Huygens in scientific publications

Below is just a selection of articles that refer to the use of Huygens for different image processing tasks. For a more complete overview and for searching for specific applications, we recommend to search using for example Google Scholar. On this wiki page you find information on how to cite the use of Huygens.


Shinde A.P., Kučerová1 J., Dacks J.B. amd Tachezy J. (2024) Tracing back the expansion of the endomembrane rescue systems in parasitic Parabasalids to the ancestral origin in its free-living sister lineage. bioRxiv preprint
Huygens was used for deconvolution and calculating Pearson’s correlation coefficient (PCC/R) using Costes methodcolocalization analysis.

Iyer, R.S., Needham, S.R., Galdadas, I. et al. (2024) Drug-resistant EGFR mutations promote lung cancer by stabilizing interfaces in ligand-free kinase-active EGFR oligomers. Nat Commun 15, 2130.
Colocalization analyses were carried out the Huygens Software

Appelman, B., Charlton, B.T., Goulding, R.P. et al. (2024) Muscle abnormalities worsen after post-exertional malaise in long COVID. Nature Commun. 15, 17
Huygens was used for deconvolution of images acquired with the Abberior STED Expert Line.

Farkas D., Szikora S., Jijumon A.S., Polgár T.F., Patai R., Tóth M.Á. et al. (2024) Peripheral thickening of the sarcomeres and pointed end elongation of the thin filaments are both promoted by SALS and its formin interaction partners. PLoS Genet 20(1): e1011117
Huygens Deconvolution and visualization with the Surface Render was applied for generating 3D images to demonstrate the complex spatial structure of the giant Z-discs.

Elhassan E.A.E, Kmochová T., Benson K.A. et al. (2024) A Novel Monoallelic ALG5 Variant Causing Late-onset ADPKD and Tubulointerstitial Fibrosis. Kidney International Reports,

The Pearson coefficients of the colocalization and the colocalization maps were created on deconvolved images with the use of Huygens Professional Software.

Delling, J.P., Bauer, H.F., Gerlach-Arbeiter, S. et al. (2024) Combined expansion and STED microscopy reveals altered fingerprints of postsynaptic nanostructure across brain regions in ASD-related SHANK3-deficiency. Mol Psychiatry, April 22, 2024
Huygens was used for image deconvolution stabilization of confocal and STED data.

Van der Gaag B.L., Deshayes N.A.C., Breve J.J.P. et al. (2024) Distinct tau and alpha-synuclein molecular signatures in Alzheimer’s disease with and without Lewy bodies and Parkinson’s disease with dementia. Acta Neuropathol 147, 14.
Huygens was used for bleaching correction and deconvolution.

Radkohl A., Schusterbauer V., Bernauer L., Rechberger G.N., Wolinski H., Schittmayer M., Birner-Gruenberger R., Thallinger G.G., Leitner E., Baeck M. et al. (2024) Human Sterols Are Overproduced, Stored and Excreted in Yeasts. International Journal of Molecular Sciences. 25(2):781
Huygens was used for deconvolution.

López-Alcalá, J., Gordon, A., Trávez, A. et al. (2024) Localization, traffic and function of Rab34 in adipocyte lipid and endocrine functions. J Biomed Sci 31, 2
Huygens was used for deconvolving Zeiss LSM710 confocal images.

Battison A.S., Liddle J.C., Sumsky S.L., O’Connell C.B., Balsbaugh J.L. and LoTurco J.J. (2024) Synaptic Proteomes of Cortical Interneuron Classes Revealed by Antibody Directed Proximity Labeling. bioRxiv 2024.01.03.574066
Huygens was used for deconvolving images acquired with the Abberior STED Expert Line.

Eggelbusch et al., 2024) The impact of bed rest on human skeletal muscle
metabolism. Cell Reports Medicine 5, 101372
Huygens was used for deconvolving Zeiss Axiovert 200M widefield images.

Scutigliani, E.M., van Hattum, J., Lobo-Cerna, F., Kruyswijk, J., Myrcha, M., Dekkers, F.E.G.A., Hoebe, R.A., Edwards, F., Oppelaar, J.J., Vogt, L. et al. (2024) Perturbation of Copper Homeostasis Sensitizes Cancer Cells to Elevated Temperature. Int. J. Mol. Sci. 2024, 25, 423.
Huygens was used for deconvolving of images acquired with a Leica DM6i microscope.


Awoniyi et al. (2023). B cell receptor-induced protein dynamics and the emerging role of
SUMOylation revealed by proximity proteomics. Journal of Cell Science, 2023.
Huygens was used for deconvolution, for 3D particle analysis, and for colocalization measurements

Lei et al. (2023). Cooperative sensing of mitochondrial DNA by ZBP1 and cGAS promotes cardiotoxicity. Cell. 186,14: P3013-3032 E22.
Huygens Essential was used for deconvolving images

Antunes, A.V., Shahinas, M., Swale, C. et al. (2023). In vitro production of cat-restricted Toxoplasma pre-sexual stages. Nature. Nature 2023.
Huygens Professional was used for deconvolving confocal images

Malachowski et al. (2023). Spatially coordinated heterochromatinization Cell 186, 5840–5858.
DNA FISH images were deconvolved with Huygens Essential using the Classic MLE algorithm

Drees l, Schneider S, Riedel D, Schuh R and M Behr. (2023). The proteolysis of ZP proteins is essential to control cell membrane structure and integrity of developing tracheal tubes in Drosophila. Journal of Cell Science, 2023.
Zeiss Airyscan data was deconvolved with Huygens

Xie et al. (2023). Opposite changes in the expression of clathrin and caveolin-1 in normal and cancerous human prostate tissue: putative clathrin-mediated recycling of EGFR. Histochem Cell Biol 159, 489–500.
Deconvolution and colocalization analysis was performed with Huygens

Scholaert et al. (2023). 3D deconvolution of human skin immune architecture with Multiplex Annotated Tissue Imaging System. Science Advances vol 9, No 23.
Huygens was used for deconvolution, and also for crosstalk correction to address spectral spillover often seen with autofluorescence and imaging multiple fluorophores

Fukuda et al. (2023). The mitochondrial intermembrane space protein mitofissin drives mitochondrial fission required for mitophagy. Molecular Cell 83, 2045–2058.
Huygens Professional was used for deconvolving Zeiss Airyscan images

Runsala M, Kuokkanen E, Uski E, Šuštar V, Balci MÖ, Rajala J, Paavola V, Mattila PK. (2023). he Small GTPase Rab7 Regulates Antigen Processing in B Cells in a Possible Interplay with Autophagy Machinery. Cells. 12(21):2566.
Huygens was used for deconvolution and colocalization analysis using Manders overlap coefficient, Pearson’s correlation coefficient, and the overlap coefficient

Malachowski T. et al. (2023). Article
Spatially coordinated heterochromatinization of long synaptic genes in fragile X syndrome. Cell 186, 5840–5858.
Huygens Essential was used for deconvolution

Grochow et al. (2023). Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii. Communications Biology 6:1209.
Huygens Professional was used for deconvolving CLSM image to reduce optical aberrations and to correct for the discrepancy in refractive indices (IR) of the embedding (IR = 1.49)

Eriksson I, Vainikka L, Wäster P, Öllinger K. (2023). Lysosomal Function and Intracellular Position Determinethe Malignant Phenotype in Malignant Melanoma. Journal of Investigative Dermatology (2023).
Images were deconvolved, and subsequently analyzed with Huygens Object Analyzer and Object Tracker to determine lyosomal size, position and speed

Hassan A, Chandra V, Taleb S, Habushi S, Patzek T (2023). 3D confocal imaging methodology optimized for pore space characterization of carbonates. Engineering Geology, 2023.
Huygens deconvolution increased reliabilty of pore measurements in Carbonate, and Huygens was used for PSF quality control measurements (experimental vs theoretical PSF).

Makai D, Mihok E, Polgari D, Cseh A, Lenyko-Thegze A, Sepsi A, Sagi L. (2023). A unique mRNA decapping complex in trypanosomes. Plant Methods 19:80.
Huygens deconvolution is used as part of a newly developed procedure to prepare plant cell nuclei for high quality 3D immunolabelling‑in situ hybridisation (FISH)

Kramer et al. (2023). A unique mRNA decapping complex in trypanosomes. Nucleic Acids Research, 2023.
Huygens was used for deconvolution

Joensuu et al. (2023). Presynaptic targeting of botulinum neurotoxin type A requires a tripartite PSG-Syt1-SV2 plasma membrane nanocluster for synaptic vesicle entry. The EMBO Journal (2023)42.
Huygens was used for deconvolution

Huang A, Adler J, Parmryd I (2023). Optimised generalized polarisation analysis of C-laurdan reveals clear order
differences between T cell membrane compartments. BBA - Biomembranes 1865 (2023) 184094.
This article describes how deconvolution improves both the resolution and precision of GP-measurement and will enable detection of smaller changes in membrane order

Tammineni E.R, Figueroa L, Manno C, Varma D, Kraeva N, Ibarra C.A, Klip A, Riazi S, Rios E. (2023). Muscle calcium stress cleaves junctophilin1, unleashing a gene regulatory program predicted to correct glucose dysregulation. eLife 12:e78874..
The point spread function (PSF) of the system, deconvolution, and 3D ‘Simulated Fluorescence Process’ rendering was performed with Huygens Professional

Hellani F., Leleu I., Saidi N., Martin N., Lecoeur C., Werkmeister E., Koffi D., Trottein F., Yapo-Etté H., Das B., Abbadie C. and Pied S. (2023). Role of astrocyte senescence regulated by the non– canonical autophagy in the neuroinflammation associated to cerebral malaria. Brain, Behavior, and Immunity, Volume 117, Pages 20-35.
Images from the Zeiss AxioScan.Z1 slide scanner were improved using Huygens deconvolution


Auguste Y.S.S, Ferro A, Kahng J.A, Xavier A.M, Dixon J.R, Vrudhula U, Nichitiu A-S, Rosado D, Wee T-L, Pedmale U.V, Cheadle L. (Dec 2022). Oligodendrocyte precursor cells engulf synapses during circuit remodeling in mice. Nat Neurosci 25, 1273–1278 (2022)
Huygens deconvolution, crosstalk correction, and drift correction was used.

Rodriguez-Gatica JE, Iefremova V, Sokhranyaeva L, Au Yeung SWC, Breitkreuz Y, Bruestle O, Schwarz MK, Kubitscheck U. (June 2022). Imaging three-dimensional brain organoid architecture from meso- to nanoscale across development
Development (2022) 149 (20): dev200439
Image stacks of organoids were deconvolved with Huygens using theoretical point spread functions (PSF), based on microscopic parameters, or a measured PSF determined by analysis of fluorescent microbeads.

Räsänen N, Harju V, Joki, Narkilahti S. (June 2022). Practical guide for preparation, computational reconstruction and analysis of 3D human neuronal networks in control and ischaemic conditions. Development (2022) 149 (20): dev200439.
After imaging, the 3D stacks were deconvolved with Huygens Essentials software.

Vaisey G, Banerjee P, North A.J, Haselwandter C.A, Mackinnon R. (Dec 2022). Piezo1 as a force-through-membrane sensor in red blood cells. eLife 11:e82621
Only with Huygens deconvolution of STED data, the authors could reveal Piezo1 distribution on the RBC membrane, and resolve single Piezo1 channels.

Koornneef L, Slotman J.A, Sleddens-Linkels E, van Cappellen W.A., Barchi M, Toth A, Gribnau J, Houtsmuller A, Baarends W.M. (July 2022). Multi-color DSTORM microscopy in Hormad 1-/- spermatocytes reveals alternations in meiotic recombination intermediates and synaptonemal complex structure. PLoS Genet 18(7)
This paper describes an so far undiscovered phenotype of the SC in Horman 1-/- spermatocytes. Huygens Localizer was used for the imaging and analysis of the 3D dSTORM images.

Rodrigues-Oliveira T, Wollweber F, Ponce-Toledo RI, Xu J, Rittmann SK, Klingl A, Pilhofer M, Schleper C. (2022). Actin cytoskeleton and complex cell architecture in an Asgard archaeon.Nature (2022) 1-8
Huygens was used for deconvolution of STED and Airyscan images

Raykova D, Kermpatsou D, Malmqvist T, Harrison P.J., Rubin Sander M, Stiller C, Heldin J, Leino M, Ricardo S, Klemm A, David L, Spjuth O, Vemuri K, Dimberg A, Sundqvist A, Norlin M, Klaesson A,
Kampf C, & Söderberg O. (2022). A method for Boolean analysis of protein interactions at a molecular level. Nature Communications 13:4755
Huygens was used to deconvolve widefield images

Tortosa E, Sengupta Ghosh A, Li Q, Ruh Wong W, Hinkle T, Sandoval W, Rose C.M, Hoogenraad C.C. (2022). Stress-induced vesicular assemblies of dual leucine zipper kinase are signaling hubs involved in kinase activation and neurodegeneration. The EMBO Journal (2022)41:e110155
Huygens was used for deconvolution of confocal images

Corrêa de Melo T, Trevisan-Silva D, Alvarez-Flores M.P, Nascimento Gomes R, Medina de Souza M, Paula Valerio H, Oliveira D.S, DeOcesano-Pereira C, Fongaro Botosso V, Attie Calil Jorge S, Schattner M, Gomez R.M, Chudzinski-Tavassi A.M. (2022). Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells. MDPI (2022)
Huygens was used for the deconvolution and object analysis of confocal images

Fourriere L, Cho E.H-J, Gleeson P.A. (2022). Segregation of the membrane cargoes, BACE1 and amyloid precursor protein (APP) throughout the Golgi apparatus. Traffic Vol 23:3,158-173)
This paper shows a comparision of Huygens deconvolution with Zenn processing of Airyscan1 data processing. Huygens offers a 90nm lateral resolution

Traynor S, Jakobsen N.D, Ebbesen M.F, Bennedsen S.N, Johansen S, Ebstrup M.L, Pedersen C.B, Ditzel H.J, Brewer J.R, Gjerstorff M.F. (2022). SSX2 promotes the formation of a novel type of intranuclear lamin bodies. Int. J. Biochem. Cell Biol. (2022)
Deconvolution of Abberior Facility Line STED images

Bonilla, J.C, Clausen, M.P. (2022). Super-resolution microscopy to visualize and quantify protein microstructural organization in food materials and its relation to rheology: Egg white proteins. Food Hydrocolloids (2022)
Deconvolution of Abberior Facility Line STED images

Ma D, Wang F, Wang R, Hu Y, Chen Z, Huang N, Tian Y, Xia Y, Teng J, Chen J. (2022). α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization. Elife (2022)
Deconvolution of STEDYCON and Leica TCS SP8 STED 3X images.

Mazuelas H, Magallón-Lorenz M, Fernández-Rodríguez J, Uriarte-Arrazola I, Richaud-Patin Y, Terribas E, Villanueva A, Castellanos E, Blanco I, Raya Á, Chojnacki, J, Heyn H, Romagosa C, Lázaro C, Gel B, Carrió M, Serra E. (2022). Modeling iPSC-derived human neurofibroma-like tumors in mice uncovers the heterogeneity of Schwann cells within plexiform neurofibromas. Cell Reports (2022)
Stedycon images where stitched and deconvolved with Huygens


Moore A.S, Coscia S.M, Simpson C.L, Ortega F.E, Wait E.C, Heddleston J.M, Nirschl J.J, Obara C.J, Guedes-Dias P. Boecker C.A, Chew T-L, Theriot J.A, Lippincott-Schwartz J, Holzbaur E.L.F. (2021). Actin cables and comet tails organize
mitochondrial networks in mitosis. Nature (2021)
Spinning disk images were deconvolved with Huygens.

