Virtual SVI Huygens Imaging Workshop
April 20th and 21st, 2022 (EDT/PDT/CEST, New York/Los Angeles/Amsterdam)
Get the best out of your microscopy image
Unlock the true potential of your microscopy images with the Huygens Software. During this free virtual workshop, you will learn how to acquire the best possible image and how to use the specialized Huygens features for restoring, visualizing and analyzing your microscopy data. Using a collection of demo images, you will get hands-on experience with our world-renowned software. Workshop material (incl. a manual, demoimages, and info to obtain the software) will be made available on time. Our two virtual courses in 2021 had more than 1K participants.Click here to see some reviews.
Program interactive workshop
Date: Wednesday (20th) and Thursday (21st), Time:
New York: 11:00 - 15:00 EDT
Los Angeles: 8:00 - 12:00 PDT
Anchorage (Alaska): 7:00 - 11:00 AKDT
Rio de Janeiro: 12:00 - 16:00 BRT
London: 16:00pm - 20:00 BST
Amsterdam: 17:00 - 21:00 CEST
Bucharest: 18:00 - 22:00 EEST
See here how your day and time corresponds to 11:00 EDT.
Day 1 (Wednesday): Deconvolution, data handling and quantification
All times are displayed in Eastern Daylight Time (EDT, New York).
10.50 - 11:00 AM : Welcome
11:00 - 11:30 : Introduction by Hans van der Voort (founder of SVI)General introduction to microscopy image Formation, Deconvolution and Restoration
- Light/Wave properties, Spatial frequencies
- Deconvolution algorithms
- Point spread function - Optical Transfer Function
- Super-resolution imaging
11:30 - 11:40 : Deconvolution - Visualization - Analysis : A piece of cakeDemo of Huygens with an example image.
11:40 - 11:50 : Huygens for all Widefield to Super-resolution imagesExample datasets from widefield to super-resolution data of STED, Airyscan and SMLM.
11:50 - 12:15 : Get started with HuygensOpening and converting images in Huygens, microscopy parameters and metadata handling, clipping, undersampling, viewing and inspecting the image, identifying possible issues.
12:15 - 12:30 : Break
12:30 - 12:40 : Deconvolution Express and Batch FeederFully automated deconvolution and batch processing on the fly
12:40 - 13:25 : Deconvolution Wizard and Twin SlicerUse the Deconvolution Wizard to obtain optimal results, inspect and compare image using the Twin Slicer.
13:25 - 13:45 : Batch processing with the Workflow ProcessorHow to design and schedule multiple deconvolution and restoration tasks with the Workflow Processor.
13:45 - 14:00 : Huygens Everywhere, remote display, web-based with multiple peopleWhether you are a single user or with many, a beginner or an expert, at home or in the office: Huygens offers a solution for all situations!
14:00 - 14:10 : Break
14:10 - 14:30 : True deconvolution and Reliable quantification of image dataOptimal Deconvolution settings, image/signal quantification, reproducible results.
14:30 - 14:50 : Huygens automated PSF generation and PSF distillationHow to distill an experimental PSF from bead images, Microscope Quality Control, Compare experimental and theoretical PSFs easily
14:50 - 15:00 : Discussion - questions
Day 2 (Thursday): Restoration, visualization and analysis
10.50 - 11:00 AM: Welcome - start videocallSee here how your day and time corresponds to 5:20 CEST.
11:00 - 11:05 : Recap day 1
11:05 - 11:20 : Localizer - SMLM imagingAnalysis of 2D/3D single molecule localization microscopy data. Fast, accurate and easy with Huygens Localizer.
11:20 - 11:40 : Light Sheet Deconvolution & FusionFuse and deconvolve all your multiview Light Sheet data with Huygens Fuser.
11:40 - 12:00 : Tile StitchingStitching, automated/manual vignetting correction, and deconvolution combined in one Wizard.
12:00 - 12:15 : Break
12:15 - 12:45 : Colocalization AnalyzerMeasure colocalization coefficients and visualize them using Huygens' unique 3D colocalization map.
12:45 - 12:55 : Acquisition issues and how to correct themHow to deal with image distortions and acquisition pitfalls. Addressed issues include noise, blurring, bleaching, hotpixels, spherical aberration, bleedthrough, drift.
12:55 - 13:15 : Chromatic aberration correctionChromatic and spherical aberration have a significant impact on image quality and analysis. In this session you will learn how to identify and correct these issues in Huygens.
13:15 - 13:25 : CrosstalkCrosstalk can be disastrous for image visualization and analysis. Huygens helps you with detect and correct crosstalk.
13:25 - 13:40 : Object/image stabilizationMeasure and correct drift and rotation in space and time with Huygens Object Stabilizer.
13:40 - 13:55 : Break
13:55 - 14:20 : Object AnalyzerSegment and analyze objects within your image using Huygens Object Analyzer.
14:20 - 14:30 : Object TrackingAutomated object recognition & tracking with machine based learning and track analysis using Huygens Object Tracker.
14:30 - 14:50 : Quiz - Apply what you learnedGet the best out of a microscopy dataset. Use Huygens optimally to identify and correct imaging issues in a demo image.