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Huygens Everywhere

Help Center




Manual

Manuals

Detailed information on each Huygens option clearly presented.

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Imaging Academy

Huygens Imaging Academy

Learn all about getting the best images and the most reliable results for your publications.

Visit the Imaging Academy >

Webinar

Webinars

All Huygens options introduced and explained in Webinar videos.

Watch Webinars >



FAQ


Installation and Use

Yes. To test Huygens Everywhere, please download Huygens and request a login for a free trial valid for 14 days. Request trail: Huygens Essential & Huygens Localizer
Absolutely, you can use your Huygens login account on any computer (Windows, Mac OS X and Linux) with web access that has Huygens installed from Download.
Huygens is available for Windows, Mac OS X and Linux. It is advised to have at least 4GB RAM and 2 CPU cores in your computer. Huygens advanced 3D visualization does not need an advanced graphics card. Although not required, GPU acceleration does significantly improve the computational performance. See also Available Platforms
The icons represent the Huygens basics. These basics and their included options can be specifically activated with the appropriate account or license that you have received from us.
Huygens Everywhere is currently available for Huygens Essential and Huygens Localizer. Much of the functionality of Huygens Professional is also present in Essential, so for the moment we advice you to use Huygens Essential.
We advice you to look at our multi-user access mode Huygens Floating, which is perfectly suited for your needs. Learn more: Huygens Floating License


Subscription & Account

Of course! Each Huygens Everywhere is personal allowing one user at the time on any computer with Huygens installed. So your colleagues can also get their own personal Huygens Everywhere fitting their specific research.
Yes, your subscription will be automatically prolonged. You can stop your subscription at any time after the 1st year.
Yes this is possible. A restart will require a one year subscription.
After logging in on the SVI website, click on your username in the top right. Under "preferences" you can change your email address and/or password.
Yes. You can register again at svi.nl and create a new user account. Beware that your current Huygens Everywhere credentials then needs updating. For activating your new account, please contact mailto:sales@svi.nl and mention your old and new username.
When registering for the Huygens website you are asked to give a username and your email address. You can access with both the Huygens website. You can log in to Huygens Everywhere only with your username. So if your username and email address are the same you can access the software with it as well. If you use another username please use that. If you want to view or change your email address and/or password: login on the SVI website, click on your username in the top right, select "preferences" and change if necessary.


Microscopy images

Huygens Everywhere reads with high precision nearly all mainstream microscopy file types. See File Formats for all imported and exported file types.
The best file format for saving image to is ICS2, because this format is also used by many other software packages , does not compromise data quality, and stores the image metadata and dimensions correctly. Also HDF5and OME are widely used. See File formats (https://svi.nl/fileformats).
Various parameters should be taken into account when acquiring images. The two important ones being sampling and clipping. Consult our Nyquist Calculator for the best possible sampling advise and clipping can be prevented by avoiding too high laser intensities. See also: Acquisition Pitfalls.
Huygens can recognize and correct a range of typical imaging issues. You can read more at the Huygens Imaging Academy. For direct information on image acquisition, see this webpage: Acquisition Pitfalls.


How to cite Huygens

To cite Huygens, please describe the Huygens package and version you used. It is also recommended to descibe the used settings. For example: "All images were deconvolved with Huygens Professional version 20.04 (Scientific Volume Imaging, The Netherlands, http://svi.nl), using the CMLE algorithm, with SNR:20 and 40 iterations". See also How to Cite Huygens

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