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Virtual SVI Huygens Workshop

April 14th and 15th, 2021



Get the best out of your microscopy image

With the SVI Huygens software you can unlock the true potential of your microscopy images. During our virtual workshop we will guide you through our specialized features for restoring, visualizing and analyzing microscopy data. Using a collection of demo images, you will get hands-on experience with our world-renowned software. Similar courses, previously held at the SVI headquarters, were rated very good to excellent by the participants.


Workshop4

Program interactive workshop April 2021



Day 1 (Wednesday): Deconvolution, data handling and quantification


(videocall is active from 13:30)

13.50 - 14:00 : Welcome

14:00 - 14:30 : Introduction by Hans van der Voort (founder of SVI)

General introduction to microscopy image Formation, Deconvolution and Restoration
  • Light/Wave properties, Spatial frequencies
  • Deconvolution algorithms
  • Point spread function - Optical Transfer Function
  • Super-resolution imaging

14:30 - 14:40 : Deconvolution - Visualization - Analysis : A piece of cake

Demo of Huygens with an example image.
HANDS-ON

14:40 - 14:50 : Huygens supports images from Widefield to Super-resolution

Example datasets from widefield to super-resolution data of STED, Airyscan and SMLM.
DEMO

14:50 - 15:15 : Get started with Huygens

Opening and converting images in Huygens, microscopy parameters and metadata handling, clipping, undersampling, viewing and inspecting the image, identifying possible issues.
HANDS-ON

15:15 - 15:30 : Break


15:30 - 16:10 : Deconvolution Wizard and Twin Slicer

Use the Deconvolution Wizard to obtain optimal results, inspect and compare image using the Twin Slicer.
HANDS-ON

16:10 - 16:30 : Batch processing with the Workflow Processor

How to design and schedule multiple deconvolution tasks with the Workflow Processor.
HANDS-ON

16:30 - 16:45: Huygens Everywhere, remote display, web-based with multiple people

Whether you are a single user or with many, a beginner or an expert, at home or in the office: Huygens offers a solution for all situations!

16:45 - 17:00 : Break


17:00 - 17:25 : Deconvolution and Reliable quantification of image data

Optimal Deconvolution settings, image/signal quantification, reproducible results.

17:25 - 17.45 : Huygens automated PSF generation and PSF distillation

How to distill an experimental PSF from bead images, create a theoretical PSF, and measure PSF quality.
HANDS-ON

17:45 - 18:00 : Discussion - questions





Day 2 (Thursday): Restoration, visualization and analysis


13.45 - 14:00 : Welcome - start videocall


14:00 - 14:05 : Recap day 1


14:05 - 14:20 : Localizer - SMLM imaging

Analysis of 2D/3D single molecule localization microscopy data. Fast, accurate and easy with Huygens Localizer.
HANDS-ON

14:20 - 14:40 : Light Sheet Deconvolution & Fusion

Fuse and deconvolve all your light Sheet data with Huygens Fuser.
DEMO

14:45 - 15:05 : Tile Stitching

Stitching, automated/manual vignetting correction, and deconvolution combined in one Wizard.
HANDS-ON

15:05 - 15:20 : Break


15:15 - 15.45 : Colocalization Analyzer

Measure colocalization coefficients and visualize them using Huygens' unique 3D colocalization map.
HANDS-ON

15:45 - 15:55 : Acquisition issues and how to correct them

How to deal with image distortions and acquisition pitfalls. Addressed issues include noise, blurring, bleaching, hotpixels, spherical aberration, bleedthrough, drift.

15:55 - 16:10 : Chromatic aberration correction

Chromatic and spherical aberration have a significant impact on image quality and analysis. In this session you will learn how to identify and correct these issues in Huygens.
HANDS-ON

16:10 - 16:25 : Crosstalk

Crosstalk can be disastrous for image visualization and analysis. Huygens helps you with detect and correct crosstalk.
HANDS-ON

16:25 - 16:45 : Object/image stabilization

Measure and correct drift and rotation in space and time with Huygens Object Stabilizer.
HANDS-ON

16:45 - 17:00 : Break


17:00 - 17:20 : Object Analyzer

Segment and analyze objects within your image using Huygens Object Analyzer.
HANDS-ON

17:20 - 17:30 : Object Tracking

Automated object recognition & tracking with machine based learning and track analysis using Huygens Object Tracker.
DEMO

17:30 - 17:50 : Quiz - Apply what you learned

Get the best out of a microscopy dataset. Use Huygens optimally to identify and correct imaging issues in a demo image.
HANDS-ON

17:50 - 18:00 : Q&A, closing remarks and farewell