Huygens STED Deconvolution Software

Specially designed for improving all STED images

STED microscopy has proven to be a valuable super-resolution technique for resolving objects smaller than the diffraction-limit, and Huygens leading STED deconvolution pushes this even further. All types of STED (STimulated Emission Depletion microscopy) images can be deconvolved with Huygens succesfully. Huygens unsurpassed STED deconvolution offers two-fold improvements in X, Y, and Z resolution and boosts contrast with SNRs increasing by a factor of eight. A FWHM resolution of 22nm has been achieved. More in this publication.

Image description
Primary hippocampal neurons with cytoskeleton proteins labelled (magenta, alpha-Adducin, Abberior STAR 635P and green, ßII spectrin, Alexa 594). Imaged with Abberior Instruments’ STEDYCON, and deconvolved using Huygens STEDYCON setting.

Reach 22nm Resolution

Improve image resolution, contrast and SNR effectively. Learn more: Huygens Deconvolution.

Most advanced Algorithms

All Microscope Brands

All STED systems (incl. 3X) and their File Formats are supported.


I have been a Huygens user for 8-9 years now and the support provided by you guys are fantastic!

Dr. Priyam Banerjee, image analysis researcher and instructor, MD Anderson Cancer Center, USA.
I've been working with Huygens for couple of years now, and I can say that it's a really powerful tool. SVI have done a great job at developing a software package that is both flexible and user friendly, which is not always easy when it comes to scientific data processing.

Dr. Stefan Stanciu, researcher at the Center for Microscopy-Microanalysis and Information Processing, UPB, Romania.

Use in research

Watanabe K, Takao D, Ito KK et al., The Cep57-pericentrin module organizes PCM expansion and centriole engagement.
STED images were deconvolved with Huygens.
Nature Communications 10 (2019)

Spahn C, Grimm JB, Lavis LD et al., Whole-Cell, 3D, and Multicolor STED Imaging with Exchangeable Fluorophores.
STED images were deconvolved with Huygens.
Nano Letters 19, 500-505 (2019)

For more, see Scientific Publications

STED 3D stacks often suffer from misalignments due to thermal fluctuations. We advise to use the Huygens Object Stabilizer to correct the raw data before STED deconvolution. Thermal drift correction is also integrated into the specialized STED option for the Huygens PSF Distiller.

Object Stabilizer PSF Distiller

More information

Introduction to deconvolution
Huygens Deconvolution software
Deconvolution images

Raw Confocal
STED + Huygens Deconvolved
Centrosome linker. U2OS cells in which the centrosome-linker-protein rootletin was immunolabelled using secondary antibodies coupled to Abberior STAR RED. Sample was prepared by R. Vlijm at MPI for Medical Research, Heidelberg, Germany. Imaged with Abberior Instruments’ STEDYCON and deconvolved with Huygens Professional.

See more: Images in the field of Cell Biology

Gated STED single plane image and confocal data of a centriole stained with primary and secondary antibody conjugated to Chromeo488. Raw and Huygens deconvolved images are shown. The intensity profile was fitted to Lorentz distribution. Image kindly provided by Dr. Grazvydas Lukinavicius, EPFL, Lausanne, Switzerland.

See more: Images in the field of Cell Biology

Above: Single XY plane of pulsed-STED data before (left) and after deconvolution (right) of the complete z-stack with Huygens. Structure (240 nm ring diameter) was stained with primary and secondary antibody conjugated to Alexa488. Below: Axial MIP projection of the complete STED z-stack, before (left) and after deconvolution with Huygens (right). Image kindly provided by Dr. Grazvydas Lukinavicius, EPFL, Lausanne, Switzerland.

See more: Images in the field of Cell Biology

Pre-synaptic marker (Atto647n on 2nd antibody) within the neuromuscular junction of Drosophila melanogaster larvae shows holes in flower structures that were deconvolved with Huygens (picture in the middle), and that can not be identified in the raw Leica STED image (left). Raw pulsed-STED image was first stabilized and then deconvolved with Huygens. Image kindly provided by Oliver Kobler, Ulrich Thomas and Werner Zuschratter, CNI, Leibniz Institute Magdeburg, Germany.

See more: Images in the field of Neurosciences

Confocal and STED image of chromosomes with histon proteins before and after deconvolution. Combined image of chromosomes (confocal in green) and histon protein (STED in red) image before and after deconvolution. More contrast and resolution can be observed after deconvolution of both the Leica confocal and STED channel. The image shows an enlargement of the red channel. Image kindly provided by Dr. Juraj Kabat, Biological Imaging Facility, NIH/NIAID, Bethesda, USA.

See more: Images in the field of Cell Biology

This Huygens deconvolved image shows a IsoSTED image of tubulin labelled with a small molecule probe, and imaged on an Abberior STED expert line microscope. Confocal and 3D STED images were deconvolved using Huygens software. Movie generated using Huygens MIP renderer. Image kindly provided by Dr. Grazvydas Lukinavicius, MPI for Biophysical Chemistry - Department of NanoBiophotonics, Max Planck Institute, Germany.

See more: Images in the field of Cell Biology