Gerasimaitė R, Bucevičius J, Kiszka K.A, Schnorrenberg S, Kostiuk G, Koenen T, Lukinavičius G (2021).
Blinking Fluorescent Probes for Tubulin Nanoscopy in Living and Fixed Cells. ACS Chemical Biology 2021 16 (11), 2130-2136
SMLM images from a Spinning disk/TIRF/SMLM system were analyzed with Huygens Localizer.

Noureddine A, Paffett M.L, Franco S.F, Chan A.E, Pallikkuth S, Lidke K, Serda R.E, (2021). Endolysosomal Mesoporous Silica Nanoparticle Trafficking along Microtubular Highways. Pharmaceutics 2022, 14(1), 56
Spinning disk images were deconvolved with Huygens.

Zamakhaeva S, Chaton C.T, Rush J.S, Castro S.A, Kenner C.W, Yarawsky A.E, Herr A.B, van Sorge N.M, Dorfmueller H.C, Frolenkov G.I, Korotkov K.V, Korotkova N. (2021). Modification of cell wall polysaccharide guides cell
division in Streptococcus mutans. Nature Chemical Biology. May 27
Images were deconvolved with Huygens.

Walker C.K, Greathouse K.M, Boros B.D, Poovey E/H, Clearman K.R, Ramdas R, Muhammad H.M, Herskowitz J.H. (2021). Dendritic Spine Remodeling and Synaptic Tau Levels in PS19 Tauopathy Mice. Neuroscience
Volume 455, 10 February 2021, Pages 195-211

Confocal stacks of dendrites were deconvolved using Huygens Professional.

Wu Y, Wang N, Song X, Cong S, Zhao X, Tan M. (2021). Fluorescence nanoparticles from instant coffee accumulated in lysosome and induced lysosome-dependent cell death via necroptosis-like pathway. NanoImpact. vol. 21, January 2021, 100290

Fontana D, Garay E, Cevera L, Kratje R, Prieto C, Gòdia F. (2021). Chimeric VLPs Based on HIV-1 Gag and a Fusion Rabies Glycoprotein Induce Specific Antibodies against Rabies and Foot-and-Mouth Disease Virus. Vaccines 2021, 9(3), 251

Dumbovića G, Sanjuan X, Perucho M, Forcales S-V. (2021). Stimulated emission depletion (STED) super resolution imaging of RNA- and protein-containing domains in fixed cells. Methods. Volume 187, March 2021, Pages 68-76
Huygens was used for deconvolution and PSF Distiller for for extracting experimental PSFs.

Oliveira L.S, Sumera A, Booker S.A. (2021). Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling. STAR Protocols
Volume 2, Issue 1, 19 March 2021, 100336

Ventura J.A, Donoghue J.F, Nowell C.J, Cann L.M, Day L.R.J, Smyth L.M.L, Forrester H.B, Rogers P.A.W, Crosbie J.C. (2021). The γH2AX DSB marker may not be a suitable biodosimeter to measure the biological MRT valley dose. International Journal of Radiation Biology

Zorkau M, Proctor-Kent Y, Berlinguer-Palmini R, Hamilton A, Chrzanowska-Lightowlers Z.M, Lightowlers R.N. (2021). Visualizing Mitochondrial Ribosomal RNA and Mitochondrial Protein Synthesis in Human Cell Lines. Mitochondrial Gene Expression. Methods in Molecular Biology, vol 2192. Humana, New York, NY.

Champroux A, Goubely C, Henry-Berger J, Drevet J.R, Kocer A. (2021). Three-Dimensional Confocal Analysis of Chromosome Positioning Coupled with Immunofluorescence in Mouse Sperm Nuclei. In: Ruzov A., Gering M. (eds) DNA Modifications. Methods in Molecular Biology, vol 2198. Humana, New York, NY.

Lucidi M, Hristu R, Nichele L, Stanciu G.A, Visca P, Banica C.K, Cincotti G, Stanciu S.G. (2021). Characterization of Acinetobacter baumannii Filamentous Cells by Re-Scan Confocal Microscopy and Complementary Fluorometric Approaches. In IEEE Journal of Selected Topics in Quantum Electronics, vol. 27, no. 5, pp. 1-7, Sept.-Oct. 2021
Huygens was used to deconvolve both Confocal and RCM (Rescan) images.

Aicher S-M, Monaghan P, Netherton CL, Hawes PC. (2021). Unpicking the Secrets of African Swine Fever Viral Replication Sites. Viruses. 2021; 13(1):77.

Jähne S, Mikulasch F, Heuer H.G.H, Truckenbrodt S, Agüi-Gonzalez P, Grewe K, Vogts A, Rizzoli S.O, Priesemann V. (2021). Presynaptic activity and protein turnover are correlated at the single-synapse level. Cell Reports. volume 34, Issue 11, 16 March 2021


Jonkman J, Brown CM, Wright GD, Anderson KI, North AJ. (2020). Tutorial: guidance for quantitative confocal microscopy.
Nature Protocols - review Mar 31 2020
This paper refers to Huygens as the deconvolution package that can address many different microscopy images. The authors also emphasize the importance of preserving quantitative information by treating images similarly. Huygens unique Workflow Proccesor and Huygens Remote Manager are ideal for this purpose.

Willems J, de Jong A ,Scheefhals N, Mertens E, Catsburg L, Poorthuis R, de Winter F, Verhaagen J, Meye F, MacGillavry H (2020). ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons
PLOS Biology April 10, 2020
In vivo STED imaged of dendrites were deconvolved with Huygens.

Bucevičius J, Gilat T, Lukinavičius G. (2020). Far-red switching DNA probes for live cell nanoscopy
Chem. Commun., 2020, 56, 14797-14800
Huygens Localizer was used to analyze SMLM images.

Hildebrand, S., Schueth, A., Wangenheim, K.v. et al. (2020). hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue.
Sci Rep 10, 9950
Huygens PSF distiller, Object Stabilizer for deskewing, and deconvolution was used for restoring Light Sheet data.

González-Domínguez I, Puente-Massaguer E, Cervera L, Gòdia F. (2020). Quality Assessment of Virus-Like Particles at Single Particle Level: A Comparative Study.
Viruses 2020, 12(2), 223
Huygens was used for deconvolving Leica SP8 confocal data.

González-Domínguez I, Puente-Massaguer E, Cervera L, Gòdia F. (2020). Quantification of the HIV‐1 virus‐like particle production process by super‐resolution imaging: from VLP budding to nanoparticle analysis.
Biotechnology and Bioengineering. April 2020
Huygens was used for deconvolving Leica SP8 confocal data.

Gӧbel J, Engelhardt E, Pelzer P, et al. Mitochondria-Endoplasmic Reticulum Contacts in Reactive Astrocytes Promote Vascular Remodeling.
Cell Metab. 2020, 31(4): 791–808
Huygens deconvolution and 3D object analysis was used for measuring mitochondrial morphology parameters (length, voxel volume and sphericity).

Aoyama-Ishiwatari S, Okazaki T, Iemura S-i, Natsume T, Okada Y, Gotoh Y. (2020). NUDT21 links mitochondrial IPS-1 to RLR-containing stress granules and 3 activates host antiviral defense.
bioRxiv, preprint April 2020
Huygens was used for deconvolution and 3D object counting.

Bolte S., Marcon E., Jaunario M., Moyet L., Paternostre M., Kuntz M., Krieger-Liszkay A. (2020). Dynamics of the localization of the plastid terminal oxidase inside the chloroplast.
Journal of Experimental Botany, publ. Feb 15, 2020

Jarjour A.A., Velichkova A.N., Boyd A., Lord K.M., Torsney C., Henderson D.J., Ffrench-Constant C (2020). The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2
GLIA, publ. March 3, 2020
Huygens was used for deconvolving STED image data.

Page J., Saltarin F., Belyaev Y., Lyck R., Favaro P. (2020). Learning to Reconstruct Confocal Microscopy Stacks from Single Light Field Images.
Arxiv.org, publ. March 24, 2020
The CMLE algorithm was used with the Huygens Remote Manager, the web-based implementation of Huygens Core deconvolution software.

Kumar Kondadi A, Anand R, Hänsch S, Urbach J, Zobel T, Wolf DM, Segewa M, Liesa M, Shirihait OS, Weidtkamp‐Peters S, Reichert AS. (2020). Cristae undergo continuous cycles of membrane remodelling in a MICOS‐dependent manner.
EMBO Rep (2020)21:e49776
Quote: Due to the strong depletion and the drastic decrease in emitting fluorophores in STED microscopy, the resulting images tend to have a lower signal‐to‐noise ratio. The increase in axial resolution, and by that reduction in out of focus blur, is not the only benefit of deconvolution. The other major advantages are increase in signal‐to‐noise ratio and resolution.

Bello-Gamboa A, Velasco M, Moreno S, Herranz G, Ilie R, Huetos S, Dávila S, Sánchez A, Bernardino De La Serna J, Calvo V, Izquierdo M (2020). Actin reorganization at the centrosomal area and the immune synapse regulates polarized secretory traffic of multivesicular bodies in T lymphocytes.
J Extracell Vesicles (2020) Jun 19;9(1):1759926.
Widefield and confocal images were deconvolved with Huygens.


Gambarotto D, Zwettler FU, Le Guennec M, Schmidt-Cernohorska M, Fortun D, Borgers S, Heine J, Schloetel JG, Reuss M, Unser M, Boyden ES, Sauer M, Hamel V, Guichard P (2019) Imaging cellular ultrastructures using expansion microscopy (U-ExM)
Nat Methods. 2019 Jan; 16(1): 71–74
Huygens deconvolution used in combination with expansion microscopy

Hötte K, Koch M, Hof L, Tuppi M, Moreth T, Verstegen MMA, van der Laan LJW, Stelzer EHK, Pampaloni F. (2019).
Ultra-thin fluorocarbon foils optimise multiscale imaging of three-dimensional native and optically cleared specimens.
Sci Rep 9, 17292 (2019)
Huygens was used for Light Sheet FUSION

Bhukel, A., Beuschel, C. B., Maglione, M., Lehmann, M., Juhász, G., Madeo, F., & Sigrist, S. J. (2019). Autophagy within the mushroom body protects from synapse aging in a non-cell autonomous manner.
Nature communications, 10(1), 1318.
Huygens was used for deconvolution

Watanabe K, Takao D, Ito KK, Takahashi M, Kitagawa D (2019).
The Cep57-pericentrin module organizes PCM expansion and centriole engagement
Nature Communications vol10 (2019)
Huygens was used for deconvolution of STED and confocal image data

Düring D, Diales Rocha M, Dittrich F, Gahr M, Hahnloser R (2019). Expansion Light Sheet Microscopy Resolves Subcellular Structures in Large Portions of the Songbird Brain.
Front. Neuroanat., 31 January 2019
Huygens deconvolution was used in combination with expansion microscopy and light sheet imaging

Matczuk,AK, Chodaczek G, Ugorski M. (2019). Production of Recombinant EAV with Tagged Structural Protein Gp3 to Study Artervirus Minor Protein Localization in Infected Cells
Viruses. 2019 Aug; 11(8): 735.
Huygens was used for deconvolution

Spahn C, Grimm JB, Lavis LD, Lampe M, Heilemann M. (2019). Whole-Cell, 3D, and Multicolor STED Imaging with Exchangeable Fluorophores.
Nano Lett.;19(1):500-505.
Huygens was used for deconvolution

Bello-Gamboa, A., Izquierdo, J. M., Velasco, M., Moreno, S., Garrido, A., Meyers, L., Palomino, J. C., Calvo, V., Izquierdo, M. Imaging the Human Immunological Synapse. J. Vis. Exp. (154).
Huygens was used for deconvolution. A video journal available online at JoVE also shows the difference Huygens deconvolution makes.

Mohan S, Coto Hernandez I, Selig MK, Shibata S, Jowett N. (2019).
Stain-Free Resolution of Unmyelinated Axons in Transgenic Mice Using Fluorescence Microscopy.
J. Neuropathol Exp Neurol vol.0,no.0: 1-3
Huygens was used for deconvolution and Remko Dijkstra (SVI) was acknowledged for guidance with the imaging

Lingemann M, McCarty T, Liu X, Buchholz UJ, Surman S, Martin SE, Collins PL, Munir S (2019).
The alpha-1 subunit of the Na+,K+-ATPase (ATP1A1) is required for macropinocytic entry of respiratory syncytial virus (RSV) in human respiratory epithelial cells.
Plos Pathogens, 05 Aug 2019
Huygens was used for deconvolution

Li, Y-F, Canário AVM, Power DM, Campinho MA. (2019). Ioxynil and diethylstilbestrol disrupt vascular and heart development in zebrafish. Environment International. 124, 511-520.Epub 2019 Jan 25.
Huygens was used for deconvolution

Fekete, A., Nakamura, Y., Yang, Y. M., Herlitze, S., Mark, M. D., DiGregorio, D. A., & Wang, L. Y. (2019). Underpinning heterogeneity in synaptic transmission by presynaptic ensembles of distinct morphological modules.
Nature communications, 10(1), 826.
Huygens was used for deconvolution

Bommel B, Konietzny A, Kobler O, Bär J and Mikhaylova M. (2019). Anisotropic mechanics and dynamics of a living mammalian cytoplasm. EMBO J 38:e101183, 15(2), 190-199
Huygens was used for confocal and STED deconvolution

Moran KD, Kang H, Araujo AV, Zyla TR, Saito K, Tsygankov D, Lew DJ. Cell-cycle control of cell polarity in yeast.
J Cell Biol (2019) 218 (1): 171-189.
Huygens was used for confocal and STED deconvolution

Gupta, S. K., Li, Y., & Guo, M. (2019). F‐actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes. Soft matter, 15(2), 190-199.
Huygens was used for deconvolution

Butler MB, Short NE, Maniou E, Alexandre P, Greene NDE, Copp AJ, Galea GL. (2019). Rho kinase-dependent apical constriction counteracts M-phase apical expansion to enable mouse neural tube closure. Journal of Cell Science 2019 132.
Huygens was used for deconvolution

Russell MA, Redick SD, Blodgett DM, Richardson SJ, Leete P, Krogvold L, Dahl-Jørgensen K, Bottino R, Brissova M, Spaeth JM, Babon JAB, Haliyur R, Powers AC, Yang C, Kent SC, Derr AG, Kucukural A, Garber MG, Morgan NG and Harlan DM (2019). HLA Class II Antigen Processing and Presentation Pathway Components Demonstrated by Transcriptome and Protein Analyses of Islet β-Cells From Donors With Type 1 Diabetes. Diabetes May; 68(5): 988-1001.15.
Huygens was used for deconvolution

Berndt-Paetz M, Herbst L, Weimann A, Gonsior A, Stolzenburg JU, Neuhaus J. (2019). IC/BPS‐associated alterations of M2 and M3 muscarinic acetylcholine receptor trafficking in human detrusor.
Neurourology & Urodynamics 2019; 1-10.
Huygens was used for deconvolution, colocalization, and object analysis

Foidl, B. M., & Humpel, C. (2019). Chronic treatment with five vascular risk factors causes cerebral amyloid angiopathy but no Alzheimer pathology in C57BL6 mice.
Brain, behavior, and immunity.
Huygens was used for deconvolution

Baharlou, H., Canete, N. P., Bertram, K. M., Sandgren, K. J., Cunningham, A. L., Harman, A. N., & Patrick, E. (2019). Digital removal of autofluorescence from microscopy images. BioRxiv, 566315.
BioRxiv, 566315
Huygens was used for deconvolution

Bartolomé-Martín, D., Ibáñez, I., Piniella, D., Martínez-Blanco, E., Pelaz, S. G., & Zafra, F. (2019). Identification of potassium channel proteins Kv7. 2/7.3 as common partners of the dopamine and glutamate transporters DAT and GLT-1. .
Neuropharmacology, March 2019
Huygens was used for deconvolution

