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Free Virtual SVI Huygens Imaging Workshop

April 25th and 26th, 2023 (EDT/PDT/CEST, New York/Los Angeles/Amsterdam)





Stay true to your imaged object!

Restore your valuable microscopy images & visualize and analyze them in the most reliable manner with Huygens. This free virtual workshop consists of lectures with in-depth information on various imaging topics, and demo/handson sessions with the Huygens Software. You will learn how to acquire the best possible microscopy data and how to use the specialized Huygens features for restoring, visualizing and analyzing your images. More than 96.4% of the attendents of the previous workshop were (very) satisfied. Click here to see some reviews.



Banner Workshop April2023 1


Preliminary Program interactive workshop


Date: Tuesday (25th) and Wednesday (26st), Time:
New York: 9:00 - 13:00 EDT
Los Angeles: 6:00 - 10:00 PDT
Rio de Janeiro: 10:00 - 14:00 BRT
London: 14:00pm - 18:00 BST
Amsterdam: 15:00 - 19:00 CEST
Bucharest: 16:00 - 20:00 EEST
See here how your day and time corresponds to 15:00 CEST.



Day 1 (Tuesday): Deconvolution, data handling and quantification


All times are displayed in Central European Summer Time (CEST, Amsterdam).

14.50 - 15:00 AM : Welcome


15:00 - 15:30 : Introduction


15:30 - 15:40 : Deconvolution - Visualization - Analysis : A piece of cake

Full-scale demo of deconvolution and analysis
HANDS-ON

15:40 - 15:50 : Huygens for all Widefield to Super-resolution images

Example data from customers of widefield to super-resolution microscopes, including STED, Airyscan and SMLM.
DEMO

15:50 - 16:15 : Get started with Huygens

Opening and converting images in Huygens, microscopy parameters and metadata handling, clipping, undersampling, viewing and inspecting the image, identifying possible issues.
HANDS-ON

16:15 - 16:25 : Break


16:25 - 16:35 : Deconvolution Express and Batch Feeder

Fully automated deconvolution and batch processing on the fly
HANDS-ON

16:35 - 17:15 : Deconvolution Wizard and Twin Slicer

Use the Deconvolution Wizard to obtain optimal results, inspect and compare image using the Twin Slicer.
HANDS-ON

17:15 - 17:40 : Batch processing with the Workflow Processor

How to design and schedule multiple deconvolution and restoration tasks with the Workflow Processor.
HANDS-ON

17:40 - 17:50 : Huygens Everywhere, remote display, web-based with multiple people

Whether you are a single user or with many, a beginner or an expert, at home or in the office: Huygens offers a solution for all situations!

17:50 - 18:05 : Break


18:05 - 18:20 : True deconvolution and Reliable quantification of image data

Optimal Deconvolution settings, image/signal quantification, reproducible results.

18:20 - 18:40 : Super-resolution images and Huygens, incl PSF distillation

How to treat super-resolution images, and how to distill an experimental PSF from bead images, Microscope Quality Control, Compare experimental and theoretical PSFs easily
HANDS-ON

18:45 - 18:55 : Discussion - questions


18:55 - 19:00 Closing remarks - End of Day 1





Day 2 (Wednesday): Restoration, visualization and analysis


14.50 - 15:00 AM: Welcome - start videocall

See here how your day and time corresponds to 15:00 CEST.

15:00 - 15:05 : Recap day 1


15:05 - 15:20 : Localizer - SMLM and Cluster Analysis

Analysis of 2D/3D single molecule localization microscopy data. Easy, Fast, and accurate with Huygens Localizer.
Handson

15:20 - 15:40 : Light Sheet Deconvolution & Fusion

Fuse and deconvolve all your multiview Light Sheet data with Huygens Fuser.
DEMO

15:40 - 16:00 : Tile Stitching

Stitching, automated/manual vignetting correction, and deconvolution combined in one Wizard.
HANDS-ON

16:00 - 16:10 : Break


16:10 - 16:45 : Colocalization Analyzer

Measure colocalization coefficients and visualize them using Huygens' unique 3D colocalization map.
HANDS-ON

16:45 - 16:55 : Acquisition issues and how to correct them

How to deal with image distortions and acquisition pitfalls. Addressed issues include noise, blurring, bleaching, hotpixels, spherical aberration, bleedthrough, drift.

16:55 - 17:15 : Chromatic aberration correction

Chromatic and spherical aberration have a significant impact on image quality and analysis. In this session you will learn how to identify and correct these issues in Huygens.
HANDS-ON

17:15 - 17:25 : Crosstalk

Crosstalk can be disastrous for image visualization and analysis. Huygens helps you with detect and correct crosstalk.
HANDS-ON

17:25 - 17:40 : Object/image stabilization

Measure and correct drift and rotation in space and time with Huygens Object Stabilizer.
HANDS-ON

17:40 - 17:55 : Break


17:55 - 18:20 : Object Analyzer

Segment and analyze objects within your image using Huygens Object Analyzer.
HANDS-ON

18:20 - 18:30 : Object Tracking

Automated object recognition & tracking with machine based learning and track analysis using Huygens Object Tracker.
DEMO

18:30 - 18:50 : Quiz - Apply what you learned

Use Huygens optimally to identify and correct imaging issues in a demo image.
HANDS-ON

18:50 - 19:00 : Q&A, closing remarks and virtual farewell drink