Huygens Multi-Photon Deconvolution Software

Specially designed to take into account the optical properties of all brands of microscopes

RAW Multi-Photon
Huygens Deconvolved
Huygens Multi-Photon Microscopy image deconvolution takes the multiple excitation photons into account, which are simultaneously absorbed by a fluorophore in a fluorescence event. This essential feature in Huygens combined with its state-of-the-art spherical abberation correction, guarantees high image quality and resolution - even of very thick samples.

Image description
Image shows a small region of a complete mouse small intestine organoid (inset) embedded in fluorescent mounting medium (MOWIOL-488 Dabco), and was acquired with a Multi-Photon (NDD) using a low NA 20x water immersion lens. Image kindly provided by Philipp Tripal, Benjamin Schmid, Ralph Palmisano, Optical Imaging Centre Erlangen, Friedrich-Alexander University Erlangen, Germany. scalebar=2 micron

Improve Resolution

Improving image quality and resolution. Learn more: Huygens Deconvolution.

Multiple Algorithms

Use advanced Maximum Likelihood Estimation algorithms. Learn more: Huygens Algorithms.

All Microscope Brands

All brands and types of Multi-Photon Microscopes are supported, as well as many File Formats.


So far Huygens seems to surpass our other products at least for deconvolution and also surface rendering, and most of all, the technical support that you have provided is truly tremendous.

Huong Ha, graduate student Neuroscience Program, Stanford University, USA.
Both my facility users and I are very impressed with the new (and not so new) features, and some of my new users are really enjoying the object analyzer and the ROI options!

Dr. Zoltan Cseresnyes, research associate and co-manager Confocal and 2-Photon Microscopy Core Facility, MDC Berlin, Germany.

Use in research

B. Zandt, A. Losnegård, E. Hodneland et al., Semi-automatic 3D morphological reconstruction of neurons with densely branching morphology: Application to retinal AII amacrine cells imaged with multi-photon excitation microscopy.
Huygens was used to deconvolve multi-photon images with a measured PSF.
J Neurosci Methods 279, 101-118 (2017)

Z. Chen, A. Luciani, J.M. Mateos et al., Transgenic zebrafish modeling low-molecular-weight proteinuria and lysosomal storage diseases.
Huygens was used to deconvolve multi-photon images.
Kidney International 97, 1150-1163 (2020)

For more, see Scientific Publications

Multi-photon microscopy does limited damage to the sample and is therefore often used for imaging live tissues. Unwanted movements can be corrected for with the Huygens Object Stabilizer while movements of interest can be tracked with the Huygens Object Tracker.

Object Stabilizer Object Tracker

More information

Introduction to deconvolution
Huygens Deconvolution
Deconvolution images

SHG signal of the rat soleous muscle's myosin imaged with a Leica two-photon microscope (NA: 1, 20x). Stack has been deconvolved with Huygens using a measured PSF, and visualized with the Huygens SFP Renderer. High quality imaging together with Huygens allows you to see through the muscle's A bands from one side to the other. The stack dimensions is 105x105x87 μm. Image kindly provided by Dr. Dimitris Kapsokalyvas, Department of Molecular Cell Biology, Maastricht University, The Netherlands.

See more: Images in the field of Cell Biology

5th Entry   Pollen Grain Autofluorescence
Autofluorescence from pollen grains was captured with Zeiss 710 NLO multiphoton microscope in three channels using 850nm laser excitation. Two channels (blue and magenta) show two-photon autofluorescence in different spectral ranges, the third channel (green) shows second harmonic generation from starch particles inside the pollen grain. The acquired 3D dataset was deconvolved and maximum intensity projection image was rendered with Huygens. Image kindly provided by Dr. Matyas Molnar, BioVis imaging facility, Uppsala University, Sweden.

See more: Images in the field of Plant Biology

MsEmbryo Developing Vasculature Decon MIP Depth Coded Small
Erythrocytes that mark the developing vasculature in a mouse embryo (E14.5). This 8x11x2mm specimen was imaged on a multiphoton microscope excited at 790nm using a 25x 1.0NA Objective. Subsequently, the image was stitched, deconvolved with Huygens Professional using a measured PSF, and visualised with depth-coded colouring using the Huygens MIP renderer (version 19.10). Image kindly provided by Howard Vindin, Weiss Laboratory, School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Australia.

See more: Images in the field of Genetics and Developmental Biology