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Huygens Array Detector Optical Option

Supports Zeiss LSM780/880 Airyscan®, Fast Airyscan®, nanoSPAD and others!

For information about support for Zeiss LSM980 Airyscan®2 images, please contact support at svi.nl.


Confocal
Huygens Deconvolved superXY

Huygens Array Detector deconvolution option is integrated in a powerful GPU accelerated MLE-algorithm to restore images acquired on multi-detector confocal systems, including the well-received Zeiss® Airyscan (1). Thanks to SVI’s smart multi-detector algorithms, restoration of Array Detector images with Huygens is very efficient. It allows scientists to obtain reliable super-resolution images in which objects at 90nm distance can be distinguished. Importantly, the unique Huygens Array Detector Super-Sampling mode fulfills a wish common to many microscopist as it allows fast image acquisition with less bleaching and photo-toxicity, while improving image resolution and contrast.

Image description:
HeLa cells (MIP) imaged with Zeiss LSM 880 Airyscan system and deconvolved with the Huygens Array detector using the superXY mode. Cells were stained with anti-Ki67 and secondary-Alexa488 (magenta), Phallodin-TMR (White), and anti-alphaTubulin and secondary Abberior Star Red (shown in green). Image kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK).


Reach 90nm resolution

Tests with a range of GattaQuant nanorulers confirm 90nm two-point resolution is achievable.

Unique "Super-sampling"

Huygens Super-sampling mode permits faster imaging without compromising resolution and contrast.

Multiple detectors

Multiple detector layouts are supported, including Zeiss (Fast) Airyscan super-resolution, nanoSPAD detectors, and other generic layouts.


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Testimonials

"We are quite impressed with the Huygens Array Detector results"
Dr. Glyn Nelson, BioImaging Unit, campus for Ageing and Vitality, Newcastle University, UK.

"The new array detector deconvolution has allowed us to improve the resolution of Airyscan images beyond their normal limits. This is particularly useful for resolving small, clustered subcellular organelles."
Dr. Stephen Freeman, Liège Université, GIGA Cell Imaging Platform, Belgium

About array-detector confocal systems

Within array-detector confocal systems, an array of multiple pinhole detectors replaces the conventional pinhole and single detector typical for a regular confocal system. Each detector produces a similar but shifted image as the image from the central pinhole. Summed, the produce an image equivalent to that of a single large pinhole: good signal but no resolution advantage. However, the images are not only shifted w.r.t. each other, but also have a different Point Spread Function. The Huygens MLE algortihm takes all this into account to generate a high resolution, high signal and contrast result.

All Detectors

The Huygens Array Detector Optical Option allows you to obtain high resolution and contrast from array detector data, such as that acquired on a Zeiss® Airyscan system. The Airyscan microscope can be used in 4 modes, and Huygens now offers high quality deconvolution for each single mode:

  • Standard mode: this is the conventional confocal mode of the Airyscan. For Huygens deconvolution you can use the Confocal Optical Option in Huygens setting the pinhole size at the size of the pinhole used during acquisition.
  • Virtual pinhole mode: in this mode you can decide for the size of the pinhole in post-processing. For Huygens deconvolution you can use the Confocal Optical Option in Huygens setting the pinhole size at the size of the pinhole decided in post-processing.
  • Super Resolution mode: these datasets include all 32-detector images. The Array-detector confocal option in Huygens includes various modes to intelligently combine the information from these 32-detector datasets and deconvolve data acquired in Airyscan super-resolution mode.
  • Fast mode: this mode uses elongated laser excitation in combination with 16 detectors (the central 3 'vertical' detector columns). The fast mode allows for the acquisition of 4 lines simultaneously, thereby speeding up the image acquisition with a factor of 4. The Array-detector confocal option in Huygens includes a special Fast Airyscan mode to optimally process the 16-detector datasets from Fast Airyscan.

Next to supporting the various Zeiss Airyscan modes, the Huygens Array Detector Confocal Optical Option also supports other multi-detector layouts. This includes a nanoSPAD detector layout, or other generic mulit-detector layouts.


Reaching 90nm two-point super resolution

The combination of Huygens' unique MLE deconvolution and the information available from the array of detectors, now allow researchers to reach 90nm two-point resolution.
By imaging GATTAquant SIM100B nanorulers, consisting of carefully folded DNA origami with fluorophores spaced at a calibrated distance of 100nm, the Huygens Array detector MLE algorithm is shown to reach a two-point resolving capbility of at least 100nm. Recentely even 90nm nanorulers were resolved resolve 90nm nanorulers.
Nanorulers 100nm
Images obtained with Zeiss Airyscan microscope were kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK




Raw
Huygens Deconvolved superXY

With 80nm sampling in X and Y, you can resolve 140nm GattaQuant (2) nanorulers with the special superXY mode. Shown are MIPs. The right image is a sum of the unprocessed raw data from all detectors. Image obtained with Zeiss Airyscan microscope kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK{

GATTA140B Figure
With 80nm sampling in X and Y, you can resolve 140nm GattaQuant (2) nanorulers with the special superXY mode. Shown are MIPs. Image obtained with Zeiss Airyscan microscope kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK




Fast imaging + Fast high-quality Huygens Super-sampling deconvolution

A particularly useful property of array-detector systems, is that the Nyquist criterion is much less strict as compared to confocal. This allows for imaging at larger pixel sizes and thus faster image acquisition. As a consequence, images will be less affected by photo-bleaching and/or can include a much larger field of view than if acquired with a confocal setup. Deconvolution of Array detector data can be performed with the specifically designed Huygens Super-sampling mode without any trade-off in SNR and while keeping the super-resolution aspect.
In 2016, Carl Zeiss® introduced the Fast Airyscan mode, with which 4 lines can be scanned simultaneously. Huygens also offers deconvolution for these Fast Airyscan images.
In the Airyscan Fast mode, 4 lines can be scanned simultanously by adjusting the excitation profile, and by using 16-detectors in the most central columns in the detector Array. The image acquisition can thus be sped up with an additional factor of 4 when imaging in fast-scan mode. Huygens also offers a super-resolution deconvolution mode for Fast Airyscan images, as it can optimally combine the information from all available detectors, to generate a super-resolution result image of unprecedented quality for confocal based systems.


Now Available for Huygens Essential, Professional and Core

The new Huygens option supports various Zeiss Airyscan modes (confocal, virtual pinhole, super-res Airyscan, Fast-mode) and nanoSPAD detectors in combination with multi-channel z-stacks and time-series. The product is commercially available as a licensed option for Huygens Essential, Professional and Core.

Why wait? Try out this new option by downloading Huygens and request a test license, or receive pricing information.

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1. Carl Zeiss Microscopy GmbH.
2. GATTAquant GmbH. For more information, see the GATTAquant website.