Free Virtual SVI Huygens Imaging Workshop

April 25th and 26th, 2023 (EDT/PDT/CST/CEST, New York/Los Angeles/Beijing/Amsterdam)

Stay true to your imaged object!

Restore your valuable microscopy images & visualize and analyze them in the most reliable manner with Huygens. This free virtual workshop consists of lectures with in-depth information on various imaging topics, and demo/handson sessions with the Huygens Software. You will learn how to acquire the best possible microscopy data and how to use the specialized Huygens features for restoring, visualizing and analyzing your images. More than 96.4% of the attendents of the previous workshop were (very) satisfied. Click here to see some reviews.

Banner Workshop April2023 1

Program interactive workshop

Date: Tuesday (25th) and Wednesday (26st), Time:
New York: 9:00 - 13:00 EDT
Los Angeles: 6:00 - 10:00 PDT
Rio de Janeiro: 10:00 - 14:00 BRT
London: 14:00pm - 18:00 BST
Amsterdam: 15:00 - 19:00 CEST
Bucharest: 16:00 - 20:00 EEST
Beijing: 21:00 - 1:00 CST
See here how your day and time corresponds to 15:00 CEST.

Day 1 (Tuesday): Deconvolution, data handling and quantification

All times are displayed in Central European Summer Time (CEST, Amsterdam).

14.50 - 15:00 AM : Welcome

15:00 - 15:30 : Presentations of scientists

Speaker 1. Daniela Malide, PhD, Staff scientist in the light microscopy core facility of the National Heart Lung and Blood Institute (NHLBI) of the NIH, Bethesda, USA. Title: "Real- world examples from a light microscopy core experience in using Huygens software".

Speaker 2. Claudio Retamal, PhD, Assistent Prof. at CEBICEM, Facultad de Medicina y Ciencia, Universidad San Sebastian, Santiago, Chile. Title: "Intracellular trafficking, unravelling crossroads between endocytic and exocyctic routes",

15:30 - 15:40 : Deconvolution - Visualization - Analysis : A piece of cake

Full-scale demo with an example image

15:40 - 15:50 : Huygens for all Widefield to Super-resolution images

Example data from customers, including widefield, confocal, STED, Airyscan, and SMLM.

15:50 - 16:10 : Get started with Huygens

Opening and converting images in Huygens, scaling clipping, undersampling, viewing and inspecting the image, identifying possible issues.

16:10 - 16:20 : Break

16:20 - 16:35 : Metadata (image parameters)

The importance of proper metadata reading/writing for all microscopic files

16:35 - 16:45 : Batch Feeder

Deconvolving images (newly) acquired images on-the-fly.

16:45 - 17:25 : Deconvolution Express/Wizard + Twin Slicer

Use the Deconvolution Wizard to obtain optimal results, inspect and compare image using the Twin Slicer.

17:25 - 17:55 : Batch processing with the Workflow Processor

How to design and schedule multiple deconvolution, restoration and analysis tasks with the Workflow Processor.

17:55 - 18:05 : Break

18:05 - 18:15 : Huygens Everywhere, remote display, web-based with multiple people

Whether you are a single user or with many, a beginner or an expert, at home or in the office: Huygens offers a solution for all situations!

18:15 - 18:30 : True deconvolution and Reliable quantification of image data

Optimal Deconvolution settings, image/signal quantification, reproducible results.

18:30 - 18:50 : Experimental PSF distillation & Quality Control

How to distill an experimental PSF from bead images, Microscope Quality Control, Compare experimental and theoretical PSFs easily

18:50 - 18:55 : Discussion - questions

18:55 - 19:00 Closing remarks - End of Day 1

Day 2 (Wednesday): Restoration, visualization and analysis

14.50 - 15:00 AM: Welcome - start videocall

See here how your day and time corresponds to 15:00 CEST.

15:00 - 15:20 : Localizer - SMLM and Cluster Analysis

Analysis of 2D/3D single molecule localization microscopy data. Easy, Fast, and accurate with Huygens Localizer.

15:20 - 15:35 : Tile Stitching

Stitching, automated/manual vignetting correction, and deconvolution combined in one Wizard.

15:35 - 15:45 : Acquisition issues and how to correct them

How to deal with image distortions and acquisition pitfalls. Addressed issues include noise, blurring, bleaching, hotpixels, spherical aberration, bleedthrough, drift.

15:45 - 15:55 : Crosstalk

Crosstalk can be disastrous for image visualization and analysis. Huygens helps you with detect and correct crosstalk.

15:55 - 16:15 : Chromatic aberration correction

Chromatic and spherical aberration have a significant impact on image quality and analysis. In this session you will learn how to identify and correct these issues in Huygens.

16:15 - 16:25 : Break

16:25 - 16:40 : Object/image stabilization

Measure and correct drift and rotation in space and time with Huygens Object Stabilizer.

16:40 - 17:05 : 3D visualization/rendering

Huygens MIP, Surface and SFP renderers visualizes objects and extremely high quality. This handson introduces you to these options.

17:05 - 17:35 : Object Analyzer

Segment and analyze objects within your (multichannel) image using Huygens Object Analyzer, and color objects based on some of their properties.

17:35 - 17:45 : Break

17:45 - 18:15 : Colocalization Analyzer

Measure colocalization coefficients and visualize them using Huygens' unique 3D colocalization map.

18:15 - 18:30 : Object Tracking

Automated object recognition & tracking with machine based learning and track analysis using Huygens Object Tracker.

18:30 - 18:50 : Quiz - Apply what you learned

Use Huygens optimally to identify and correct imaging issues in a demo image.

18:50 - 19:00 : Q&A, closing remarks and farewell