Bentivoglio, M., Cotrufo, T., Ferrari, S., Tesoriero, C., Mariotto, S., Bertini, G., ... & Mazzarello, P. (2019). The original histological slides of Camillo Golgi and his discoveries on neuronal structure..
Frontiers in Neuroanatomy, 13
Huygens was used for deconvolution

Sauer, F., Oswald, L., de Schellenberger, A. A., Tzschätzsch, H., Schrank, F., Fischer, T., ... & Käs, J. A. (2019). Collagen networks determine viscoelastic properties of connective tissues yet do not hinder diffusion of the aqueous solvent.
Soft Matter 2019, 15, 3055-3064
Huygens was used for deconvolution

Kelley, J. B., & Paschal, B. M. (2019). Fluorescence-based quantification of nucleocytoplasmic transport. Methods, 157, 106-114.
Methods Methods 157:15, Pages 106-114
Huygens was used for deconvolution

Pandey, G., Westhoff, J. H., Schaefer, F., & Gehrig, J. (2019). A Smart Imaging Workflow for Organ-Specific Screening in a Cystic Kidney Zebrafish Disease Model. International journal of molecular sciences, 20(6), 1290.
Huygens was used for deconvolution

Bürgers, J., Pavlova, I., Rodriguez-Gatica, J. E., Henneberger, C., Oeller, M., Ruland, J. A., ... & Schwarz, M. K. (2019). Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution. Neurophotonics, 6(1), 015005.
Huygens was used for deconvolution

Velazquez, F. N., Miretti, M., Baumgartner, M. T., Caputto, B. L., Tempesti, T. C., & Prucca, C. G. (2019). Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study.
Scientific reports, 9(1), 3010.
Huygens was used for deconvolution

Souriant, S., Balboa, L., Dupont, M., Pingris, K., Kviatcovsky, D., Cougoule, C., ... & Raynaud-Messina, B. (2019). Tuberculosis Exacerbates HIV-1 Infection through IL-10/STAT3-Dependent Tunneling Nanotube Formation in Macrophages.
Cell Reports, 26(13), 3586-3599.
Huygens was used for deconvolution

Fekete, A., Nakamura, Y., Yang, Y. M., Herlitze, S., Mark, M. D., DiGregorio, D. A., & Wang, L. Y. (2019). Underpinning heterogeneity in synaptic transmission by presynaptic ensembles of distinct morphological modules.
Nature communications, 10(1), 826.
Huygens was used for deconvolution

Hoette, K., Koch, M., Hof, L., Tuppi, M., Moreth, T., Stelzer, E. H., & Pampaloni, F. (2019). Ultra-thin fluorocarbon foils optimize multiscale imaging of three-dimensional native and optically cleared specimens. bioRxiv, 533844.
Huygens was used for deconvolution

Bucevičius, J., Keller-Findeisen, J., Gilat, T., Hell, S. W., & Lukinavičius, G. (2019). Rhodamine–Hoechst positional isomers for highly efficient staining of heterochromatin. Chemical Science.
Huygens was used for deconvolution

Colin-York, H., Li, D., Korobchevskaya, K., Chang, V. T., Betzig, E., Eggeling, C., & Fritzsche, M. (2019). Cytoskeletal actin patterns shape mast cell activation. Communications Biology, 2(1), 93.
Huygens was used for STED deconvolution

Gao, G., Zhu, C., Liu, E., & Nabi, I. R. (2019). Reticulon and CLIMP-63 control nanodomain organization of peripheral ER tubules.
BioRxiv, 550715.
Huygens was used for deconvolution

Fernandez-Chamorro J, Francisco-Velilla R, Ramajo J, Martinez-Salas E. (2019). Rab1b and ARF5 are novel RNA-binding proteins involved in FMDV IRES–driven RNA localization
Life Science Alliance Jan 2019, 2 (1)
Huygens was used for deconvolution

Gulvady, A. C., Forsythe, I. J., & Turner, C. E. (2019). Hic-5 regulates Src-induced invadopodia rosette formation and organization.
Molecular Biology of the Cell.
Huygens was used for deconvolution

Stewart DJ, Short KK, Maniaci BN, Burkhead JL (2019). COMMD1 and PtdIns(4,5)P2 interaction maintain ATP7B copper transporter trafficking fidelity in HepG2 cells.
Journal of Cell Science 132: jcs231753.
This study describes in detail the use of Huygens Colocalization Analyzer


Spiess M., Hernandez‐Varas P., Oddone A., Olofsson H., Blom H., Waithe D., Lock J.G., Lakadamyali M., Strömblad S., (2018). Active and inactive β1 integrins segregate into distinct nanoclusters in focal adhesions.
Journal of Cell Biol. 217(6):1929-1940.
Huygens was used for deconvolution of STED data

Hartveit E., Zandt B-J., Madsen E., Castilho A., Mørkve S.H., Veruki M.L., (2018). AMPA receptors at ribbon synapses in the mammalian retina: kinetic models and molecular identity. Brain Struct Funct. 2018 Mar;223(2):769-804.
Huygens was used for deconvolution, and the Huygens Object Analyzer was used to determine "the spatial relationships between fluorescent structures corresponding to (dye-filled) AII amacrine cell and immunolabeled punctae."

Chhatbar C, Detje CN. et al. (2018). Type I Interferon Receptor Signaling of Neurons and Astrocytes Regulates Microglia Activation during Viral Encephalitis.
Cell Report s 25, 118–129
Huygens was used for deconvolution and generating Maximum Intensity Projections of LSM 780images

Truckenbrodt S, Maidorn M, Crzan D, Wildhagen H, Kabatas S, Rizzoli SO. (2018). X10 expansion microscopy enables 25‐nm resolution on conventional microscopes
EMBO Rep (2018)19:e45836
Huygens deconvolution used in combination with expansion microscopy

Xiao, X., Djurisic, M., Hoogi, A., Sapp, R. W., Shatz, C. J., & Rubin, D. L. (2018). Automated dendritic spine detection using convolutional neural networks on maximum intensity projected microscopic volumes.
Journal of neuroscience methods, 309, 25-34.
Huygens was used for deconvolution

Karelehto E, Cristella C, Yu X, Sridhar A, Hulsdouw R, de Haan K, van Eijk H, Koekkoek S, Pajkrt D, de Jong MD, Wolthers KC (2018). Polarized Entry of Human Parechoviruses in the Airway Epithelium
Front Cell Infect Microbiol. 2018; 8: 294.
Huygens was used for deconvolution of confocal images

Milewska A, Nowak P, Owczarek K, Szczepanski A, Zarebski M, Hoang A, Berniak K, Wojarski J, Zeglen S, Baster Z, Rajfur Z, Pyrc K (2018). Entry of Human Coronavirus NL63 into the Cell..
Journal of Virology, 17 Jan 2018, 92(3)
Huygens was used for deconvolution of both Zeiss and Leica confocal images

Yue Y, Nabar NR, Shi C-S, Kamenyeva O, Xiao X, Hwang I-Y, Wang M, Kehrl JH. (2018). SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death.
Cell Death Dis. 2018 Sep; 9(9): 904.
Huygens was used for deconvolution of confocal images

Munganyinka E, Margaria P, Sheat S, Ateka EM, Tairo F, Ndunguru J, Winter S. Localization of cassava brown streak virus in Nicotiana rustica and cassava Manihot esculenta (Crantz) using RNAscope® in situ hybridization.
Virology Journal vol 15 (2018).
Huygens was used for deconvolution of confocal images

Ravikumar R, Kalbfuß N, Gendre D, Steiner A, Altmann M, Altmann S, Rybak K, Edelmann H, Stephan F, Lampe M, Facher E, Wanner G, Falter-Braun P, Bhalerao RP and Assaad FF. Independent yet overlapping pathways ensure the robustness and responsiveness of trans-Golgi network functions in Arabidopsis
Development 2018 145.
Huygens was used for deconvolution

Xiao, X., Djurisic, M., Hoogi, A., Sapp, R. W., Shatz, C. J., & Rubin, D. L. (2018). Automated dendritic spine detection using convolutional neural networks on maximum intensity projected microscopic volumes.
Journal of Neuroscience Methods, 309, 25-34.
Huygens was used for deconvolution

Gagliardi PA, Somale D, Puliafito A, Chiaverina G, di Blasio L. (2018). MRCKα is activated by caspase cleavage to assemble an apical actin ring for epithelial cell extrusion.
J Cell Biol (2018) 217 (1): 231-249.
Huygens was used for deconvolution

Calvo V., Izquierdo M. (2018). Imaging Polarized Secretory Traffic at the Immune Synapse in Living T Lymphocytes.
Front. Immunology 9:684.
This paper describes different microscopy techniques for studying the immunological secretory response and the importance of using Huygens deconvolution

Berndt-Paetz M, Herbst L, Weimann A, Gonsior A, Stolzenburg JU, Neuhaus J., (2018). Highly specific detection of muscarinic M3 receptor, G protein interaction and intracellular trafficking in human detrusor using Proximity Ligation Assay (PLA).
Acta Histochemica . 120:329-339.
Huygens was used for deconvolution, and colocalization and object analysis

Vibha Singh, Chaitanya Erady, Nagaraj Balasubramanian, Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells. J Cell Sci July 27, 2018.
Huygens was used for deconvolution; Golgi organization was analyzed with Huygens Colocalization Analyzer and Huygens Object Analyzer

Manso, Y., Holland, P. R., Kitamura, A., Szymkowiak, S., Duncombe, J., Hennessy, E., ... & McColl, B. W. (2018). Minocycline reduces microgliosis and improves subcortical white matter function in a model of cerebral vascular disease.
Glia, 66(1), 34-46.
Huygens was used for deconvolution.

Tuncay, E., Bitirim, V., Olgar, Y., Durak, A., Rutter, G. A., & Turan, B. (2018). Zn 2+-transporters ZIP7 and ZnT7 play important role in progression of cardiac dysfunction via affecting sarco (endo) plasmic reticulum-mitochondria coupling in hyperglycemic cardiomyocytes.
Mitochondrion 44: 41-52.
Huygens was used for deconvolution.

Malagnino, V., Hussner, J., Seibert, I., Stolzenburg, A., Sager, C. P., & zu Schwabedissen, H. E. M. (2018). LST-3TM12 is a member of the OATP1B family and a functional transporter. Biochemical pharmacology, 148, 75-87.
Huygens was used for deconvolution.

Markus M. Knodel, Arne Nägel, Sebastian Reiter, Andreas Vogel, Paul Targett-Adams, John McLauchlan, Eva Herrmann and Gabriel Wittum. Quantitative Analysis of Hepatitis C NS5A Viral Protein Dynamics on the ER Surface.
Viruses 2018, 10(1), 28
Huygens deconvolution was used for ER recontruction and SVI support was acknowledged.

Sugiyama, N., Sonay, A. Y., Tussiwand, R., Cohen, B. E., & Pantazis, P. (2018). Effective Labeling of Primary Somatic Stem Cells with BaTiO3 Nanocrystals for Second Harmonic Generation Imaging.
Small, 14(8).
Huygens was used for deconvolution.

Steiner, C., Gevaert, T., Ganzer, R., De Ridder, D., & Neuhaus, J. (2018). Comparative immunohistochemical characterization of interstitial cells in the urinary bladder of human, guinea pig and pig.
Histochemistry and cell biology, 1-11.
Huygens was used for deconvolution.

Zandt B.J., Veruki M.L., Hartveit E., (2018). Electrotonic signal processing in AII amacrine cells: compartmental models and passive membrane properties for a gap junction-coupled retinal neuron.
Brain Struct Funct. 2018 Sep;223(7):3383-3410.
Huygens was used for deconvolution, and the Object Stabilizer option was used to "align image slices along the Z-axis to compensate for drift and other mechanical instabilities."

Ashenafi, M. S., & Baroux, C. (2018). Automated 3D gene position analysis using a customized Imaris plugin: XTFISHInsideNucleus. In Plant Chromatin Dynamics (pp. 591-613). Humana Press, New York, NY.
Huygens was used for deconvolution.

Burdyniuk, M., Callegari, A., Mori, M., Nedelec, F., & Lenart, P. (2018). F-actin patches nucleated on chromosomes coordinate capture by microtubules in oocyte meiosis. bioRxiv, 265009.
Huygens was used for deconvolution.

Campinho MA., Silva, N., Martins GG., Anjos L., Florindo C., Roman-Padilla J., Garcia-Cegarra A., Louro B., Manchado M., Power DM, (2018). A thyroid hormone regulated asymmetric responsive centre is correlated with eye migration during flatfish metamorphosis. Scientific Reports. 8, 12267. August 16, 2018
Huygens was used for deconvolution.

Huang, X., Selewa, A., Wang, X., Daddysman, M. K., Gdor, I., Wilton, R., ... & Scherer, N. F. (2018). 3D Snapshot Microscopy of Extended Objects. arXiv preprint arXiv:1802.01565.
Huygens was used for deconvolution.


Fritzsche, M., Fernandes, R. A., Chang, V. T., Colin-York, H., Clausen, M. P., Felce, J. H., ... & Pedroza-Pacheco, I. (2017). Cytoskeletal actin dynamics shape a ramifying actin network underpinning immunological synapse formation. Science advances, 3(6), e1603032. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479650/
"Huygens was used for deconvolution of STED images"

Franklin TB, Silva BA, Perova Z, Marrone L, Masferrer ME, Zhan Y, Kaplan A, Greetham L, Verrechia V, Halman A, Pagella S, Vyssotski AL, Illarionova A, Grinevich V, Branco T, Gross CT (2017). Prefrontal cortical control of a brainstem social behavior circuit.
Nature Neuroscience, 09 Jan 2017, 20(2):260-270
''Huygens was used for deconvolution"

Baharom, F., Thomas O.S., Lepzien R., Mellman I., Chalouni C., Smed-Sörensen A. (2017). Visualization of early influenza A virus trafficking in human dendritic cells using STED microscopy. PLOS ONE (June 2017)
''Huygens was used for deconvolution of STED images and a reference was made to our STED article in Microscopy Today"

Freifeld L, Odstrcil I, Förster D, Ramirez A, Gagnon J, Randlett O, Costa E, Asano S, Celiker O, Gao R, Martin-Alarcon D, Reginato P, Dick C, Chen L, Schoppik D, Engert F, Baier H, Boyden E (2017). Expansion microscopy of zebrafish for neuroscience and developmental biology studies. PNAS December 12, 2017 114 (50) E10799-E10808
''Huygens deconvolution was used in combination with expansion microscopy"

Gil-Krzewska, A., Saeed, M. B., Oszmiana, A., Fischer, E. R., Lagrue, K., Gahl, W. A., ... & Krzewski, K. (2017). An actin cytoskeletal barrier inhibits lytic granule release from natural killer cells in patients with Chediak-Higashi syndrome. Journal of Allergy and Clinical Immunology.

