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Huygens Spinning Disk Deconvolution Software

Specially designed to take into account the optical properties of all brands of microscopes



Huygens Deconvolved
RAW Spinning Disk
Many researchers use Huygens Spinning Disk option routinely to correct for noise, blur, and low signal. Spinning Disk microscopy images, including Yokogawa SoRa data, can be deconvolved with the Huygens Software with truly stunning results. Huygens deconvolution significantly increases the quality, contrast, and resolution in xyz images. As a result, images will be easier to visualize and subsequent analysis is more reliable. Because image signal can increase manyfold, Huygens allows you to image faster at lower signal which minimizes bleaching and phototoxicity.

Image description
Hela cell stained for actin and the nucleus was imaged with an Andor Spinning Disk and deconvolved and visualized with Huygens, Courtesy: Raffaella Vaccaroli and Andreas Girod, Light Microscopy Facility - Life Sciences Research Unit, University of Luxembourg.


Improve Resolution

Improving image quality and resolution. Learn more: Huygens Deconvolution.


Multiple Algorithms

Use advanced Maximum Likelihood Estimation algorithms. Learn more: Huygens Algorithms.


All Microscope Brands

All brands and types of Spinning Disk Microscopes are supported, as well as many File Formats.




Testimonials

It’s been great to see how Huygens has made possible several projects that would otherwise have been very challenging or impossible. Having access to Huygens and the quick and experienced support from SVI gives staff at QBI a strong advantage in their microscopy based research.

Dr. Luke Hammond, facility manager of the Advanced Microscopy Facility, Queensland Brain Institute, Australia
Researchers who use Huygens at the facility to enhance their confocal data are very much impressed by the results. As noisy data is the rule rather than the exception with live samples, we now use Huygens routinely to enhance our spinning disk data.

Dr. Ulrike Engel, scientific director of the Nikon Imaging Centre, University of Heidelberg, Germany.



Use in research

Moore A.S, Coscia S.M, Simpson C.L et al., Actin cables and comet tails organize mitochondrial networks in mitosis.
Huygens was used for spinning disk deconvolution.
Nature 591, 659–664 (2021)

Jiamin Wu, Zhi Lu, Dong Jiang et al., Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale.
Huygens was used for 3D light-sheet and spinning-disk confocal deconvolution.
Cell (2021)

For more, see Scientific Publications

The high scanning speed makes Spinning Disk Confocal Microscopy well fit for live cell imaging. Unwanted movements can be corrected for with the Huygens Object Stabilizer while movements of interest can be tracked with the Huygens Object Tracker.

Object Stabilizer Object Tracker

More information

Introduction to deconvolution
Huygens Deconvolution
Deconvolution images



Raw Spinning Disk
Huygens Deconvolved
Hela cell stained for actin and the nucleus was imaged with an Andor Spinning Disk. Within this image a two-point resolution improvement of at least 1.5 times was measured. Image kindly provided by Dr. Raffaella Vaccaroli and Dr. Andreas Girod, Light Microscopy Facility - Life Sciences Research Unit, University of Luxembourg.

See more: Images in the field of Cell Biology

Pawel Pasierbek Image contest 2015 winner
This cultured neuron was imaged with a spinning disk confocal and deconvolved with Huygens. Image kindly provided by Dr. Pawel Pasierbek, Institute of Molecular Pathology, BioOptics (IMP, IMBA, GMI), Vienna, Austria.

See more: Images in the field of Neurosciences


MoorePhotoentrySmall
This image shows two metaphase HeLa cells expressing Mito-DsRed2 and LifeAct-GFP, and labeled with Hoechst dye to visualize DNA. These live cells were imaged by spinning disk confocal microscopy and deconvolved in Huygens. Image kindly provided by Andy Moore, Holzbauer Lab, University of Pennsylvania, United States.


See more: Images in the field of Cell Biology
Tucker Rutledge NIEHS
Standard Invitrogen Floucells #1 prepared slide, containing bovine pulmonary artery endothelial cells stained for mitochondria (red), F-actin (green), and nuclei (blue). Before (left) and after (right) deconvolution images were merged side by side to display the power of deconvolution. Image kindly provided by Dr. Jeff Tucker and Dr. Holly Rutledge from NIEHS, NIH, USA.

See more: Images in the field of Cell Biology