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Scientific Volume Imaging B.V.

Scientific Volume Imaging

Deconvolution - Visualization - Analysis


™Huygens Stitcher

High-quality stitching of TBs without artefacts for all microscopy data types

Huygens Stitcher and Stitcher Express read tile images and associated metadata from any microscope type. This includes tile position information which eliminates the need for an extra file conversion step. Its unique XY and Z tile positioning method ensures high 3D precision. The latest Stitcher Express ensures fast and optimal stitching of any image file that includes metadata. Both Express and the Stitcher Wizard combine fast GPU-accelerated tile fitting, vignetting correction, and Huygens renowned deconvolution, enabling efficient and high-quality results.

With the recent Huygens Resolution Pyramid file format functionality, the Stitcher can handle datasets of Terabytes with ease. Batch stitching can be performed within the Workflow Processor. Final results can be saved in (open-source) formats that also preserve the original image metadata.



Fast stitching with Express function (since Huygens 26.10)

No file conversion required

Advanced algorithms to ensure the best possible overlap

Integrated deconvolution

Stitch terabytes of data with Resolution Pyramids

Image
Image description Dendritic spines are visible even in a large field of view. Confocal image tiles of a brain section were stitched, automatically vignetting-corrected and deconvolved with Huygens Stitcher. Courtesy of Dr. Moritz Kirschmann, University of Zurich, Switzerland.


No size limits

No limit to number of tiles, file size, or image dimensions.

Vignetting and overlap correction

Intuitive manual and intelligent automated vignetting correction available.

Improved by deconvolution

Integrated Huygens Deconvolution boosts stitching performance and image quality.



Testimonials


Huygens Stitcher works like a charm and the horrendous shading / vignetting is rescued flawlessly!


Dr. Arnaud Gaudin,
Senior microscopy services officer at the QBI, University of Queensland, Australia.

I am stitching and deconvolving 600 2Ch-xy tiles (total size 160GB) and everything seems to work fine. Positioning of tiles happened using GPU, as well as deconvolving. Everything seems nice!


Dr. Ioannis Alexopoulos,
Light microscopy and image analysis specialist, Radboud University, The Netherlands.

The Huygens Stitching Wizard did a fantastic job of stitching a 850GB tiles image using virtual RAM, and I have been unable to find any flaws in X, Y, or Z.


Robert N. Wine,
Fluorescence Microscopy Imaging Center, NIH - National Institute of Environmental Health Sciences, United States.

We are extremely happy with stitching a 3TB dataset in a day!


Bálint Tamás,
Foldy group, Brain Research Institute, Zürich, Switzerland.



Features


  • Easy to use and stable Wizard for obtaining unmatched stitching results.
  • Interactive mosaic preview of the tile content.
  • Optimal 3D positioning of each tile guarantees smooth transitions across regions of overlap in X, Y and Z.
  • Tiles with 0% overlap can still be stitched.
  • Support for 2D, 3D, multichannel, and time series images.
  • No limit to the number of tiles that can be stitched.
  • No limit to the total file size of the input sequence.
  • Multiple scenes with tiles can be stitched all at once.
  • Automatic vignetting estimation and correction option for each channel.
  • Manual vignetting and shading correction option with a recorded flat-field and dark frame.
  • Optional high quality Huygens deconvolution integrated in workflow.
  • Choice for only stitching and optional vignetting also possible by simply skipping deconvolution in this wizard.
  • Registration and deconvolution is supported by GPU.
  • Stitches all file types supported in Huygens (some my require a XML file with layout/location information).
  • Reads layout information from Abberior STEDYCON (OBF), DeltaVision, LIF, CZI, ND2(Windows) formats automatically.
  • Support for custom stitching patterns.
  • Settings can be saved to a template for repetitive use and for batch stitching in the Workflow Processor.
  • Direct saving to multi-resolution Pyramid file format for images that do not fit in RAM (requires Huygens Professional)

With Huygens GPU Acceleration, stitching and deconvolution are extremely fast so that you do not need to compromise between speed and file size.

GPU Acceleration

More information

Vignetting correction
Resolution Pyramid
Support page
Stitcher webinar


No Vignetting Correction
Auto Vignetting correction
Huygens stitched tiles of widefield (Leica) fluorescent Leica LIF data. The same data is shown with and without Huygens automatic vignetting and shading correction. Image represents a developing mouse cortex (P30) stained for Tbr1 and reelin.
inset: Huygens Deconvolved
inset: RAW confocal
Impressive large FOV (22477 x 19936 pixels) dual-channel confocal dataset, imaged with the Abberior Instruments STEDYCON. The dataset was acquired by imaging 3090 individual tiles. All tiles were stitched using the Huygens Stitcher wizard, resulting in a field of view of approx 2 x 2 millimeter with 100nm pixel sampling. Inset shows a zoomed-in region of single cell to appreciate the level of detail in the full dataset.