Giraldo A.M.V., Öllinger K., Loitto V. (2017) Microscopic Analysis of Lysosomal Membrane Permeabilization. In: Öllinger K., Appelqvist H. (eds) Lysosomes. Methods in Molecular Biology, vol 1594. Humana Press, New York, NY DOI 10.1007/978-1-4939-6934-0_5

Arthurs C, Nazia Murtaza B, Thomson C, Dickens K,Henrique R, Patel HRH, Beltran M, Millar M, Thrasivoulou C and Ahmed A. (2017)
Expression of ribosomal proteins in normal and cancerous human prostate tissue. PLOS ONE, Oct 2017

Alex M. Valm, Sarah Cohen, Wesley R. Legant, Justin Melunis, Uri Hershberg, Eric Wait, Andrew R. Cohen, Michael W. Davidson, Eric Betzig & Jennifer Lippincott-Schwartz, Applying systems-level spectral imaging and analysis to reveal the organelle interactome, Nature 546, 162–167 (01 June 2017) doi:10.1038/nature22369

Sreejesh Sreedharan, Martin R. Gill, Esther Garcia, Hiwa K. Saeed, Darren Robinson, Aisling Byrne, Ashley Cadby, Tia E. Keyes , Carl Smythe, Patrina Pellett, Jorge Bernardino de la Serna, and Jim A. Thomas
Multimodal Super-resolution Optical Microscopy Using a Transition-Metal-Based Probe Provides Unprecedented Capabilities for Imaging Both Nuclear Chromatin and Mitochondria
J. Am. Chem. Soc., 2017, 139 (44), pp 15907–15913 DOI: 10.1021/jacs.7b08772, Publication Date (Web): October 4, 2017

Bury L, Coelho PA, Simeone A, Ferries S, Eyers CE, Eyers PA, Zernicka-Goetz M, Glover DM. (2017)
Plk4 and Aurora A cooperate in the initiation of acentriolar spindle assembly in mammalian oocytes Journal of Cell Biology

Cañas JA1,, Sastre B, Mazzeo C, Fernández-Nieto M, Rodrigo-Muñoz JM, González-Guerra A, Izquierdo M, Barranco P, Quirce S, Sastre J, Del Pozo V. (2017) Exosomes from eosinophils autoregulate and promote eosinophil functions Journal of Leukocyte Biology 101, 5: 1191-1199.

Carlo Manno, Lourdes C. Figueroa, Dirk Gillespie, Robert Fitts, ChulHee Kang, Clara Franzini-Armstrong, and Eduardo Rios.
Calsequestrin depolymerizes when calcium is depleted in the sarcoplasmic reticulum of working muscle PNAS, January 24, 2017
vol. 114 no. 4, E638–E647

Day, K. J., La Rivière, P. J., Chandler, T., Bindokas, V. P., Ferrier, N. J., & Glick, B. S. (2017). Improved deconvolution of very weak confocal signals. F1000Research, 6. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5553083.2/
This is a very interesting paper on how to improve extremely low signal data with Huygens.

Yang ASP, Lopaticki S, O'Neill MT, Erickson SM, Douglas DN, Kneteman NM, Boddey JA (2017). AMA1 and MAEBL are important for Plasmodium falciparum sporozoite infection of the liver.
Cellular Microbiology Vol 19, Issue9
Huygens was used for deconvolution

Song ZM, Bouchab L, Hudik E, Le Bars R, Nüsse O and Dupré‐Crochet S. Phosphoinositol 3‐phosphate acts as a timer for reactive oxygen species production in the phagosome. Journal of Leukocyte Biology Vol 101, 5.
Huygens was used for deconvolution

Day, K. J., La Rivière, P. J., Chandler, T., Bindokas, V. P., Ferrier, N. J., & Glick, B. S. (2017). Improved deconvolution of very weak confocal signals. Version 2. F1000Res. 2017; 6: 787.
This is a very interesting paper (note: read the included revision as well) on how to improve extremely low signal data with Huygens.


Nils J. Lambacher, Ange-Line Bruel, Teunis J. P. van Dam, Katarzyna Szymańska, Gisela G. Slaats, Stefanie Kuhns, Gavin J. McManus, Julie E. Kennedy, Karl Gaff, Ka Man Wu, Robin van der Lee, Lydie Burglen, Diane Doummar, Jean-Baptiste Rivière, Laurence Faivre, Tania Attié-Bitach, Sophie Saunier, Alistair Curd, Michelle Peckham, Rachel H. Giles, Colin A. Johnson, Martijn A. Huynen, Christel Thauvin-Robinet & Oliver E. Blacque,
TMEM107 recruits ciliopathy proteins to subdomains of the ciliary transition zone and causes Joubert syndrome Nature Cell Biology 18, 122–131 (2016) doi:10.1038/ncb3273

Xiaochao Tan, Priyam Banerjee, Hou-Fu Guo, Stephen Ireland, Daniela Pankova, Young-ho Ahn, Irodotos Michail Nikolaidis, Xin Liu, Yanbin Zhao, Yongming Xue, Alan R. Burns, Jonathon Roybal, Don L. Gibbons, Tomasz Zal, Chad J. Creighton, Daniel Ungar, Yanzhuang Wang, and Jonathan M. Kurie
Epithelial-to-mesenchymal transition drives a pro-metastatic Golgi compaction process through scaffolding protein PAQR11
J Clin Invest. 2017;127(1):117–131. doi:10.1172/JCI88736

Milewska A, Kaminski K, Ciejka J, Kosowicz K, Zeglen S, Wojarski J, Nowakowska M, Szczubiałka K, Pyrc K. (2017)
HTCC: Broad Range Inhibitor of Coronavirus Entry
PLoS One. 2016; 11(6).

Yulong Chen, Houfu Guo, Masahiko Terajima, Priyam Banerjee, Xin Liu, Jiang Yu, Amin A. Momin, Hiroyuki Katayama, Samir M. Hanash, Alan R. Burns, Gregg B. Fields, Mitsuo Yamauchi and Jonathan M. Kurie
Lysyl Hydroxylase 2 Is Secreted by Tumor Cells and Can Modify Collagen in the Extracellular Space
J. Biol. Chem. 2016 291: 25799-25808
The Huygens software was used to deconvolve and visualize the cover of this issue of The Journal of Biological Chemistry.

Zandt B-J, Liu J.H., Veruki M.L., Hartveit E.
AII amacrine cells: quantitative reconstruction and morphometric analysis of electrophysiologically identified cells in live rat retinal slices imaged with multi-photon excitation microscopy.
Brains Structure & Function 1–32 (2016)
This paper describes in much detail the optimimal imaging and deconvolutution settings that were applied.

Kochan J, Wawro M, Kasza A,
IF-combined smRNA FISH reveals interaction of MCPIP1 protein with IER3 mRNA
Biology Open 2016 : doi: 10.1242/bio.018010
Huygens was used for deconvolution and the Huygens Colocalization Analyzer option was applied].

Christian A. Ruge, Adam Bohr, Moritz Beck-Broichsitter, Valérie Nicolas, Nicolas Tsapis, Elias Fattal.
Disintegration of nano-embedded microparticles after deposition on mucus: a mechanistic study, Colloids and Surfaces B: Biointerfaces 139 (2016) 219–227

Barna L, Dudok B, Miczán V, Horváth A, I László Z, and Katona I
Correlated confocal and super-resolution imaging by VividSTORM.
Nature Protocols 11, 163–183 (2016)
Huygens deconvolution was used to create the cover of this issue of Nature Protocols.

Magalie Bénard, Damien Schapman, Alexis Lebon, Baptiste Monterroso, Marine Bellenger, Frank Le Foll, Jennifer Pasquier, Hubert Vaudry, David Vaudry and Ludovic Galas.
Structural and functional analysis of tunneling nanotubes (TnTs) using gCW STED and gconfocal approaches
Biol. Cell (2015) 107, 419–425, DOI: 10.1111/boc.201500004, Short Communication

Martin Weichert, Alexander Lichius, Bert-Ewald Priegnitz, Ulrike Brandt, Johannes Gottschalk, Thorben Nawrath, Ulrike Groenhagen, Nick D. Read, Stefan Schulz, and André Fleißner
Accumulation of specific sterol precursors targets a MAP kinase cascade mediating cell–cell recognition and fusion
PNAS113/42/11877-11882, Published ahead of print, October 5, 2016


Kochan J, Wawro M, and Kasza A (2015). Simultaneous detection of mRNA and protein in single cells using immunofluorescence-combined single-molecule RNA FISH. BioTechniques 59:209-221.
"Huygens deconvolution, Colocalization Analyzer and Surface Renderer were used"

Trcek T, Grosch M, York A, Shroff H, Lionnet T, and Lehmann R
Drosophila germ granules are structured and contain homotypic mRNA clusters
Nature Communications 6 (August 2015) doi 10.1038/ncomms8962]
Huygens deconvolution and pixel shift correction was used here.

Chevy Q, Heubl M, Goutierre M, Backer S, Moutkine I, Eugène E, Bloch-Gallego E, Lévi S. and Poncer JC. KCC2 Gates Activity-Driven AMPA Receptor Traffic through Cofilin Phosphorylation. Journal of Neuroscience 35 (48) 15772-15786
"Huygens deconvolution was used with STED imaging and Cover image.''

Schmitz HP, Jendretzki A, Wittland J, Wiechert J. and Heinisch JJ. Identification of Dck1 and Lmo1 as upstream regulators of the small GTPase Rho5 in Saccharomyces cerevisiae.
Molecular Microbiology (2015) 96(2), 306–324
"Huygens deconvolution was used and the Cover image was provided.''

Unnersjö-Jess D., Scott L., Blom H., Brismar H.
Super-resolution stimulated emission depletion imaging of slit diaphragm proteins in optically
cleared kidney tissue.
Kidney International Kidney International, online publication ahead of print, 7 October 2015
"Huygens deconvolution was used with STED imaging and Cover image.''

Castilho A., Ambrosio A.F., Hartveit E., Veruki M.L.
Disruption of a Neural Microcircuit in the Rod Pathway of the Mammalian Retina by Duabetes Mellitus.
The Journal of Neuroscience 35(13): 5422-5433
"Huygens deconvolution was used with STED imaging and Cover image.''

Brennan F.H., Gordon R, Lao H.W., Biggins P.J., Taylor S.M., Franklin R.J.M., Woodruff T.M., Ruitenberg, M.J.
The Complement Receptor C5aR Controls Acute Inflammation and Astrogliosis following Spinal Cord Injury.
The Journal of Neuroscience 35(16): 6517-6531
Huygens deconvolution was used to create the cover of this J. Neuroscience issue.

Takuya Azuma and Takayuki Kei
Super-resolution spinning-disk confocal microscopy using optical photon reassignment.
Optics Express. 23(11): 15003.

Besseling, T.H., Jose, J. and Blaaderen, A. V.
Methods to calibrate and scale axial distances in confocal microscopy as a function of refractive index.
Journal of Microscopy, 257: 142–150
This paper describes two methods to calibrate Z sampling of 3D confocal microscopes. See also CalibrateAxialDistances.

Zheng P, Bertolet G, Chen Y, Huang S, Liu D
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer.
J Vis. Exp. issue 96

Piwowarczyk K, Wybieralska E, Baran J, Borowczyk J, Rybak P, Kosińska M, Włodarczyk AJ, Michalik M, Siedlar M, Madeja Z, Dobrucki J, Reiss K, Czyż J
Fenofibrate enhances barrier function of endothelial continuum within the metastatic niche of prostate cancer cells
Expert Opin. Ther. Targets (2015) 19(2):163-176

Ponti A, Sevilla Sanchez D, Belyaev Y
Deconvolution of Very Large Data Sets
Imaging & Microscopy (2015) 12:22-24
This article shows that deconvolution of large datasets like SPIM data, can be easily performed with the Huygens Remote Manager/Core.

Dudok B, Barna L, Ledri M, et al.
Cell-specific STORM super-resolution imaging reveals nanoscale organization of cannabinoid signaling
Nature Neuroscience (2015) 18-1:75-86

Zuwała K, Golda A, Kabala W, Burmistrz M, Zdzalik M, Nowak P, Kedracka-Krok S, Zarebski M, Dobrucki J, Florek D, Zeglen S, Wojarski J, Potempa J, Dubin G, Pyrc K.
The nucleocapsid protein of human coronavirus NL63.
https://doi.org/10.1371/journal.pone.0117833|Plos one, 20 Feb 2015]
Huygens was used for deconvolving confocal images.

H. Peuschel, T. Ruckelshausen, C. Cavelius, A. Kraegeloh.
Quantification of internalized silica nanoparticles via STED microscopy.
BioMed Research International, February 2015
Huygens CMLE was used for confocal and STED deconvolution. Huygens Advanced Object Analyzer was applied to segment the cell boundary and to measure the number and geometry of internalized nanoparticles. The authors took the effort to describe the analysis workflow thoroughly.


Chen J, Zhang Z, Li L, Chen BC, Revyakin A, Hajj B, Legant W, Dahan M, Lionnet T, Betzig E, Tjian R, Liu Z (2014), Single-molecule dynamics of enhanceosome assembly in embryonic stem cells
Cell. 2014 Mar 13;156(6):1274-85. doi: 10.1016/j.cell.2014.01.062.

Schoonover, CE, Tapia, J-C, Schilling VC, Wimmer V, Blazeski R, Zhang W, Mason, CA, Bruno RM (2014), Comparative Strength and Dendritic Organization of Thalamocortical and Corticocortical Synapses onto Excitatory Layer 4 Neurons.
Journal of Neuroscience 14 May 2014, 34 (20) 6746-6758.
With Huygens deconvolution an "adequate x and y resolution was achieved to distinguish spine heads and spine necks".

Koch N, Kobler O, Thomas U, Qualmann B, Kessels M.M. (2014), Terminal Axonal Arborization and Synaptic BoutonFormation Critically Rely on Abp1 and the Arp2/3Complex.
PLOS One 9(5): 1-15.

Ting CY, McQueen PG, Pandya N, Lin TY, Yang M, Reddy OV, O'Connor MB, McAuliffe M, Lee CH.
Photoreceptor-derived activin promotes dendritic termination and restricts the receptive fields of first-order interneurons in Drosophila.
Neuron. 2014 Feb 19;81(4):830-846.

Brüggen, B., Meyer, A., Boven, F., Weiler, R. and Dedek, K. (2014), Type 2 wide-field amacrine cells in TH::GFP mice show a homogenous synapse distribution and contact small ganglion cells.
European Journal of Neuroscience. doi: 10.1111/ejn.12813

Ting CY, McQueen PG, Pandya N, Lin TY, Yang M, Reddy OV, O'Connor MB, McAuliffe M, Lee CH.
Photoreceptor-derived activin promotes dendritic termination and restricts the receptive fields of first-order interneurons in Drosophila.
Neuron. 2014 Feb 19;81(4):830-846.

Areti Pantazopouloua, Mario Pinara, Xin Xiang, and Miguel A. Peñalva (2014)
Maturation of late Golgi cisternae into RabE(RAB11) exocytic post-Golgi carriers visualized in vivo.
Molecular Biology of the Cell. August 15, 2014 vol. 25 no. 16 2428-2443

Virginie Buggia-Prévot, Celia G Fernandez, Sean Riordan, Kulandaivelu S Vetrivel, Jelita Roseman, Jack Waters, Vytautas P Bindokas, Robert Vassar and Gopal Thinakaran
Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase.
Molecular Neurodegeneration 2014, 9:1 doi:10.1186/1750-1326-9-1

Schiefermeier N, Scheffler JM, de Araujo ME, Stasyk T, Yordanov T, Ebner HL, Offterdinger M, Munck S, Hess MW, Wickström SA, Lange A, Wunderlich W, Fässler R, Teis D, Huber LA
The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.
J Cell Biol. 2014 May 26;205(4):525-40

Thauerer B, Voegele P, Hermann-Kleiter N, Thuille N, de Araujo ME, Offterdinger M, Baier G, Huber LA, Baier-Bitterlich G.
LAMTOR2-mediated modulation of NGF/MAPK activation kinetics during differentiation of PC12 cells.
PLoS One. 2014 Apr 21;9(4):e95863

Wijetunge LS, Angibaud J, Frick A, Kind PC, Nägerl UV
Stimulated Emission Depletion (STED) Microscopy Reveals Nanoscale Defects in the Developmental Trajectory of Dendritic Spine Morphogenesis in a Mouse Model of Fragile X Syndrome.
J Neurosci, 5302-5313 (2014)

Freeman K, Tao W, Sun H, Soonpaa MH, Rubart M
In situ three-dimensional reconstruction of mouse heart sympathetic innervation by two-photon excitation fluorescence imaging.
J Neuroscience methods 2014, Volume 221: 48–61
Huygens deconvolution and Huygens Colocalization Analyzer was used for this study

Saka SK, Vogts An, Kröhnert K, Hillion F, Rizzoli, SO, Wessels JT (2014)
Correlated optical and isotopic nanoscopy.
Nat Commun. 2014 Apr 10;5:3664

Zhang WI, Röhse H, Rizzoli SO, Opazo, F (2014)
Fluorescent in situ hybridization of synaptic proteins imaged with super-resolution STED microscopy.
published ahead of print April 10, 2014, Microsc. Res. Tech.. doi: 10.1002/jemt.22367

Mace EM, Orange JS (2014)
Lytic immune synapse function requires filamentous actin deconstruction by Coronin 1A. PNAS 2014;
published ahead of print April 23, 2014, doi:10.1073/pnas.1314975111

Mace, EM, Orange, JS
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy.
J. Vis. Exp. (85), e51100, doi:10.3791/51100 (2014).

