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Huygens Array Detector Optical Option

Supports Zeiss LSM780/880 Airyscan®, Fast Airyscan®, LSM900/980 Airyscan® SR, nanoSPAD and others!

For more information about support for Zeiss Airyscan® images, please contact support at svi.nl.





Confocal
Huygens Deconvolved superXY

Huygens Array Detector deconvolution is an integrated approach to restore and deconvolve array detector data with superior results. It works on data acquired on any multi-detector confocal system, such as the well-received Zeiss® Airyscan 1 and 2 (SR). Different from reconstruction based on pixel reassignment, as often performed by the instrument acquisition software, Huygens’ smart multi-detector algorithms deconvolve the raw data directly and correct common artefacts. As such, Huygens allows scientists to obtain reliable super-resolution images in which objects at 90nm distance can be distinguished.

All our algorithms are MLE-based and GPU accelerated. Importantly, the unique Huygens Array Detector Super-Sampling mode allows for fast image acquisition with less bleaching and photo-toxicity, while improving image resolution and contrast.

Image description
HeLa cells (MIP) imaged with Zeiss LSM 880 Airyscan system and deconvolved with the Huygens Array detector using the superXY mode. Cells were stained with anti-Ki67 and secondary-Alexa488 (magenta), Phallodin-TMR (White), and anti-alphaTubulin and secondary Abberior Star Red (shown in green). Image kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK).


Reach 90nm resolution

Tests with a range of GattaQuant nanorulers confirm 90nm two-point resolution is achievable.


Unique Super-sampling

Huygens Super-sampling mode permits faster imaging without compromising resolution and contrast.


Multiple detectors

Multiple detector layouts are supported, including Zeiss (Fast) Airyscan super-resolution, nanoSPAD detectors, and other generic layouts.




Testimonials

We are quite impressed with the Huygens Array Detector results

Dr. Glyn Nelson, microscopist in the Bioimaging Unit, Campus for Ageing and Vitality, Newcastle University, UK.
The new array detector deconvolution has allowed us to improve the resolution of Airyscan images beyond their normal limits. This is particularly useful for resolving small, clustered subcellular organelles.

Dr. Stephen Freeman, light microscopy platform expert at GIGA Liege, Liège Université, Belgium.


Reaching 90nm two-point super resolution

The combination of Huygens' unique MLE deconvolution and the information available from the array of detectors, now allow researchers to reach 90nm two-point resolution.
By imaging GATTAquant SIM100B nanorulers, consisting of carefully folded DNA origami with fluorophores spaced at a calibrated distance of 100nm, the Huygens Array detector MLE algorithm is shown to reach a two-point resolving capbility of at least 100nm. Recently even 90nm nanorulers were resolved resolve 90nm nanorulers.
Nanorulers 100nm
Images obtained with Zeiss Airyscan microscope were kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK


Fast imaging + Fast high-quality Huygens Super-sampling deconvolution

A particularly useful property of array-detector systems, is that the Nyquist criterion is much less strict as compared to confocal. This allows for imaging at larger pixel sizes and thus faster image acquisition. As a consequence, images will be less affected by photo-bleaching and/or can include a much larger field of view than if acquired with a confocal setup. Deconvolution of Array detector data can be performed with the specifically designed Huygens Super-sampling mode without any trade-off in SNR and while keeping the super-resolution aspect.

In the Airyscan Fast mode, 4 lines can be scanned simultanously by adjusting the excitation profile, and by using 16-detectors in the most central columns in the detector Array. The image acquisition can thus be sped up with an additional factor of 4 when imaging in fast-scan mode. Huygens also offers a super-resolution deconvolution mode for Fast Airyscan images, as it can optimally combine the information from all available detectors, to generate a super-resolution result image of unprecedented quality for confocal based systems.


Use in research

Mostafa F. ElMaghraby, Peter Refsing Andersen, Florian Pühringer et al., A Heterochromatin-Specific RNA Export Pathway Facilitates piRNA Production.
Airyscan images were deconvolved with Huygens.
Cell 178, 964-979 (2019)

For more, see Scientific Publications

Because of its fast imaging and low phototoxicity and bleaching, Array Detector microscopy is well fit for live-cell imaging. Huygens can then be used to stabilize time series and to track object movement over time.

Object Stabilizer Object Tracker

More information

Introduction to deconvolution
Huygens Deconvolution software
Deconvolution images



Raw
Huygens Deconvolved superXY
With 80nm sampling in X and Y, you can resolve 140nm GattaQuant nanorulers with the special superXY mode. Shown are MIPs. The right image is a sum of the unprocessed raw data from all detectors. Image obtained with Zeiss Airyscan microscope kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK

GATTA140B Figure
With 80nm sampling in X and Y, you can resolve 140nm GattaQuant nanorulers with the special superXY mode. Shown are MIPs. Image obtained with Zeiss Airyscan microscope kindly provided by Dr. Christoffer Lagerholm (Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of Oxford, UK