of DAPI (Red) and mab22C10 (Green) staining in larval brain of Drosophila melanogaster. The image was captured using Leica SP5II confocal microscope. Image was kindly provided by Dr. Anand Krishna Tiwari, Indian Institute of Advanced Studies, India.

See more: Images in the field of Neurosciences
Screenshot From 2020 02 21 16 51 19
Yeast organelle transport. Time series acquisition, Single point-scanning Confocal. Late-Golgi marker Sec7-DsRed, Early Golgi marker Cog1-GFP. Perfectly acquired, but noisy confocal time series of low abundance proteins in yeast endosomal and Golgi system allows accurate object analysis after deconvolution with Huygens. Image kindly provided by Prof. Benjamin S. Glick, MGCB, University of Chicago, United States.

See more: Images in the field of Cell Biology

Image 2 PMEF Tm5NM1+2 KO Huygens Deconvolved. Contrast Enhanced Max Projection
Image shows the remarkable complexity of the actin filament network in primary mouse embryonic fibroblasts (pMEF) null for the actin regulatory proteins Tm5NM1/2. Actin filaments are visualised by phalloidin. Confocal image (N.A1.4. 100x) was deconvolved and visualised with Huygens Professional. Image kindly provided by Mr. Howard Vindin. Cellular & Genetic Medicine Unit, School of Medical Sciences, University of New South Wales, Australia.

See more: Images in the field of Developmental Biology
A series of developing Drosophila oocytes (egg-chambers to be precise). A Z-stack of 116 optical sections was taken on a Leica SP8 Laser scanning confocal microscope with a 63x 1.4NA objective. Actin (in yellow) is stained with rhodamin phalloidin and the nuclear envelopes of the so -called nurse cells are labeled with autofluorescence of GFP-Tm1 (green). HRM was used to deconcolve the raw image. Image kindly provided by Dr. Imre Gaspar, Developmental Biology Unit, EMBL Heidelberg, Germany.

See more: Images in the field of Developmental Biology

Available for Huygens Essential, Professional and Core

The Huygens Confocal optical option supports all brands of single point-scanning confocal microscopes. The option can be tested with a free test license. The product is commercially available as a licensed option for Huygens Essential, Professional and Core.

Why wait? Try out this option by downloading Huygens and request a test license, or receive pricing information.

Pricing Info Request Trial