Kuijk A, Imhof A, Verkuijlen MHW, Besseling TH, van Eck ERH, van Blaaderen A (2014)
Colloidal Silica Rods: Material Properties and Fluorescent Labeling. Part. Part. Syst. Charact.. doi: 10.1002/ppsc.201300329

Padman BS, Bach M, Ramm G (2014)
An Improved Procedure for Subcellular Spatial Alignment during Live-Cell CLEM.
PLoS ONE 9(4): e95967. 22 April 2014 doi: 10.1371/journal.pone.0095967

Bhuvanendran, S.; Salka, K.; Rainey, K.; Sreetama, S.C.; Williams, E.; Leeker, M.; Prasad, V.; Boyd, J.; Patterson, G.H.; Jaiswal, J.K.; Colberg-Poley, A.M.
Superresolution Imaging of Human Cytomegalovirus vMIA Localization in Sub-Mitochondrial Compartments.
Viruses 2014, 6, 1612-1636.

Poenick S1, Jahnke HG, Eichler M, Frost S, Lilie H, Robitzki AA.
Comparative label-free monitoring of immunotoxin efficacy in 2D and 3D mamma carcinoma in vitro models by impedance spectroscopy.
Biosens Bioelectron. 2014 Mar 15;53:370-6. doi: 10.1016/j.bios.2013.09.048. Epub 2013 Sep 29.

Lakdawala SS, Wu Y, Wawrzusin P, Kabat J, Broadbent AJ, Lamirande EW, Fodor E, Altan-Bonnet N, Shroff H, Subbarao K.
Influenza a virus assembly intermediates fuse in the cytoplasm.
Plos Pathogens, 06 Mar 2014

Campinho, M. A., Saraiva, J., Florindo, C., Power, D. M., 2014. Maternal thyroid hormones are essential for neural development in zebrafish. Mol Endocrinol. 28, 1136-1144.
(7):1136-49. 2014 Jul;28

Milewska A, Zarebski M, Nowak P, Stozek K, Potempa J, Pyrc K.
Human coronavirus NL63 utilizes heparan sulfate proteoglycans for attachment to target cells.
Journal of Virology, 03 Sep 2014


Abrahamsson S, Chen J, Hajj B, Stallinga S, Katsov AY, Wisniewski J, Mizuguchi G, Soule P, Mueller F, Dugast Darzacq C, Darzacq X, Wu C, Bargmann CI, Agard DA, Dahan M, Gustafsson MG.
Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.
Nat Methods. 2013 Jan;10(1):60-3. doi: 10.1038/nmeth.2277.

Symes A.J, Eilertsen M, Millar M, Nariculam J, Freeman A, Notara M, Feneley, Patel H.R.H, Masters J.R.W, Ahmed A.
Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue
PLoS One 2013 Dec 27

Westhoff J.H, Giselbrecht S, Schmidts M, Schindler S, Beales P.L, Tönshoff B, Liebel U, Gehrig J.
Development of an Automated Imaging Pipeline for the Analysis of the Zebrafish Larval Kidney
PLoS One 2013 Dec 4;8(12)
This paper nicely shows that Huygens fits perfectly well in high content screening workflows and that Huygens deconvolution benefits image quality and further analysis

Kreutmayer S, Csordas A, Kern J, Maass V, Almanzar G, Offterdinger M, Öllinger R, Maass M, Wick G.
Chlamydia pneumoniae infection acts as an endothelial stressor with the potential to initiate the earliest heat shock protein.
Cell Stress Chaperones. 2013 May;18(3):259-68

Coelho PA, Bury L, Sharif B, Riparbelli MG, Fu J, Callaini G, Gloveremail DM, Zernicka-Goetzemail M.
Spindle Formation in the Mouse Embryo Requires Plk4 in the Absence of Centrioles
Developmental Cell 27:586–597, December 9, 2013
Cover image of the issue was also deconvolved with Huygens Professional

Castañeda V, Cerda M, Santibañez F, Jara J, Pulgar E, Palma K, Lemus CG, Concha M, Hartel S.
Computational Methods for Analysis of Dynamic Events In Cell Migration
Current Molecular Medicine, 2013 - accepted

Wang XD, Su YA, Wagner KV, Avrabos C, Scharf SH, Hartmann J, Wolf M, Liebl C, Kühne C, Wurst W, Holsboer F, Eder M, Deussing JM, Müller MB, Schmidt MV.
Nectin-3 links CRHR1 signaling to stress-induced memory deficits and spine loss.
Nat Neurosci. 2013 Jun;16(6):706-13. Epub 2013 May 5.

Anje Sporbert, Zoltan Cseresnyes, Meike Heidbreder, Petra Domaing, Stefan Hauser, Barbara Kaltschmidt, Christian Kaltschmidt, Mike Heilemann, Darius Widera.
Simple Method for Sub-Diffraction Resolution Imaging of Cellular Structures on Standard Confocal Microscopes by Three-Photon Absorption of Quantum Dots.
PLoS One, 2013 May 21;8(5):e64023

Alireza Ghamari1, Mariëtte P.C. van de Corput, Supat Thongjuea, Wiggert A. van Cappellen, Wilfred van IJcken,Jeffrey van Haren,Eric Soler,Dirk Eick,Boris Lenhard and Frank G. Grosveld.
In vivo live imaging of RNA polymerase II transcription factories in primary cells.
Genes&Development, Accepted March 18, 2013

Lukinavicius G., Umezawa K., Olivier N., Honigmann A., Yang G., Plass T., Mueller V., Reymond L., Corrêa Jr. I.R., Luo Z-G., Schultz G., Lemke E.A., Heppenstall P., Eggeling C., Manley S., Johnsson K.
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.
Nature Chemistry 5, 132–139 (2013)

Lukinavicius G., Lavogina D., Orpinell M., Umezawa K., Reymond L., Garin N., Gönczy P., Johnsson K.
Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex.
Current Biology, 17 January 2013

Redondo N, García-Moreno M, Sanz MA, Carrasco L. (2013). Translation of viral mRNAs that do not require eIF4E is blocked by the inhibitor 4EGI-1.
Virology, 17 Jul 2013, 444(1-2):171-180
"Huygens was used for deconvolving Zeiss LSM510 images"

Kim IH, Racz B, Wang H, Burianek L, Weinberg R, Yasuda R, Wetsel WC and Soderling SH. (2013). Disruption of Arp2/3 Results in Asymmetric Structural Plasticity of Dendritic Spines and Progressive Synaptic and Behavioral Abnormalities.
J. Neuroscience 33 (14) 6081-6092
"Huygens was used for deconvolving the images"


Trcek T, Chao J.A., Larson D.R.,Park H.Y., Zenklusen D., Shenoy S.M., Singer R.H.
Single-mRNA counting using fluorescent in situ hybridization in budding yeast.
Nature Protocols 7, 408–419 (2012)

Malide D, Metais J-Y, Dunbar C.E.
Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by five fluorescent proteins using confocal and multiphoton microscopy.
Blood Sept. 2012, 440636

Micutkova L, Hermann M, Offterdinger M, Hess MW, Matscheski A, Pircher H, Mück C, Ebner HL, Laich A, Ferrando-May E, Zwerschke W, Huber LA, Jansen-Dürr P.
Analysis of the cellular uptake and nuclear delivery of insulin-like growth factor binding protein-3 in human osteosarcoma cells.
Int J Cancer. 2012 Apr 1;130(7):1544-57

Lalle M, Camerini S, Cecchetti S, Sayadi A, Crescenzi M, Pozio E.
Interaction network of the 14-3-3 protein in the ancient protozoan parasite Giardia duodenalis.
J Proteome Res. 2012 May 4;11(5):2666-2683

Mariëtte P. C. van de Corput, Ernie de Boer, Tobias A. Knoch, Wiggert A. van Cappellen, Adrian Quintanilla,Leanna Ferrand, Frank G. Grosveld.
Super-resolution imaging reveals 3D folding dynamics of the β-globin locus upon gene activation
Journal of Cell Science 108522; Advance Online Publication July 5, 2012

F Spira, NS Mueller, G Beck, P von Olshausen, J Beig, R Wedlich-Söldner.
Patchwork organization of the yeast plasma membrane into numerous coexisting domains.
Nature Cell Biology April 29, 2012 vol. 14(6):640-648

Farideh Beigi et al.
Dynamic denitrosylation via S-nitrosoglutathione reductase regulates cardiovascular function.
PNAS March 13, 2012 vol. 109 no. 11 4314-4319

W. Tao, J. Shi, G.W. Dorn, L. Wei. M Rubart
Spatial variability in T-tubule and electrical remodeling of left ventricular epicardium in mouse hearts with transgenic Gαq overexpression-induced pathological hypertrophy.
J Mol Cell Cardiol. Jun 21, 2012 Epub ahead of print

T. Moutinho-Santos, C. Conde, C.E. Sunkel
POLO ensures chromosome bi-orientation by preventing and correcting erroneous chromosome-spindle attachments.
J. Cell Sci. 2012 Feb 1;125(Pt 3):576-583

É.R. Ferreira, A. Bonfim-Melo, R.A. Mortara, D. Bahia
Trypanosoma cruzi extracellular amastigotes and host cell signaling: more pieces to the puzzle.
Front. Immun., 30 Nov 2012, 3:1-6

J-P. Vilardaga, T.J. Gardella, V.L. Wehbi, T.N. Feinstein
Non-canonical signaling of the PTH receptor.
Trends in Pharmacological Sciences, 18 June 2012
Trends in Pharmacological Sciences August issue. Online 18 June 2012
The cover of this issue shows a Huygens deconvolved and alpha blended three-color live endosome.


N Hégarat, E Smith, G Nayak, S Takeda, PA Eyers, H Hochegger.
Aurora A and Aurora B jointly coordinate chromosome segregation and anaphase microtubule dynamics. Includes cover image that was deconvolved with Huygens.
J Cell Biol. 2011 Dec 26;195(7):1103-13

Lalle M, Rosati MA, Bien J, Hehl AB, Pozio E, Tosini F.
Expression of Cryptosporidium parvum Cpa135/CpCCP1 chimeras in Giardia duodenalis: Organization of the protein domains affects the protein secretion pathway.
Exp Parasitol. 2011 Mar;127(3):680-686

Lalle M, Camerini S, Cecchetti S, Blasetti Fantauzzi C, Crescenzi M, Pozio E.
Giardia duodenalis 14-3-3 protein is polyglycylated by a tubulin tyrosin ligase-like member and deglycylated by two metallocarboxypeptidases.
J Biol Chem. 2011 Feb 11;286(6):4471-4484

Lalle M, Currà C, Ciccarone F, Pace T, Cecchetti S, Fantozzi L, Ay B, Breton CB, Ponzi M.
Dematin, a component of the erythrocyte membrane skeleton, is internalized by the malaria parasite and associates with Plasmodium 14-3-3.
J Biol Chem. 2011 Jan 14;286(2):1227-1236

OA Gorleku, AM Barns, GR Prescott, J Greaves, LH Chamberlain.
Endoplasmic reticulum localization of DHHC palmitoyltransferases mediated by lysine-based sorting signals.
J Biol Chem. 2011 Nov 11;286(45):39573-84

J Greaves, JA Carmichael, LH Chamberlain.
The palmitoyl transferase DHHC2 targets a dynamic membrane cycling pathway: regulation by a C-terminal domain.
Mol Biol Cell. 2011 Jun 1;22(11):1887-95

R Peravali, J Gehrig, S Giselbrecht, D Lütjohann, Y Hadzhiev, F Müller, and U Liebel.
Automated feature detection and imaging for high-resolution screening of zebrafish embryos.
BioTechniques, Vol. 50, No. 5, May 2011, pp. 319–324

S Roizard, C Danelon, G Hassaïne, J Piguet, K Schulze, R Hovius, R Tampé, and H Vogel.
Activation of G-Protein-Coupled Receptors in Cell-Derived Plasma Membranes Supported on Porous Beads.
J Am Chem Soc. 2011 Oct 26;133(42):16868-74

E Smith, N Hégarat, C Vesely, I Roseboom, C Larch, H Streicher, K Straatman, H Flynn, M Skehel, T Hirota, R Kuriyama and H Hochegger.
Differential control of Eg5-dependent centrosome separation by Plk1 and Cdk1.
The EMBO Journal (2011) 30, 2233 - 2245, Published online 26 April, 2011
This paper nicely describes the use of Huygens Deconvolution for images acquired with a DeltaVision microscope

L Mensa, G Crespo, MJ Gastinger, J Kabat, S Pérez-del-Pulgar, R Miquel, SU Emerson, RH Purcell, X Forns.
Hepatitis C virus receptors claudin-1 and occludin after liver transplantation and influence on early viral kinetics.
Hepatology (2011) May;53(5):1436-1445

T Feinstein,V Wehbi, J Ardura, D Wheeler, S Ferrandon, T Gardella and J-P Vilardaga.
Retromer terminates the generation of cAMP by internalized PTH receptors.
Nature Chemical Biology 2011 March 27; (7):278-284

R Alonso, C Mazzeo, M C Rodriguez, M Marsh, A Fraile-Ramos, V Calvo, A Avila-Flores, I Merida and M Izquierdo.
Diacylglycerol kinase α regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes.
Cell Death and Differentiation 2011 Jan 21, advance online publication

Jeffrey Kamykowski, Peter Carlton, Siddharth Sehgal and Brian Storrie.
Quantitative immunofluorescence mapping reveals little functional
coclustering of proteins within platelet α-granules.
Blood 2011 118: 1370-1373.Prepublished online May 26, 2011

Lei Lu NTU Singapore, Mark S. Ladinski and Tomas Kirchhausen.
Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear
pore assembly.
JCB published August 8, 2011

Vladimir P. Baklaushev, Gaukhar M. Yusubalieva, Eugene B. Tsitrin, Olga I. Gurina, Nadezhda Ph. Grinenko2, Ilya V. Victorov2, and Vladimir P. Chekhonin.
Visualization of Connexin 43-positive cells of glioma and the periglioma zone by means of intravenously injected monoclonal antibodies.
Drug Delivery, 2011, 1–7, Early Online

Schlapp G, Scavone P, Zunino P and S Härtel.
Development of 3D Architecture of Uropathogenic Proteus mirabilis Batch Culture Biofilms - A Quantitative Confocal Microscopy Approach.J Microbiol Methods, (2011, 87:2, 234-240 (Abstract

Greaves J and Chamberlain LH. Differential palmitoylation regulates intracellular patterning of SNAP25.Journal of Cell Science 2011 124: 1351-1360


Gonzalez D.R., Treuer A.V., Castellanos J., Dulce R.A., and Hare J.M.
Impaired S-Nitrosylation of the Ryanodine Receptor Caused by Xanthine Oxidase Activity Contributes to Calcium Leak in Heart Failure
Journal of Biological Chemistry September 10, 2010, vol 285, 28938-28945

Lalle M, Bavassano C, Fratini F, Cecchetti S, Boisguerin P, Crescenzi M, Pozio E.
Involvement of 14-3-3 protein post-translational modifications in Giardia duodenalis encystations.
Int J Parasitol. (2010), 40, 201-213

Lienkamp S, Ganner A, Boehlke C, Schmidt T, Arnold SJ, Schäfer T, Romaker D, Schuler J, Hoff S, Powelske C, Eifler A, Krönig C, Bullerkotte A, Nitschke R, Kuehn EW, Kim E, Burkhardt H, Brox T, Ronneberger O, Gloy J, Walz G.
Inversin relays Frizzled-8 signals to promote proximal pronephros development.
Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20388-93

Greaves J, Gorleku OA, Salaun C, Chamberlain LH.
Palmitoylation of the SNAP25 protein family: specificity and regulation by DHHC palmitoyl transferases.
J Biol Chem. 2010 Aug 6;285(32):24629-38

Jane E Leadsham and Campbell W Gourlay
cAMP/PKA signaling balances respiratory activity with mitochondria dependent apoptosis via transcriptional regulation
BMC Cell Biology 2010, 11:92

Q le Duc Q, Q Shi, I Blonk, A Sonnenberg, N Wang, D Leckband, J de Rooij.
Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner.
J Cell Biol. 2010 Jun 28;189(7):1107-15.

