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Huygens Stitcher

High-quality stitching for all microscopy data types with optional deconvolution and vignetting correction.



Equipped with a unique xyz tile positioning approach, Huygens Sticher fits each recorded tile with extremely high 3D precision into the mosaic. With intelligent and automated vignetting correction, seamless stitching is ensured. The easy-to-use Wizard combines fast GPU-accelerated fitting and vignetting correction with deconvolution to achieve superior results.

Image description:
Dendritic spines even visible in a large FOV (field of view). Tiles from a 2-channel Zeiss confocal .czi file of a brain section were stitched, automatically vignetting-corrected, and deconvolved with Huygens Stitcher. Image courtesy Dr. Moritz Kirschmann, Center for Microscopy and Image Analysis ZMB, University of Zurich, Switzerland.

Image

No Limits

No limit to tile number, file size, and image dimensions. Works images from all types of microscopes.

Vignetting correction

Intelligent automated and manual vignetting correction available.

Deconvolution Included

Resolution and contrast improvement using the wizard-integrated Huygens renowned deconvolution.


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Features

  • Easy to use Wizard for obtaining unmatched stitching results.
  • Interactive mosaic preview of the tile content.
  • Optimal 3D positioning of each tile guarantees smooth transitions across regions of overlap in X, Y and Z.
  • Tiles with 0% overlap can still be stitched.
  • Support for 2D, 3D, multichannel, and time series images.
  • No limit to the number of tiles that can be stitched.
  • No limit to the total file size of the input sequence.
  • Multiple scenes with tiles can be stitched all at once.
  • Automatic vignetting estimation and correction option for each channel.
  • Manual vignetting and shading correction option with a recorded flat and dark-filed frame.
  • Optional high quality Huygens deconvolution integrated in workflow.
  • Registration and deconvolution is supported by GPU.
  • Choice for only stitching and optional vignetting also possible by simply skipping deconvolution in this wizard.
  • Stitches all file types supported in Huygens (some my require a XML file with layout/location information).
  • Reads layout information from Abberior STEDYCON (OBF), DeltaVision, LIF, CZI, ND2(Windows) formats automatically
  • Support for custom stitching patterns.

No Vignetting Correction
Auto Vignetting correction
Huygens stitched tiles of widefield (Leica) fluorescent Leica LIF data. The same data is shown with and without Huygens automatic vignetting and shading correction. Image represents a developing mouse cortex (P30) stained for Tbr1 and reelin. Courtesy of Dr. Kolk and Dr. Witteveen, Nijmegen Centre for Molecular Life Science, The Netherlands

inset: Huygens Deconvolved
inset: RAW confocal
Impressive large FOV (22477 x 19936 pixels) dual-channel confocal dataset, imaged with the Abberior Instruments STEDYCON. The dataset was acquired by imaging 3090 individual tiles. All tiles were stitched using the Huygens Stitcher wizard, resulting in a field of view of approx 2 x 2 millimeter with 100nm pixel sampling. Inset shows a zoomed-in region of single cell to appreciate the level of detail in the full dataset.


Available for Huygens Essential & Professional

The Huygens Stitcher can be tested with a free test license. The product is commercially available as a licensed option for Huygens Essential & Professional.

Why wait? Try out this option by downloading Huygens and request a test license, or receive pricing information.

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Upcoming Events

Wed 30 of Sep, 2020 00:00 CEST
SVI Huygens imaging Course in 2021