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Scientific Volume Imaging B.V.

Scientific Volume Imaging

Deconvolution - Visualization - Analysis


™Huygens Spinning Disk Deconvolution Software

Includes new pinhole size and spacing calculator tool

Huygens Deconvolved
RAW Spinning Disk
Many researchers use Huygens to routinely correct for noise, blur, and low signal within Spinning Disk microscopy images, including Yokogawa SoRa data. The Spinning Disk deconvolution option of Huygens is unique in that it includes a pinhole spacing parameter that considers the crossover of signal between neighboring pinholes on the disk which is detrimental to the quality of the raw image data. Huygens deconvolution significantly increases the quality, contrast, and resolution in xyz images. As a result, images will be easier to visualize and subsequent analysis is more reliable. Because image signal can increase manifold, Huygens even allows you to image faster at lower light dose, which minimizes bleaching and phototoxicity.
Image description Hela cell stained for actin and the nucleus was imaged with an Andor Spinning Disk and deconvolved and visualized with Huygens, Courtesy: Raffaella Vaccaroli and Andreas Girod, Light Microscopy Facility - Life Sciences Research Unit, University of Luxembourg.

Improve Resolution

Improving image quality and resolution. Learn more: Huygens Deconvolution.

Multiple Algorithms

Use advanced Maximum Likelihood Estimation algorithms. Learn more: Huygens Algorithms.

All Microscope Brands

All brands and types of Spinning Disk Microscopes are supported, as well as many File Formats.

Testimonials

It’s been great to see how Huygens has made possible several projects that would otherwise have been very challenging or impossible. Having access to Huygens and the quick and experienced support from SVI gives staff at QBI a strong advantage in their microscopy based research. Dr. Luke Hammond, facility manager of the Advanced Microscopy Facility, Queensland Brain Institute, Australia
Researchers who use Huygens at the facility to enhance their confocal data are very much impressed by the results. As noisy data is the rule rather than the exception with live samples, we now use Huygens routinely to enhance our spinning disk data. Dr. Ulrike Engel, scientific director of the Nikon Imaging Centre, University of Heidelberg, Germany.

SoRa Microscopes

SoRa microscopes are great for providing fast imaging of live samples with a high resolution. Huygens deconvolution further improves the quality of your data in each of these dimensions, using a PSF that is optimized for the SoRa principle (optical reassignment).

high resolution The inventors of SoRa, the Yokogawa Electric Corporation, already recognized the potential of using deconvolution, preferring Huygens Deconvolution on biological data over alternatives, and demonstrating a lateral resolution of 113 nanometer with Huygens in their paper1.

compensate chromatic aberrations The SoRa system uses a microlens array to achieve a higher resolution, but this comes at the cost of having higher chromatic aberrations as the microlenses are not achromatic1. Huygens can fully compensate for this inherent drawback using the chromatic aberration corrector.

even longer live experiments SoRa benefits from the signal-to-noise enhancement of deconvolution. This means that the acquisition time can be decreased without loss of information in the final image (See: Does deconvolution work on noisy data?). For live samples, this can be leveraged to image longer or faster without risk of photobleaching or phototoxicity due to prolonged light exposure.

Use in research

1 Takuya Azuma and Takayuki Kei, Super-resolution spinning-disk confocal microscopy using optical photon reassignment. Huygens was used by the inventors of SoRa Opt. Express 23, 15003-15011 (2015)

2 Moore A.S, Coscia S.M, Simpson C.L et al., Actin cables and comet tails organize mitochondrial networks in mitosis. Huygens was used for spinning disk deconvolution. Nature 591, 659–664 (2021)

3 Jiamin Wu, Zhi Lu, Dong Jiang et al., Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale. Huygens was used for 3D light-sheet and spinning-disk confocal deconvolution. Cell (2021)

For more, see Scientific Publications

The high scanning speed makes Spinning Disk Confocal Microscopy well fit for live cell imaging. Unwanted movements can be corrected for with the Huygens Object Stabilizer while movements of interest can be tracked with the Huygens Object Tracker.

Object Stabilizer Object Tracker

More information

Introduction to deconvolution Huygens Deconvolution Deconvolution images

Raw Spinning Disk
Huygens Deconvolved
Hela cell stained for actin and the nucleus was imaged with an Andor Spinning Disk. Within this image a two-point resolution improvement of at least 1.5 times was measured. Image kindly provided by Dr. Raffaella Vaccaroli and Dr. Andreas Girod, Light Microscopy Facility - Life Sciences Research Unit, University of Luxembourg.

See more: Images in the field of Cell Biology

Pawel Pasierbek Image contest 2015 winner
This cultured neuron was imaged with a spinning disk confocal and deconvolved with Huygens. Image kindly provided by Dr. Pawel Pasierbek, Institute of Molecular Pathology, BioOptics (IMP, IMBA, GMI), Vienna, Austria.

See more: Images in the field of Neurosciences

MoorePhotoentrySmall
This image shows two metaphase HeLa cells expressing Mito-DsRed2 and LifeAct-GFP, and labeled with Hoechst dye to visualize DNA. These live cells were imaged by spinning disk confocal microscopy and deconvolved in Huygens. Image kindly provided by Andy Moore, Holzbauer Lab, University of Pennsylvania, United States.

See more: Images in the field of Cell Biology

Tucker Rutledge NIEHS
Standard Invitrogen Floucells #1 prepared slide, containing bovine pulmonary artery endothelial cells stained for mitochondria (red), F-actin (green), and nuclei (blue). Before (left) and after (right) deconvolution images were merged side by side to display the power of deconvolution. Image kindly provided by Dr. Jeff Tucker and Dr. Holly Rutledge from NIEHS, NIH, USA. See more: Images in the field of Cell Biology