Marvin Bentley, Deborah C. Nycz, Ashwini Joglekar, Ismene Fertschai, Roland Malli, Wolfgang F. Graier, and Jesse C. Hay
Vesicular Calcium Regulates Coat Retention, Fusogenicity, and Size of Pre-Golgi Intermediates
Mol. Biol. Cell 10.1091/mbc.E09-10-0914

Armin H. Seidl, Edwin W Rubel, and David M. Harris. Virginia Merrill Bloedel Hearing Research Center, Department of Otolaryngology–Head and Neck Surgery, University of Washington, Seattle, Washington.
J. Neurosci. 2010, Jan 6;30(1):70-80.

Ramirez O, García A, Rojas R, Couve A & S Härtel
Confined Displacement Algorithm Determines True and Random Colocalization in Fluorescence Microscopy.
Journal of Microscopy (Sep 1, 2010) 239:3, 173-83 (Abstract)


Nina Jährling, Klaus Becker and hans-Ulrich Dodt. Published December 2009, 3D-reconstruction of blood vessels by
ultramicroscopy, Organogenesis 5:4, 227-230 https://www.landesbioscience.com/journals/organogenesis/06-JahrlingORG5-4.pdf

Zoltan Cseresnyes, Ulf Schwarz and Catherine M Green.
Analysis of replication factories in human cells by super-resolution light microscopy
BMC Cell Biology 2009 10: 88
Huygens Object Analyzer is used in this paper to segment and count objects

Fleissner A, Leeder AC, Roca MG, Read ND, Glass NL. Proc Natl Acad Sci U S A. 2009 Nov 2. (E-pub. ahead of print)
PMID: 19884508 (Pub Med - as supplied by publisher)
Oscillatory recruitment of signaling proteins to cell tips promotes coordinated behavior during cell fusion.

Gernot Stocker, Maria Fischer, Dietmar Rieder, Gabriela Bindea, Simon Kainz, Michael Oberstolz, James G McNally and Zlatko Trajanoski
iLAP: a workflow-driven software for experimental protocol development, data acquisition and analysis
BMC Bioinformatics 2009, 10:390

Christoph Hudemann, Maria Elisabet Lönn, José Rodrigo Godoy, Farnaz Zahedi Avval, Francisco Capani, Arne Holmgren, Christopher Horst Lillig
Identification, Expression Pattern, and Characterization of Mouse Glutaredoxin 2 Isoforms
Antioxidants & Redox Signaling. January 2009, 11(1): 1-14.
Link to cover publisher website

Jose A. Guerrero, Mark Kyei, Susan Russell, Junling Liu, T. Kent Gartner, Brian Storrie and Jerry Ware
Visualizing the von Willebrand factor/glycoprotein Ib-IX axis with a
platelet-type von Willebrand disease mutation
(Blood. Prepublished online Oct 6, 2009; doi:10.1182/blood-)

Seidl AH, Rubel EW.
A simple method for multiday imaging of slice cultures.
Microscopy Research and Technique. 2009 Jun 29.

Patricia A. Soucy, Lewis H. Romer
Endothelial cell adhesion, signaling, and morphogenesis in fibroblast-derived matrix
Matrix Biology Matrix Biology. 273–283. See line item 4 at abstract

Landeira D, Bart JM, Van Tyne D, Navarro M.
Cohesin regulates VSG monoallelic expression in trypanosomes.
J Cell Biol. 2009 Jul 27;186(2):243-54.

M.Oberlaender, P.J. Broser, B. Sakmann & S.Hippler
Shack-Hartmann wave front measurements in cortical tissue for deconvolution of large three-dimensional mosaic transmitted light BRIGHTFIELD micrographs.
Journal of Microscopy Vol. 233, Pt 2, 2009 pp 275-289 abstract

Lei Lu NTU Singapore, Mark S. Ladinsky and Tom Kirchhausen.
Cisternal Organization of the Endoplasmic Reticulum during Mitosis.
Molecular Biology of the Cell, Vol. 20, 3471–3480, August 1, 2009.

Fidorra M, Garcia A, Ipsen J, Härtel S & L Bagatolli.
Lipid domains in giant unilamellar vesicles and their correspondence with equilibrium thermodynamic phases: A quantitative fluorescence microscopy imaging approach.
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1788(10):2142-2149 (2009).


Albanese A, Arosio D, Terreni M, Cereseto A (2008) HIV-1 Pre-Integration Complexes Selectively Target Decondensed Chromatin in the Nuclear Periphery. PLoS ONE 3(6): e2413. doi:10.1371/journal.pone.0002413

Swift F, Birkeland JA, Tovsrud N, Enger UH, Aronsen JM, Louch WE, Sjaastad I, Sejersted OM.
Altered Na+/Ca2+-exchanger activity due to downregulation of Na+/K+-ATPase alpha2-isoform in heart failure.
Cardiovasc Res. 2008 Apr 1;78(1):71-8. Epub 2008 Jan 17. Erratum in: Cardiovasc Res. 2008 Jul 15;79(2):352.

Eiko K. de Jong, Jonathan Vinet, Vesna S. Stanulovic, Michel Meijer, Evelyn Wesseling, Klaas Sjollema, Hendrikus W. G. M. Boddekeand Knut Biber
Expression, transport, and axonal sorting of neuronal CCL21 in large dense-core vesicles.
The FASEB Journal. 2008;22:

Nagotu S, Krikken AM, Otzen M, Kiel JA, Veenhuis M, van der Klei IJ
Peroxisome fission in Hansenula polymorpha requires Mdv1 and Fis1, two proteins also involved in mitochondrial fission.
Traffic Sept 2008, (9):

Y. Belyaev, A. Seitz, S. Terjung, R. Pepperkok
Deconvolution allows reliable imaging of living cells at low fluorescence level.
Poster presented at the ELMI 2008 meeting.
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Sabrina Sonda,* Sasa Stefanic, and Adrian B. Hehl
A Sphingolipid Inhibitor Induces a Cytokinesis Arrest and Blocks Stage Differentiation in Giardia lamblia{triangledown}
Antimicrobial Agents and Chemotherapy, February 2008, p. 563-569, Vol. 52, No. 2 on-line

Sylvain Hugel, Mathias Abegg, Vincenzo de Paola, Pico Caroni, Beat H. Gähwiler and R. Anne McKinney
Dendritic Spine Morphology Determines Membrane-Associated Protein Exchange between Dendritic Shafts and Spine Heads
Cerebral Cortex, doi:10.1093/cercor/bhn118

S. Kumar, G. Ho, K. M. Woo, and L. Zhuo
Achieving cellular resolution for in vivo retinal images of transgenic GFAP-GFP mice via image processing
Optics Express, Vol. 16, Issue 11, pp.

Alberto Sanchez-Diaz, Vanessa Marchesi, Stephen Murray, Richard Jones, Gislene Pereira1, Ricky Edmondson, Terry Allen & Karim Labib.
Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast.
Nature Cell Biology 10, 395 - )
Published online: 16 March 2008|doi:10.1038/ncb1701

Wüstner, D. 2008. Free-cholesterol loading does not trigger phase separation of the fluorescent sterol dehydroergosterol in the plasma membrane of macrophages. Chem. Phys. Lipids. 154, 129-136.

Wüstner, D. and Færgeman, N.J. 2008 Chromatic aberration correction and deconvolution for UV sensitive imaging of fluorescent sterols in cytoplasmic lipid droplets. Cytometry A. 73, 727-744.

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D. Wüstner
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MBC Vol. 18, Issue 1, 211-228, January 2007 get pdf

Gendron ME, Thorin-Trescases N, Villeneuve L, Thorin E.
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Siddhart Sehgal, Brian Storrie
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Dupuis J, Préfontaine A, Villeneuve L, Ruel N, Lefebvre F, Calderone A.
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Seiichiro Nagai, Tadashi Mabuchi, Shuri Hirata, Tomoko Shoda,Tsuyoshi Kasai, Sadaki Yokota, Hiroshi Shitara, Hiromichi Yonekawa, Kazuhiko Hoshi.
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Ann Icking, Monia Amaddii, Mika Ruonala, Stefan Höning, Ritva Tikkanen
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Noemi N. Taniwaki, Fabiana S. Machado, Andre R. Massensini, Renato A. Mortara
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Ilaria Dal Pra, Anna Chiarini, Edward F. Nemeth, Ubaldo Armato.
Roles of Ca(2+)and the Ca(2+)- Sensing Receptor (CARS) in the Expression of Inducible NOS ( Nitric Oxide Synthase)-2 and Its BH4 (Tetrahydrobiopterin)-Dependent Activation in Cytokine-Stimulated Adult Human Astrocytes.
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Anna Chianini, Ilaria Dalpra, Lia menapace, Raffaella Pacchiana, James F. Whitfield and Ubaldo Armato.
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Sung Wu Rhee, Tregei Starr, Kimberly Forsten-Williams and Brian Storrie
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R. R. Duncan, A. Bergmann, M. A. Cousin, D. K. Apps & M. J. Shipston
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Engstler M, Thilo L, Weise F, Grünfelder CG, Schwarz H, Boshart M and Overath P
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Frieden M, James D, Castelbou C, Danckaert A, Martinou JC and Demaurex N
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Grailhe R, de Carvalho LP, Paas Y, Le Poupon C, Soudant M, Bregestovski P, Changeux JP and Corringer, PJ.
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Martinez-Campos M, Basto R, Baker J, Kernan M and Raff JW
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Planat-Bénard V, Menard C, André M, Puceat M, Perez A, Garcia-Verdugo J-M, Pénicaud L and Casteilla, L.
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Focusing light on infection in four dimensions
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Schutte B, Henfling M, Kölgen W, Bouman M, Meex S, Leers MPG, Nap M, Björklund V, Björklund P, Björklund B, Lane EB, Omary MB, Jörnvall H and Ramaekers FCS
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Ausma J, van der Velden HMW, Lenders M-H, van Ankeren EP, Jongsma HJ, Ramaekers FCS, Borgers M and Allessie MA
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Balthasar N, Mery P-F, Magoulas CB, Mathers KE, Martin A, Mollard P and Robinson ICAF
Growth Hormone-Releasing Hormone (GHRH) Neurons in GHRH-Enhanced Green Fluorescent Protein Transgenic Mice: A Ventral Hypothalamic Network
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Bantignies F, Grimaud C, Lavrov S, Gabut M, Cavalli G.
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Bürgi S, Baltensperger K and Honegger UE
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Daly CJ and McGrath JC
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Demaurex N and Frieden M
Measurements of the free luminal ER Ca2+ concentration with targeted cameleon fluorescent proteins
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De Simoni A, Griesinger CB and Edwards FA
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Evans JG, Correia I, Krasavina O, Watson N and Matsudaira P
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Fusco D, Accornero N, Lavoie B, Shenoy SM, Blanchard J-M, Singer RH and Bertrand E
Single mRNA Molecules Demonstrate Probabilistic Movement in Living Mammalian Cells
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Galvan V, Logvinova A, Sperandio S, Ichijo H and Bredesen DE
Type 1 Insulin-like Growth Factor Receptor (IGF-IR) Signaling Inhibits Apoptosis Signal-regulating Kinase 1 (ASK1)
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Gerzanich V, Ivanov A, Ivanova S, Yang JB, Zhou H, Dong Y and Simard JM
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Gerzanich V, Ivanova S, van der Heiden M, Zhou H and Simard JM
Trans-Cellular Proliferating Cell Nuclear Antigen Gene Activation in Cerebral Vascular Smooth Muscle by Endothelial Oxidative Injury In Vivo
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Gimona M, Kaverina I, Resch GP, Vignal E and Burgstaller G
Calponin Repeats Regulate Actin Filament Stability and Formation of Podosomes in Smooth Muscle Cells
Molecular Biology of the Cell (2003) Vol 14, pp

Goshima G and Vale RD
The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line
The Journal of Cell Biology (2003) Volume 162, Number 6 pp

Grünfelder CG, Engstler M, Weise F, Schwarz H, Stierhof Y-D, Morgan GW, Field MC and Overath P
Endocytosis of a Glycosylphosphatidylinositol-anchored Protein via Clathrin-coated Vesicles, Sorting by Default in Endosomes, and Exocytosis via RAB11-positive Carriers
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Hauf S, Cole RW, LaTerra S, Zimmer C, Schnapp G, Walter R, Heckel A, van Meel J, Rieder CL, and Peters J-M
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Heilbronn R, Engstler M, Weger S, Krahn A, Schetter C and Boshart M
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Homma N, Takei Y, Tanaka Y, Nakata T, Terada S, Kikkawa M, Noda Y and Hirokawa N
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Houzet L, Battini JL, Bernard E, Thibert V, Mougel M.
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Klauke N, Smith GL and Cooper J
Stimulation of Single Isolated Adult Ventricular Myocytes within a Low Volume Using a Planar Microelectrode Array
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Lakemond CMM, de Jongh HHJ, Paques M, van Vliet T, Gruppen H, Alphons and Voragen GJ
Gelation of soy glycinin; influence of pH and ionic strength on network structure in relation to protein conformation
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Lalevee N, Resin V, Arnaudeau S, Demaurex N, Rossier MF
Intracellular Transport of Calcium from Plasma Membrane to Mitochondria in Adrenal H295R Cells: Implication for Steroidogenesis
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Lorger M, Engstler M, Homann M and Göringer HU
Targeting the Variable Surface of African Trypanosomes with Variant Surface Glycoprotein-Specific, Serum-Stable RNA Aptamers
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Martin-Verdeaux S, Pombo I, Iannascoli B, Roa M, Varin-Blank N, Rivera J and Blank U
Evidence of a role for Munc18-2 and microtubules inmast cell granule exocytosis
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Malli R, Frieden F, Osibow K, Zoratti C, Mayer M, Demaurex N and Graier WF
Sustained Ca2+ Transfer across Mitochondria Is Essential for Mitochondrial Ca2+ Buffering, Store-operated Ca2+ Entry, and Ca2+ Store Refilling
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Metzen E, Berchner-Pfannschmidt U, Stengel P, Marxsen JH, Stolze I, Klinger M, Huang WQ, Wotzlaw C, Hellwig-Bürgel T, Jelkmann W, Acker H and Fandrey J
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Mußmann R, Janssen H, Calafat J, Engstler M, Ansorge I, Clayton C and Borst P
The expression level determines the surface distribution of the transferrin receptor in Trypanosoma brucei
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Nair P, Schaub BE and Rohrer J
Characterization of the Endosomal Sorting Signal of the Cation-Dependent Mannose 6-Phosphate Receptor
J.Biol.Chem. (2003) Vol 278, No. 27 pp

