Tile Stitching & Deconvolution with Huygens. Large biological samples often require stitching to create high-resolution images of the entire sample. The Huygens Stitcher provides an intuitive and reliable solution for high-quality stitching across all microscopy data types. It also includes advanced features like deconvolution and vignetting correction for superior results. With its unique 3D tile positioning method, the Huygens Stitcher ensures precise stitching, even with 0% acquisition overlap between tiles. GPU acceleration speeds up the process, and the new "low-memory" option (introduced in version 24.10) optimizes processing in X, Y, and Z, significantly reducing memory demands for 3D optimization. Join us for this webinar to see a live demonstration of Huygens' stitching and deconvolution features and learn how they can transform your imaging workflow!
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The array detector is a major advancement in fluorescent microscopy, solving the signal loss issue from confocal microscope pinholes. Although it adds complexity to image processing due to multiple detectors, this is a minor trade-off. Deconvolution becomes more difficult because each detector captures an image with a different PSF. To address this, we've developed a new Huygens deconvolution module that accommodates these PSF variations for STED and confocal images. In this webinar, we will explain this deconvolution method, its advantages over standard approaches, and how it differs from our previous Airyscan method. We’ll also demonstrate Huygens' SuperXY mode, which uses information from 23 (or 32) shifted detector images to split voxels into four subpixels, reducing photodamage from the STED laser while preserving resolution, enabling new super-resolution experiments.
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The Huygens Workflow Processor is your go-to solution for reliable and reproducible quantitative image analysis in batch. Create a single image processing pipeline to address all your analysis needs, combining tasks like Colocalization, Object Analysis, and advanced image restoration or deconvolution. With the interactive Workflow Designer, you can easily build complete workflows using a simple drag-and-drop interface. Tasks are automatically arranged in their optimal order within the workflow, ensuring efficiency. Once your workflow is set, you can schedule it for individual images or entire folders, including nested directories. The process runs automatically, applying the workflow to all selected images. Progress is easy to track with real-time previews of both raw and restored images.
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Are you currently using other software and feeling uncertain about the results? Perhaps you’re already working with Huygens but think you could use it more efficiently, or maybe you’re planning to start using Huygens and want to learn more. Even if you’re just curious, this free virtual workshop is the perfect opportunity to explore what Huygens can do for you. In just three hours, you may see object details you’ve never seen before, significantly improve the quality of your live-cell imaging experiments, create visuals worthy of an Image Contest, and make object segmentation much easier. All of this is aimed at helping you to achieve more scientifically honest and reliable results. During the workshop, we’ll delve into a range of important topics, including what true deconvolution really means, how experimental settings influence the microscope PSF, the best ways to correct for noise and blur, how to identify and fix common acquisition issues, and advanced methods for visualizing and analyzing your data. A workshop manual, demo images, temporary Huygens Everywhere trial will be provided for this workshop.
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