Virtual SVI Huygens Workshop

July 13th and 14th, 2021 (CEST, UTC +2)

This Workshop was held and recorded. A next live workshop is planned for Spring 2022

Get the best out of your microscopy image

With the SVI Huygens software you can unlock the true potential of your microscopy images. During this virtual workshop, you will learn how to acquire the best possible image and how to use the specialized Huygens features for restoring, visualizing and analyzing your microscopy data. Using a collection of demo images, you will get hands-on experience with our world-renowned software. Similar courses, previously held at the SVI headquarters, were rated very good to excellent by the participants.


Program interactive workshop July 2021

Amsterdam: Tuesday (13th) and Wednesday (14th) 5:20 - 9:30 CEST
Sydney: Tuesday (13th) and Wednesday (14th) 13:20 -17:30 AEST
Tokyo: Tuesday (13th) and Wednesday (14th) 12:20 -16:30 JST
Shanghai: Tuesday(13th) and Wednesday (14th) 11:20 -15:30 CST
Los Angeles: Monday (12th) and Tuesday (13th) 20:20 - 00:30 PDT

Day 1 (Tuesday): Deconvolution, data handling and quantification

All times are displayed in Central European Summer Time (CEST, UTC +2).

05.20 - 5:30 AM : Welcome

- See here how your day and time corresponds to 5:20 CEST.

05:30 - 6:00 : Introduction by Hans van der Voort (founder of SVI)

General introduction to microscopy image Formation, Deconvolution and Restoration
  • Light/Wave properties, Spatial frequencies
  • Deconvolution algorithms
  • Point spread function - Optical Transfer Function
  • Super-resolution imaging

06:00 - 06:10 : Deconvolution - Visualization - Analysis : A piece of cake

Demo of Huygens with an example image.

06:10 - 06:20 : Huygens supports images from Widefield to Super-resolution

Example datasets from widefield to super-resolution data of STED, Airyscan and SMLM.

06:20 - 06:45 : Get started with Huygens

Opening and converting images in Huygens, microscopy parameters and metadata handling, clipping, undersampling, viewing and inspecting the image, identifying possible issues.

06:45 - 07:00 : Break

07:00 - 07:40 : Deconvolution Wizard and Twin Slicer

Use the Deconvolution Wizard to obtain optimal results, inspect and compare image using the Twin Slicer.

07:40 - 08:00 : Batch processing with the Workflow Processor

How to design and schedule multiple deconvolution tasks with the Workflow Processor.

08:00 - 08:15: Huygens Everywhere, remote display, web-based with multiple people

Whether you are a single user or with many, a beginner or an expert, at home or in the office: Huygens offers a solution for all situations!

08:15 - 08:30 : Break

08:30 - 08:55 : Deconvolution and Reliable quantification of image data

Optimal Deconvolution settings, image/signal quantification, reproducible results.

08:55 - 09:20 : Huygens automated PSF generation and PSF distillation

How to distill an experimental PSF from bead images, Microscope Quality Control, Compare experimental and theoretical PSFs easily

09:20 - 09:30 : Discussion - questions

Day 2 (Wednesday): Restoration, visualization and analysis

05:20 - 05:30 AM: Welcome - start videocall

See here how your day and time corresponds to 5:20 CEST.

05:30 - 05:35 : Recap day 1

05:35 - 05:50 : Localizer - SMLM imaging

Analysis of 2D/3D single molecule localization microscopy data. Fast, accurate and easy with Huygens Localizer.

05:50 - 06:10 : Light Sheet Deconvolution & Fusion

Fuse and deconvolve all your light Sheet data with Huygens Fuser.

06:10 - 06:30 : Tile Stitching

Stitching, automated/manual vignetting correction, and deconvolution combined in one Wizard.

06:30 - 06:45 : Break

06:45 - 07:15 : Colocalization Analyzer

Measure colocalization coefficients and visualize them using Huygens' unique 3D colocalization map.

07:15 - 07:25 : Acquisition issues and how to correct them

How to deal with image distortions and acquisition pitfalls. Addressed issues include noise, blurring, bleaching, hotpixels, spherical aberration, bleedthrough, drift.

07:25 - 07:40 : Chromatic aberration correction

Chromatic and spherical aberration have a significant impact on image quality and analysis. In this session you will learn how to identify and correct these issues in Huygens.

07:40 - 07:55 : Crosstalk

Crosstalk can be disastrous for image visualization and analysis. Huygens helps you with detect and correct crosstalk.

07:55 - 08:15 : Object/image stabilization

Measure and correct drift and rotation in space and time with Huygens Object Stabilizer.

08:15 - 08:30 : Break

08:30 - 08:50 : Object Analyzer

Segment and analyze objects within your image using Huygens Object Analyzer.

08:50 - 9:00 : Object Tracking

Automated object recognition & tracking with machine based learning and track analysis using Huygens Object Tracker.

09:00 - 09:20 : Quiz - Apply what you learned

Get the best out of a microscopy dataset. Use Huygens optimally to identify and correct imaging issues in a demo image.

09:20 - 09:30 : Q&A, closing remarks and farewell