Free Virtual SVI Huygens Imaging Workshop
April 25th and 26th, 2023 (EDT/PDT/CEST, New York/Los Angeles/Amsterdam)
Stay true to your imaged object!
Restore your valuable microscopy images & visualize and analyze them in the most reliable manner with Huygens. This free virtual workshop consists of lectures with in-depth information on various imaging topics, and demo/handson sessions with the Huygens Software. You will learn how to acquire the best possible microscopy data and how to use the specialized Huygens features for restoring, visualizing and analyzing your images. More than 96.4% of the attendents of the previous workshop were (very) satisfied. Click here to see some reviews.
Preliminary Program interactive workshop
Date: Tuesday (25th) and Wednesday (26st), Time:
New York: 9:00 - 13:00 EDT
Los Angeles: 6:00 - 10:00 PDT
Rio de Janeiro: 10:00 - 14:00 BRT
London: 14:00pm - 18:00 BST
Amsterdam: 15:00 - 19:00 CEST
Bucharest: 16:00 - 20:00 EEST
See here how your day and time corresponds to 15:00 CEST.
Day 1 (Tuesday): Deconvolution, data handling and quantification
All times are displayed in Central European Summer Time (CEST, Amsterdam).
14.50 - 15:00 AM : Welcome
15:00 - 15:30 : Introduction
15:30 - 15:40 : Deconvolution - Visualization - Analysis : A piece of cakeFull-scale demo of deconvolution and analysis
15:40 - 15:50 : Huygens for all Widefield to Super-resolution imagesExample data from customers of widefield to super-resolution microscopes, including STED, Airyscan and SMLM.
15:50 - 16:15 : Get started with HuygensOpening and converting images in Huygens, microscopy parameters and metadata handling, clipping, undersampling, viewing and inspecting the image, identifying possible issues.
16:15 - 16:25 : Break
16:25 - 16:35 : Deconvolution Express and Batch FeederFully automated deconvolution and batch processing on the fly
16:35 - 17:15 : Deconvolution Wizard and Twin SlicerUse the Deconvolution Wizard to obtain optimal results, inspect and compare image using the Twin Slicer.
17:15 - 17:40 : Batch processing with the Workflow ProcessorHow to design and schedule multiple deconvolution and restoration tasks with the Workflow Processor.
17:40 - 17:50 : Huygens Everywhere, remote display, web-based with multiple peopleWhether you are a single user or with many, a beginner or an expert, at home or in the office: Huygens offers a solution for all situations!
17:50 - 18:05 : Break
18:05 - 18:20 : True deconvolution and Reliable quantification of image dataOptimal Deconvolution settings, image/signal quantification, reproducible results.
18:20 - 18:40 : Super-resolution images and Huygens, incl PSF distillationHow to treat super-resolution images, and how to distill an experimental PSF from bead images, Microscope Quality Control, Compare experimental and theoretical PSFs easily
18:45 - 18:55 : Discussion - questions
18:55 - 19:00 Closing remarks - End of Day 1
Day 2 (Wednesday): Restoration, visualization and analysis
14.50 - 15:00 AM: Welcome - start videocallSee here how your day and time corresponds to 15:00 CEST.
15:00 - 15:05 : Recap day 1
15:05 - 15:20 : Localizer - SMLM and Cluster AnalysisAnalysis of 2D/3D single molecule localization microscopy data. Easy, Fast, and accurate with Huygens Localizer.
15:20 - 15:40 : Light Sheet Deconvolution & FusionFuse and deconvolve all your multiview Light Sheet data with Huygens Fuser.
15:40 - 16:00 : Tile StitchingStitching, automated/manual vignetting correction, and deconvolution combined in one Wizard.
16:00 - 16:10 : Break
16:10 - 16:45 : Colocalization AnalyzerMeasure colocalization coefficients and visualize them using Huygens' unique 3D colocalization map.
16:45 - 16:55 : Acquisition issues and how to correct themHow to deal with image distortions and acquisition pitfalls. Addressed issues include noise, blurring, bleaching, hotpixels, spherical aberration, bleedthrough, drift.
16:55 - 17:15 : Chromatic aberration correctionChromatic and spherical aberration have a significant impact on image quality and analysis. In this session you will learn how to identify and correct these issues in Huygens.
17:15 - 17:25 : CrosstalkCrosstalk can be disastrous for image visualization and analysis. Huygens helps you with detect and correct crosstalk.
17:25 - 17:40 : Object/image stabilizationMeasure and correct drift and rotation in space and time with Huygens Object Stabilizer.
17:40 - 17:55 : Break
17:55 - 18:20 : Object AnalyzerSegment and analyze objects within your image using Huygens Object Analyzer.
18:20 - 18:30 : Object TrackingAutomated object recognition & tracking with machine based learning and track analysis using Huygens Object Tracker.
18:30 - 18:50 : Quiz - Apply what you learnedUse Huygens optimally to identify and correct imaging issues in a demo image.