Colocalization Analysis is a well-established method to quantify the extent to which fluorescent signal of two different objects or molecule populations are related. During this online lecture, we will discuss the influence of image quality on colocalization analysis, and how you can improve the fidelity of colocalization analysis by restoring imaging artifacts, as is also discussed in this article. This type of investigation will be aided by the recently launched Huygens Image Quality Control option, of which a free version is avaliable online via the Free Tools tab.
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There is a growing realization that Light Sheet image data benefits from advanced image restoration workflows that combine multiple tasks and can address any type of Light Sheet setup using a specific PSF model. The Huygens state-of-the-art PSF generator can model PSFs for any Light Sheet configuration and accounts for variations along the sheet direction, such as those seen in Gaussian Light Sheets. Tasks that can be included in a Light Sheet image restoration workflow include de-skewing, deconvolution, stitching, and colocalization or object-based analysis. In this live webinar, we will explain the various tools in Huygens that can be used to restore and analyze different types of Light Sheet data and offer you the opportunity to ask questions directly to our imaging specialists.
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