Oleynikov Y and Singer RH
Real-Time Visualization of ZBP1 Association with beta-actin mRNA during Transcription and Localization
Current Biology (2003) Vol. 13 pp 199-207

Perez-Terzic C, Behfar A, Mery A, van Deursen JMA, Terzic A, Pucéat M
Structural Adaptation of the Nuclear Pore Complex in Stem Cell-Derived Cardiomyocytes
Circ Res (2003) 92 pp 444-452

Planat-Bénard V, Menard C, André M, Pucéat M, Perez A, Garcia-Verdugo J-M, Pénicaud L and Casteilla L
Spontaneous Cardiomyocyte Differentiation From Adipose Tissue Stroma Cells
Circ. Res. (2003); 10.1161/01.RES..43271.47

Pucéat M, Travo P, Quinn MT and Fort P
A Dual Role of the GTPase Rac in Cardiac Differentiation of Stem Cells
Molecular Biology of the Cell (2003) Vol. 14, pp

Regnard C, Fesquet D, Janke C, Boucher D, Desbruyeres E, Koulakoff A, Insina C, Travo P, Edde B
Characterisation of PGs1, a subunit of a protein complex co-purifying with tubulin polyglutamylase
J Cell Sci. (2003);116(Pt 20), pp

Rogers SL, Wiedemann U, Stuurman N and Vale RD
Molecular requirements for actin-based lamella formation in Drosophila S2 cells
The Journal of Cell Biology (2003) Volume 162, Number 6 pp

Stepanova T, Slemmer J, Hoogenraad CC, Lansbergen G, Dortland B, De Zeeuw CI, Grosveld F, van Cappellen G, Akhmanova A and Galjart N
Visualization of Microtubule Growth in Cultured Neurons via the Use of EB3-GFP (End-Binding Protein 3-Green Fluorescent Protein)
The Journal of Neuroscience (2003) 23(7) pp

Thorn H, Stenkula KG, Karlsson M, Örtegren U, Nystrom FH, Gustavsson J and Strålfors P
Cell surface orifices of caveolae and localization of caveolin to the necks of caveolae in adipocytes
Mol. Biol. Cell (2003) 14 pp

Verschure PJ, van der Kraan I, Manders EMM, Hoogstraten D, Houtsmuller AB and van Driel R
Condensed chromatin domains in the mammalian nucleus are accessible to large macromolecules
EMBO reports (2003) 4, pp 861-866

Voet T, Liebe B, Labaere C, Marynen P and Scherthan H
Telomere-independent homologue pairing and checkpoint escape of accessory ring chromosomes in male mouse meiosis
The Journal of Cell Biology (2003) Vol. 162, Number 5, pp 795-807

Wouterlood FG , Böckers T and Witter MP
Synaptic contacts between identified neurons visualized in the confocal laserscanning microscope. Neuroanatomical tracing combined with immunofluorescence detection of postsynaptic density proteins and target neuron-markers
J. Neuroscience Methods (2003) 128 pp 129-142


Arnaudeau S, Frieden M, Nakamura K, Castelbou C, Michalak M and Demaurex N
Calreticulin differentially modulates calcium uptake and release in the endoplasmic reticulum and mitochondria
J. Biol.Chem. (2002) 277 pp

Behfar A, Zingman LV, Hodgson DM, Rauzier J-M, Kane GC, Terzic A and Pucéat M
Stem cell differentiation requires a paracrine pathway in the heart
The FASEB Journal (2002) 16 pp

Brockhaus J and Deitmer JW
Long-lasting modulation of synaptic input to Purkinje neurons by Bergmann glia stimulation in rat brain slices
Journal of Physiology (2002) 545.2 pp 581-593

Charrasse S, Meriane M, Comunale F, Blangy A and Gauthier-Rouvière C
N-cadherin-dependent cell-cell contact regulates Rho GTPases and ß-catenin localization in mouse C2C12 myoblasts
The Journal of Cell Biology (2002) Volume 158, Number 5, pp 953-965

Claycomb JM, MacAlpine DM, Evans JG, Bell SP and Orr-Weaver TL
Visualization of replication initiation and elongation in Drosophila
The Journal of Cell Biology (2002), Volume 159, Number 2, pp 225-236

Conte N, Charafe-Jauffret E, Delaval B, Adélaïde J, Ginestier C, Geneix J, Isnardon D, Jacquemier J and Birnbaum D
Carcinogenesis and translational controls: TACC1 is down-regulated in human cancers and associates with mRNA regulators
Oncogene (2002) 21, pp 5619 - 5630

De Renzis S, Sönnichsen B and Zerial M
Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes
Nature Cell Biology (2002) 4, pp 124-133

Dzwinel W, Yuen DA and Boryczko K
Mesoscopic dynamics of colloids simulated with dissipative particle dynamics and fluid particle model
J Mol Model (2002) 8, pp 33-43

Edinger RS, Watkins SC, Pearce D and Johnson JP
Effect of immunosuppressive agents on glucocorticoid receptor function in A6 cells
Am J Physiol Renal Physiol (2002) 283, pp F254-F261

Engel U, Oezbek S, Engel R, Petri B, Lottspeich F and Holstein TW
Nowa, a novel protein with minicollagen Cys-rich domains, is involved in nematocyst formation in Hydra
Journal of Cell Science (2002) 115, pp

Estrach S, Schmidt S, Diriong S, Penna A, Blangy A, Fort P and Debant A
The Human Rho-GEF Trio and Its Target GTPase RhoG Are Involved in the NGF Pathway, Leading to Neurite Outgrowth
Current Biology (2002), Vol. 12, pp 307-312 get pdf

Furstoss O, Dorey K, Simon V, Barila D, Superti-Furga G and Roche S
c-Abl is an effector of Src for growth factor-induced c-myc expression and DNA synthesis
The EMBO Journal (2002) Vol 21 No 4 pp 514-524

Furuyashiki T, Arakawa Y, Takemoto-Kimura S, Bito H and Narumiya S
Multiple spatiotemporal modes of actin reorganization by NMDA receptors and voltage-gated Ca21 channels
PNAS (2002) vol.99 no. 22 pp

Gräf, R.
DdNek2, the first non-vertebrate homologue of human Nek2, is involved in the formation of microtubuleorganizing centers
Journal of Cell Science (2002) 115, pp
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Grünfelder CG, Engstler M, Weise F, Schwarz H, Stierhof Y-D, Boshart M and Overath P
Accumulation of a GPI-Anchored Protein at the Cell Surface Requires Sorting at Multiple Intracellular Levels
Traffic (2002), 3, pp 547-559

Haj FG, Verveer PJ, Squire A, Neel BG and Bastiaens PIH
Imaging Sites of Receptor Dephosphorylation by PTP1B on the Surface of the Endoplasmic Reticulum
Science (2002) pp

Hannus M, Feiguin F, Heisenberg C-F and Eaton S
Planar cell polarization requires Widerborst, a B' regulatory subunit of protein phosphatase 2A
Development (2002) 129, pp

Kern G, Bösch ST, Unterhuber E and Pelster B
Mechanisms of acid secretion in pseudobranch cells of rainbow trout Oncorhynchus
The Journal of Experimental Biology (2002) 205 pp 2943-2954

Koberna K, Malínský J, Pliss A, Masata M, Vecerová J, Fialová M, Bednár J and Raska I
Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of "Christmas trees" in situ
The Journal of Cell Biology (2002) Volume 157, Number 5, pp 743-748 get pdf

de Krom M,van de Corput M, von Lindern M, Grosveld F and Strouboulis J
Stochastic Patterns in Globin Gene Expression Are Established prior to Transcriptional Activation and Are Clonally Inherited
Molecular Cell (2002) Vol. 9, pp
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Landmann, L.
Deconvolution improves colocalization analysis of multiple fluorochromes in 3D confocal data sets more than filtering techniques
Jo. Micr. (2002) V 208, Pt 2, pp 134-147

Li J, Pucéat M, Perez-Terzic C, Mery A, Nakamura K, Michalak M, Krause K-H and Jaconi ME
Calreticulin reveals a critical Ca2+ checkpoint in cardiac myofibrillogenesis
The Journal of Cell Biology (2002) Volume 158, Number 1, pp 103-113 get pdf

Malínský J, Koberna K, Bednár J, Stulík J and Raska I
Searching for active ribosomal genes in situ: light microscopy in light of the electron beam
Journal of Structural Biology (2002) 140 pp 227-231

Mary S, Charrasse S, Meriane M, Comunale F, Travo P, Blangy A and Gauthier-Rouvière C
Biogenesis of N-Cadherin-dependent Cell-Cell Contacts in Living Fibroblasts Is a Microtubuledependent Kinesin-driven Mechanism
Molecular Biology of the Cell Vol. (2002) 13, pp 285-301 get pdf

Polesello C, Delon I, Valenti P, Ferrer P and Payre F
Dmoesin controls actin-based cell shape and polarity during Drosophila melanogaster oogenesis
Nature Cell Biology (2002) 4, pp 782-789

Rehberg, M. and Gräf, R.
Dictyostelium EB1 Is a Genuine Centrosomal Component Required for Proper Spindle Formation
Molecular Biology of the Cell (2002) Vol. 13, pp
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Rothen-Rutishauser B, Riesen FK, Braun A, Gunthert M, Wunderli-Allenspach H.
Dynamics of tight and adherens junctions under EGTA treatment.
J Membr Biol. (2002) Jul 15;188(2):151-62

Stahl A, Evans JG, Pattel S, Hirsch D and Lodish HF
Insulin Causes Fatty Acid Transport Protein Translocation and Enhanced Fatty Acid Uptake in Adipocytes
Developmental Cell (2002) 2, pp 477-488

Verschure PJ, van der Kraan I, Enserink JM, Moné MJ, Manders EMM and van Driel R
Large-scale Chromatin Organization and the Localization of Proteins Involved in Gene Expression in Human Cells
J.Histochem.Cytochem. (2002) 50, pp

Verveer, PJ.
Comment on 'Generalized approach for accelerated maximum likelihood based image restoration applied to three-dimensional fluorescence microscopy', by L. H. Schaefer, D. Schuster and H. Herz, J. Microsc. 204,
Journal of Microscopy (2002), Vol. 208, Pt 1, pp 1-2

Wouterlood FG, Vinkenoog M and van den Oever M
Tracing tools to resolve neural circuits
Network: Comput. Neural Syst. (2002) 13, pp 327-342

Wouterlood FG, van Haeften T, Blijleven N, Perez-Templado P and Perez-Templado H
Double-Label Confocal Laser-Scanning Microscopy, Image Restoration, and Real-Time Three-Dimensional Reconstruction to Study Axons in the Central Nervous System and Their Contacts With Target Neurons
Applied Immunohistochemistry & Molecular Morphology (2002) 10(1), pp 85-95

Ziese U, Janssen AH, Murk J-L, Geerts WJC, van der Krift T , Verkleij AJ and Koster AJ
Automated high-throughput electron tomography by pre-calibration of image shifts
Journal of Microscopy (2002) Vol 205, Pt2, pp 187-200 get pdf

Noordmans HJ, van der Voort HTM and Smeulders AWM
Spectral Volume Rendering
IEEE Transactions On Visualization And Computer Graphics (2002) VOL. 6, NO. 3 pp 196-207

Landmann, L.
Deconvolution improves colocalization analysis of multiple fluorochromes in 3D confocal data sets more than filtering techniques
Jo. Micr. (2002) V 208, Pt 2, pp 134-147

Landmann, L.
Deconvolution improves colocalization analysis of multiple fluorochromes in 3D confocal data sets more than filtering techniques
Jo. Micr. (2002) V 208, Pt 2, pp 134-147


L, Mittelman L, Tsarfaty I, Shiloh Y and Rotman G
Nuclear Retention of ATM at Sites of DNA Double Strand Breaks
The Journal Of Biological Chemistry (2001) Vol. 276, No. 41, pp

Arnaudeau S, Kelley WL, Walsh JV, Jr. and Demaurex N
Mitochondria Recycle Ca2+ to the Endoplasmic Reticulum and Prevent the Depletion of Neighboring Endoplasmic Reticulum Regions
The Journal Of Biological Chemistry (2001) Vol. 276, No. 31, pp
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Baertschi AJ, Monnier D, Schmidt U, Levitan ES, Fakan S and Roatti A
Acid Prohormone Sequence Determines Size, Shape, and Docking of Secretory Vesicles in Atrial Myocytes
Circ Res.(2001) 89:e23-e29

Bony C, Roche S, Shuichi U, Sasaki T, Crackower MA, Penninger J, Mano H and Pucéat M
A Specific Role of Phosphatidylinositol 3-Kinase g: A Regulation of Autonomic Ca2+ Oscillations in Cardiac Cells
The Journal of Cell Biology (2001) Volume 152, Number 4, pp 717-727 get pdf

Cau J, Faure S, Comps M, Delsert C and Morin N
A novel p21-activated kinase binds the actin and microtubule networks and induces microtubule stabilization
The Journal of Cell Biology (2001) Volume 155, Number 6, pp get pdf

Chen BK, Rousso I, Shim S and Kim PS
Efficient assembly of an HIV-1 MLV Gag-chimeric virus in murine cells
PNAS (2001) vol 98 no26 pp.

Den Blaauwen T, Lindqvist A, Löwe J and Nanninga N
Distribution of the Escherichia coli structural maintenance of chromosomes (SMC)-like protein MukB in the cell
Molecular Microbiology (2001) 42(5), pp

Guild SB, Murray AT, Wilson ML, Wiegand UK, Apps DK, Jin Y, Rindler M, Roder J and Jeromin A
Over-expression of NCS-1 in AtT-20 cells affects ACTH secretion and storage
Molecular and Cellular Endocrinology (2001) 184, pp 51-63

Habermann FA, Cremer M, Walter J, Kreth G, von Hase J, Bauer K, Wienberg J, Cremer C, Cremer T and Solovei I
Arrangements of macro- and microchromosomes in chicken cells
Chromosome Research (2001) 9, pp 569-584 get pdf

Heun P, Laroche T, Raghuraman MK and Gasser SM
The Positioning and Dynamics of Origins of Replication in the Budding Yeast Nucleus
J Cell Biol (2001) 152, pp 385-400

Lengronne A, Pasero P, Bensimon A and Schwob E
Monitoring S phase progression globally and locally using BrdU incorporation in TK+ yeast strains
Nucleic Acids Research (2001) Vol 29, No 7 pp get pdf

Meesen G and Poffijn A
Semi-automated analysis of three-dimensional track images
Radiation Measurements (2001) 34 pp 161-165

Ogris M, Steinlein P, Carotta S, Brunner S and Wagner E
DNA/polyethylenimine transfection particles: Influence of ligands, polymer size, and PEGylation on internalization and gene expression
AAPS PharmSci (2001) 3 (3) article 21
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Perez-Terzic C, Gacy AM, Bortolon R, Dzeja PP, Puceat M, Jaconi M, Prendergast FG, and Terzic A
Directed Inhibition of Nuclear Import in Cellular Hypertrophy

Prem, C. and Pelster, B.
Swimbladder gas gland cells cultured on permeable supports regain their characteristic polarity
The Journal of Experimental Biology (2001) 204, pp

Rietdorf J, Ploubidou A, Reckmann I, Holmström A, Frischknecht F, Zettl M, Zimmermann T and Way M
Kinesin-dependent movement on microtubules precedes actin-based motility of vaccinia virus
Nature Cell Biology (2001) 3, pp 992 – 1000

Rohrer, J. and Kornfeld, R.
Lysosomal Hydrolase Mannose 6-Phosphate Uncovering Enzyme Resides in the trans-Golgi Network
Molecular Biology of the Cell (2001) Vol 12, pp

