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Banner Workshop Oct2024 1

Free Virtual Workshop on "Object Analysis", October 24th, 2024

Includes workshop material and Huygens Everywhere trial version.


This 3 hour hands-on Workshop will be offered twice on the same day.


First Session: 9:30-12:30 CEST Amsterdam

10:30 - 13:30 EEST Athens; 16:30 - 19:30 JST Tokyo; 18:30 - 21:30 AEDT Sydney


Second Session: 15:30-18:30 CEST Amsterdam

9:30 - 12:30 EDT New York; 6:30 - 9:30 PDT Los Angeles





A workshop for all researchers interested in image analysis!


During previous workshops, you may have learned what it takes to become an expert in acquiring and restoring images, and in making them suited for image analysis. In this workshop, we delve deeper into obtaining reliable quantitative information from your microscopy data using the Huygens Object Analyzer. You will learn how to segment objects in 3D, apply watershedding, use regions-of-interest, track objects over time, and obtain information about objects within minutes. This information can include object count, size, shape, surface and intensity. Additionally, you will see how to measure distances (e.g., nearest neighbor) and colocalization between objects, and how to perform batch-wise analysis.
A workshop manual, demo images, and a temporary Huygens Everywhere trial will be provided for free. Join us and register now!

Program for both 3h sessions of this hands-on workshop:


Note that the times below are in CEST and for the first session. The schedule for the second session is 6 hours later.

9:30 - 9:35 : Welcome (2nd similar session starts at 15:30 CEST)


9:35 - 9:50 : Huygens Object Analyzer Intro (segmentation, ROI, anchors, filtering, coloring objects according to their properties)


9:50 - 10:30 : Used case 1: Telomere counting within nuclei (incl. Child-Parent analysis, clustering, batch analysis)


10:30 - 10:40 : Break - Q&A


10:40 - 11:15 : Used case 2: RNA-FISH analysis (incl. colocalization, distance, count, volume, intensity)


11:15 - 11:25 : Ongoing developments in AI-based segmentation and importing label images from open-source (AI) segmentation software


11:25 - 11:30 : Break - Q&A


11:30 – 11:45 : Importance of image QC and restoration, and Huygens analysis options


11:45 - 12:20 : Used case 3: Tracking target-induced movement and Ca2+ spikes of white blood cells (incl. velocity, MSD, workflows)


12:20 - 12:30 : Discussion - Q&A