Virtual SVI Huygens Workshop
April 14th and 15th, 2021
Get the best out of your microscopy image
With the SVI Huygens software you can unlock the true potential of your microscopy images. During our virtual workshop we will guide you through our specialized features for restoring, visualizing and analyzing microscopy data. Using a collection of demo images, you will get hands-on experience with our world-renowned software. Similar courses, previously held at the SVI headquarters, were rated very good to excellent by the participants.
Program interactive workshop April 2021
Day 1 (Wednesday): Deconvolution, data handling and quantification
(videocall is active from 13:30)
13.50 - 14:00 : Welcome
14:00 - 14:30 : Introduction by Hans van der Voort (founder of SVI)General introduction to microscopy image Formation, Deconvolution and Restoration
- Light/Wave properties, Spatial frequencies
- Deconvolution algorithms
- Point spread function - Optical Transfer Function
- Super-resolution imaging
14:30 - 14:40 : Deconvolution - Visualization - Analysis : A piece of cakeDemo of Huygens with an example image.
14:40 - 14:50 : Huygens supports images from Widefield to Super-resolutionExample datasets from widefield to super-resolution data of STED, Airyscan and SMLM.
14:50 - 15:15 : Get started with HuygensOpening and converting images in Huygens, microscopy parameters and metadata handling, clipping, undersampling, viewing and inspecting the image, identifying possible issues.
15:15 - 15:30 : Break
15:30 - 16:10 : Deconvolution Wizard and Twin SlicerUse the Deconvolution Wizard to obtain optimal results, inspect and compare image using the Twin Slicer.
16:10 - 16:30 : Batch processing with the Workflow ProcessorHow to design and schedule multiple deconvolution tasks with the Workflow Processor.
16:30 - 16:45: Huygens Everywhere, remote display, web-based with multiple peopleWhether you are a single user or with many, a beginner or an expert, at home or in the office: Huygens offers a solution for all situations!
16:45 - 17:00 : Break
17:00 - 17:25 : Deconvolution and Reliable quantification of image dataOptimal Deconvolution settings, image/signal quantification, reproducible results.
17:25 - 17.45 : Huygens automated PSF generation and PSF distillationHow to distill an experimental PSF from bead images, create a theoretical PSF, and measure PSF quality.
17:45 - 18:00 : Discussion - questions
Day 2 (Thursday): Restoration, visualization and analysis
13.45 - 14:00 : Welcome - start videocall
14:00 - 14:05 : Recap day 1
14:05 - 14:20 : Localizer - SMLM imagingAnalysis of 2D/3D single molecule localization microscopy data. Fast, accurate and easy with Huygens Localizer.
14:20 - 14:40 : Light Sheet Deconvolution & FusionFuse and deconvolve all your light Sheet data with Huygens Fuser.
14:45 - 15:05 : Tile StitchingStitching, automated/manual vignetting correction, and deconvolution combined in one Wizard.
15:05 - 15:20 : Break
15:15 - 15.45 : Colocalization AnalyzerMeasure colocalization coefficients and visualize them using Huygens' unique 3D colocalization map.
15:45 - 15:55 : Acquisition issues and how to correct themHow to deal with image distortions and acquisition pitfalls. Addressed issues include noise, blurring, bleaching, hotpixels, spherical aberration, bleedthrough, drift.
15:55 - 16:10 : Chromatic aberration correctionChromatic and spherical aberration have a significant impact on image quality and analysis. In this session you will learn how to identify and correct these issues in Huygens.
16:10 - 16:25 : CrosstalkCrosstalk can be disastrous for image visualization and analysis. Huygens helps you with detect and correct crosstalk.
16:25 - 16:45 : Object/image stabilizationMeasure and correct drift and rotation in space and time with Huygens Object Stabilizer.
16:45 - 17:00 : Break
17:00 - 17:20 : Object AnalyzerSegment and analyze objects within your image using Huygens Object Analyzer.
17:20 - 17:30 : Object TrackingAutomated object recognition & tracking with machine based learning and track analysis using Huygens Object Tracker.
17:30 - 17:50 : Quiz - Apply what you learnedGet the best out of a microscopy dataset. Use Huygens optimally to identify and correct imaging issues in a demo image.