Rust RC, Landmann L, Gosert R, Luen Tang B, Hong W, Hauri H-P, Egger D and Bienz K
Cellular COPII Proteins Are Involved in Production of the Vesicles That Form the Poliovirus Replication Complex
Journal Of Virology (2001) Vol 75(20), pp

Sander A, Hesser BA and Witzemann V
MuSK induces in vivo acetylcholine receptor clusters in a ligand-independent manner
The Journal of Cell Biology (2001) Volume 155, Number 7, pp

Stanek D, Koberna K, Pliss A, Malínsky J, Masata M, Vecerová J, Risueño M-C and Raska I
Non-isotopic mapping of ribosomal RNA synthesis and processing in the nucleolus
Chromosoma (2001) 110, pp 460-470

Veraverbeke EA, Van Bruaene N, Van Oostveldt P and Nicolaï BM
Non destructive analysis of the wax layer of apple (Malus domestiva Borkh) by means of confocal laser scanning microscopy
Planta (2001) 213, pp 525-533

Verheggen C, Mouaikel J, Thiry M, Blanchard J-M, Tollervey D, Bordonné R, Lafontaine DLJ and Bertrand E
Box C/D small nucleolar RNA trafficking involves small nucleolar RNP proteins, nucleolar factors and a novel nuclear domain
The EMBO Journal (2001) Vol.20 N019 pp

Vietor I, Bader T, Paiha K and Huber LA
Peturbation of the tight junction permeability barrier by occludin loop peptides activates b-catenin/TCF/LEF-mediated transcription
EMBO reports (2001) vol 2, no 4, pp 306-312

Vignal E, Blangy A, Martin M, Gauthier-Rouvière C and Fort P
Kinectin Is a Key Effector of RhoG Microtubule-Dependent Cellular Activity

Voermans C, Anthony EC, Mul E, van der Schoot E and Hordijk P
SDF-1-induced actin polymerization and migration in human hematopoietic progenitor cells
Experimental Hematology pp

Walther TC, Fornerod M, Pickergill H, Goldberg M, Allen TD and Mattaj IW
The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins
The EMBO Journal (2001) Vol.20 pp

Wossink J, Karst H, Mayboroda O and Joëls M
Morphological And Functional Properties Of Rat Dentate Granule Cells After Adrenalectomy
Neuroscience Vol. (2001) 108, No. 2, pp 263-272

Wunderlich W, Fialka I, Teis D, Alpi A, Pfeifer A, Parton RG, Lottspeich F and Huber LA
A Novel 14-Kilodalton Protein Interacts with the Mitogen-activated Protein Kinase Scaffold MP1 on a Late Endosomal/Lysosomal Compartment
The Journal of Cell Biology (2001) Volume 152, Number 4, pp 765-776 get pdf

Bergsma CBJ, Streekstra GJ, Smeulders AWM and Manders EMM
Velocity Estimation of Spots in Three-Dimensional Confocal Image Sequences of Living Cells
Cytometry (2001) 43 pp 261-272
"Huygens Visualitzation tools were used"


Dukuzumuremyi J-M, Rosqvist R, Hallberg B, Åkerström B, Wolf-Watz H and Schesser K
The Yersinia Protein Kinase A Is a Host Factor Inducible RhoA/Rac-binding Virulence Factor
The Journal Of Biological Chemistry (2000) Vol. 275, No. 45, pp

Geissler H, Ullmann R and Soldati T
The Tail Domain of Myosin M Catalyses Nucleotide Exchange on Rac1 GTPases and Can Induce Actin-Driven Surface Protrusions
Traffic (2000) 1 pp 399-41

Frei C and Gasser SM
The yeast Sgs1p helicase acts upstream of Rad53p in the DNA replication checkpoint and colocalizes with Rad53p in S-phase-specific foci
Genes & Development (2000) 14 pp 81-96

Geissler H, Ullmann R and Soldati T
The Tail Domain of Myosin M Catalyses Nucleotide Exchange on Rac1 GTPases and Can Induce Actin-Driven Surface Protrusions
Traffic (2000) 1 pp 399-410

Hetzer M, Bilbao-Cortés D, Walther TC, Gruss OJ and Mattaj IW
GTP Hydrolysis by Ran Is Required for Nuclear Envelope Assembly
Molecular Cell (2000) 5: pp
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Hoek M, Engstler M and Cross GAM
Expression-site-associated gene 8 (ESAG8) of Trypanosoma bruceiis apparently essential and accumulates in the nucleolus
Journal of Cell Science (2000) 113, pp get pdf

Huber LA, Fialka I, Paiha K, Hunziker W, Sacks DB, Bähler M, Way M, Gagescu R and Gruenberg J
Both Calmodulin and the Unconventional Myosin Myr4 Regulate Membrane Trafficking Along the Recycling Pathway of MDCK Cells
Traffic (2000) 1: pp 494-503

Jiao J-H, Baumann P, Baron A, Roatti A, Pence RA and Baertschi AJ
Sulfonylurea receptor ligands modulate stretch-induced ANF secretion in rat atrial myocyte culture
Am J Physiol Heart Circ Physiol (2000) 278 pp H2028-H2038

Lang T, Wacker I, Wunderlich I, Rohrbach A, Giese G, Soldati T and Almers W
Role of Actin Cortex in the Subplasmalemmal Transport of Secretory Granules in PC-12 Cells
Biophysical Journal (2000) Volume 78 pp
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Langeveld SMJ, van Wijk R, Stuurman N, Klijne JW and de Pater S
B-type granule containing protrusions and interconnections between amyloplasts in developing wheat endosperm revealed by transmission electron microscopy and GFP expression
Journal of Experimental Botany (2000) Vol 51, No 349, pp

Loffing J, Pietri L, Aregger F, Bloch-Faure M, Ziegler U, Meneton P, Rossier BC and Kaissling B
Differential subcellular localization of ENaC subunits in mouse kidney in response to high- and low-Na diets
Am J Physiol Renal Physiol (2000) 279: F252-F258

Meyer N, Jaconi M, Landopoulou A , Fort P, Puceat M
A fluorescent reporter gene as a marker for ventricularspecification in ES-derived cardiac cells
FEBS Letters (2000) 478 pp 151-158
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Ogris M, Wagner E and Steinlein P
A versatile assay to study cellular uptake of gene transfer complexes by flow cytometry
Biochimica et Biophysica Acta pp 237-243

Osten P, Khatri L, Perez JL, Köhr G, Giese G, Daly C, Schulz TW, Wensky A, Lee LM and Ziff EB
Mutagenesis Reveals a Role for ABP/GRIP Binding to GluR2 in Synaptic Surface Accumulation of the AMPA Receptor
Neuron (2000) 27, pp 313-325

Prem C, Salvenmoser W, Würtz J and Pelster B
Swim bladder gas gland cells produce surfactant: in vivo and in culture
Am J Physiol Regulatory Integrative Comp Physiol (2000) 279, pp R2336-R2343

Rahner C, Stieger B and Landmann L
Apical endocytosis in rat hepatocytes in situ involves clathrin, traverses a subapical compartment, and leads to lysosomes
Gastroenterology (2000) 119, pp

Rothen-Rutishauser B, Braun A, Günthert M and Wunderli-Allenspach H
Formation of Multilayers in the Caco-2 Cell Culture Model: A Confocal Laser Scanning Microscopy Study
Pharmaceutical Research (2000), Vol. 17, No. 4, pp 460-465

Schwarz EC, Neuhaus EM, Kistler C, Henkel AW and Soldati T
Dictyostelium myosin IK is involved in the maintenance of cortical tension and affects motility and phagocytosis
J Cell Science (2000) 113, pp 621-633

Sönnichsen B, De Renzis S, Nielsen E, Rietdorf J and Zerial M
Distinct Membrane Domains on Endosomes in the Recycling Pathway Visualized by Multicolor Imaging of Rab4, Rab5, and Rab11
J Cell Biology (2000) 149, 4, pp 901-913
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Strohmaier AR, Porwol T, Acker H and Spiess E
Three-dimensional organization of microtubules in tumor cells studied by confocal laser scanning microscopy and computer-assisted deconvolution and image reconstruction
Cells Tissues Organs (2000);167(1) pp 1-8

Stuurman N, Pacios Bras C, Schlaman HRM, Wijfjes AHM, Bloemberg G, and Spaink HP
Use of Green Fluorescent Protein Color Variants Expressed on Stable Broad-Host-Range Vectors to Visualize Rhizobia Interacting with Plants
MPMI (2000) Vol. 13, No. 11 pp
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van der Velden HMW, Ausma J, Rook MB, Hellemons AJCGM, van Veen TAAB, Allessie MA, Jongsma HJ
Gap junctional remodeling in relation to stabilization of atrial fibrillation in the goat
Cardiovascular Research (2000) 46 pp 476-486

Vignal E, De Toledo M, Comunale F, Ladopouloui A, Gauthier-Rouvière C, Blangy A and Fort P
Characterization of TCL, a New GTPase of the Rho Family related to TC10 and Cdc42
The Journal of Biological Chemistry (2000) Vol 275, No 46, pp

Habermann FA (2000)
Untersuchungen zur Anordnung von Makro- und Mikrochromosomen im Zellkern embryonaler Fibroblasten und Neuronen von Gallus domesticus.
Dissertation zum Erwerb des Doktorgrades der Medizin an der Medizinischen Fakultät der Ludwig-Maximilians-Universität zu München.
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Broers JLV, Machiels BM, van Eys GJJM, Kuijpers HJH, Manders EMM, van Driel R and Ramaekers FCS
Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins
J Cell. Science 112, (1999) pp

de Grauw CJ, Vroom JM, van der Voort HTM and Gerritsen HC
Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens
Applied Optics (1999) V 38, No.28, pp

Diaspro A, Corosu M, Ramoino P and Robello M
Adapting a Compact Confocal Microscope System to a Two-Photon Excitation Fluorescence Imaging Architecture
Microscopy Research and Technique (1999) 47, pp 196-205 get pdf

Fialka I, Steinlein P, Ahorn H, Böck G, Burbelo PD, Haberfellner M, Lottspeich F, Paiha K, Pasquali C and Huber LA
Identification of Syntenin as a Protein of the Apical Early Endocytic Compartment in Madin Darby Canine Kidney Cells
The Journal of Biological Chemistry (1999) Vol 274, No 37, pp

Merrifield CJ, Moss SE, Ballestrem C, Imhof BA, Giese G,Wunderlich I and Almers W
Endocytic vesicles move at the tips of actin tails in cultured mast cells
Nature Cell Biology (1999) Vol 1 No 1 pp 72-74
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Oliferenko S, Paiha K, Harder T, Gerke V, Schwärzler C, Schwarz H, Beug H, Günthert U and Huber LA
Analysis of CD44-containing Lipid Rafts: Recruitment of Annexin II and Stabilization by the Actin Cytoskeleton
J Cell Biology (1999) 147, 4, pp 843-854

Porwol T, Strohmaier A-R and Spiess E
Methods in Enzymology (1999) Vol 307 pp 108-118

Verschure PJ, van der Kraan I, Manders EMM and van Driel R
Spatial relationship between transcription sites and chromosome territories
J Cell Biology (1999) 147, 1, pp 13-24

Visser AE and Aten JA
Chromosomes as well as chromosomal subdomains constitute distinct units in interphase nuclei
J Cell Science (1999) 112, pp

Yang S-H, Larsson O, Brändström R, Bertorello AM, Leiberger B, Leiberger IB, Moede T, Köhler M, Meister B and Berggren P-O
Syntaxin 1 interacts with the LD subtype of voltage-gated Ca2+ channels in pancreatic b cells
Proc Natl Acad Sci USA (1999) Vol 96, pp , Cell Biology

de Grauw CJ, Vroom JM, van der Voort HTM and Gerritsen HC
Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens
Applied Optics (1999) V 38, No.28, pp


Andersson H, Baechi T, Hoechl M and Richter C
Autofluorescence of living cells
Journal of microscopy (1998) Vol 191 Pt 1, pp 1-7

Buchenau P, Hodgson J, Strutt H and Arndt-Jovin DJ
The Distribution of Polycomb-Group Proteins During Cell Division and Development in Drosophila Embryos: Impact on Models for Silencing
J of Cell Biology (1998) 141, 2, 4 pp 69-481

Rothen-Rutishauser BM, Messerli MJ, van der Voort H, Guenthert M and Wunderli-Allenspach H
Deconvolution Combined with Digital Colocalization Analysis to Study the Spatial Distribution of Tight and Adherens Junction Proteins
Jo. of Computer-Assisted Micr. (1998) V10 No. 3, pp 103-111

Schrader M, Bahlmann K, Giese G and Hell SW
4Pi-Confocal Imaging in Fixed Biological Specimens
Biophysical Journal (1998) Volume 75 pp get pdf

Schrader M, Hell SW and van der Voort HTM
Three-dimensional super-resolution with a 4Pi-confocal microscope using image restoration
Journal Of Applied Physics (1998) Volume 84, Number 8 pp get pdf

Sytsma J, Vroom JM, De Grauw CJ and Gerritsen HC
Time-gated fluorescence lifetime imaging and microvolume spectroscopy using two photon excitation
Journal of Microscopy (1998) Vol 191 Pt1 pp 39-51

Verveer PJ (1998)
Computational and Optical Methods for Improving Resolution and Signal Quality in Fluorescence Microscopy. Technische Universiteit Delft. abstract and pdf

Rothen-Rutishauser BM, Messerli MJ, van der Voort H, Guenthert M and Wunderli-Allenspach H
Deconvolution Combined with Digital Colocalization Analysis to Study the Spatial Distribution of Tight and Adherens Junction Proteins
Jo. of Computer-Assisted Micr. (1998) V10 No. 3, pp 103-111

Rothen-Rutishauser BM, Messerli MJ, van der Voort H, Guenthert M and Wunderli-Allenspach, H
Deconvolution Combined with Digital Colocalization Analysis to Study the Spatial Distribution of Tight and Adherens Junction Proteins
Jo. of Computer-Assisted Micr. (1998) V10 No. 3, pp 103-111


Andersson H, Baechi T, Hoechli M, Larsson B, Nievergelt C, Richter C and Swedberg
Autofluorescence as indicator of viability of living cells
XXXIX Symposium of the Society for Histochemistry 24-27 September 1997, Jena, Germany
Histochem Cell Biol (1997) 108 pp 257-285

Hell SW, Schrader M and Van Der Voort HTM
Far-Field fluorescence microscopy with three-dimensional resolution in the 100-nm Range
Journal of Microscopy (1997) Vol 187 Pt1, pp 1-7

Noordmans HJ, Smeulders AWM and van der Voort HTM
Fast volume render techniques for interactive analysis
The Visual Computer (1997) 13 pp 345-358


Kano H, van der Voort HTM, Schrader M, van Kempen GMP and Hell SW
Avalanche photodiode detection with object scanning and image restoration provides 2-4 fold resolution increase in two-photon fluorescence microscopy
Bioimaging 4 (1996) pp 187-197 get pdf

van Kempen GMP, van der Voort HTM and van Vliet LJ
A quantitative comparison of two restoration methods as applied to confocal microscopy
Journal of Microscopy (1997), Volume 185, no. 3, Pages 354 - 365. Image JoM97GKLVea.pdf

Kano H, van der Voort HTM, Schrader M, van Kempen GMP and Hell SW
Avalanche photodiode detection with object scanning and image restoration provides 2-4 fold resolution increase in two-photon fluorescence microscopy
Bioimaging 4 (1996) pp 187-197 get pdf


van der Voort HTM and Brakenhoff GJ
3-D image formation in high-aperture fluorescence confocal microscopy: a numerical analysis
Bioimaging 4 (1996) pp 187-197
Journal of Microscopy, April 1990, Volume 158 (1): 43–54
This paper shows the need to take object orientation into account when segmenting or analyzing microscopic images.