As in past years, we've received many high quality images and movies for the ''Huygens Image Contest", and our job to select the winners was a challenging one. We like to thank all the participants for submitting their images for this contest.
After careful consideration, we made the decision to award the first prize of the 2018 contest to Dr. Outi Paloheimo from the Tampere Imaging Facility, University of Tampere, Finland. Mrs. Outi Paloheimo submitted a Huygens deconvolved Zeiss LSM780 confocal image of high quality that is Christmas card ready material. The image, which is displayed below and will appear on our 2018 Christmas card, represents Human induced pluripotent stem cell derived cardiomyocytes (heart cells) showing filamentous actin and nuclei. We congratulate Outi with winning an ASUS Chromebook.
All other participants of the 2018 contest received a bluetooth speaker for listening to their favorite Christmas music.
An overview of all the winners of the Huygens Image Contest in recent years can be found below. A big thanks to the participants for making the Huygens Image Contest into a great success and we hope you will join the next years Huygens Image Contest. Images and movies for contests can be submitted all year through, see also this years contest.
1st Prize: Dr. Outi Paloheimo. Tampere Imaging Facility, University of Tampere, Finland. Special thanks to the Heart Group, Faculty of Medicine and Health Technology, University of Tampere for providing the cells.
2nd Prize: Dr. Howard Vindin, Cell Biology Group, The Woolcock Institute of Medical Research, The University of Sydney, Australia. Special thanks also to Sydney Microscopy and Microanalysis, ACMM, University of Sydney.
3th Prize: Dr. Grazvydas Lukinavicius, Department of Nanobiophotonics, Max Planck Institute for Biophysical Chemistry, Germany.
1st Prize Winner
Image from Outi Paloheimo
This Huygens deconvolved confocal image shows Human induced pluripotent stem cell derived cardiomyocytes (heart cells) with filamentous actin in red, and nuclei in gray. The image was acquired with a Zeiss LSM780 microscope with a 63x/1.40 oil immersion objective, 561 nm and 405 nm laser lines. Scaling X, Y 0.047 µm, scaling Z 0.150 µm (z range 8.40 µm). Image size 192x192 µm (xy). Deconvolution was performed with Huygens Essential (18.04) using a theoretical PSF and the CMLE algorithm.
2nd Prize Winner
Movie submitted by Howard Vindin
Autofluorescence of thick elastic fibers (red) and SHG signal (collagen, green) in part of the Pulmonary pleurae from a human lung. The image was acquired on a Leica SP8 two photon microscope (63x 1.4NA), deconvolved with Huygens Professional and visualized using the Huygens SFP renderer. Stack dimensions are 84x84x18 μm.
Image from Grazvydas Lukinavicius
Huygens deconvolved image of fruit fly tissue stained with Tubulin probe (magenta) and tracheole (yellow). This high resolution and quality image was acquired using a STED 775 QUAD scanning microscope (Abberior Instruments GmbH).
1st Prize: Dr. Dimitris Kapsokalyvas, Department of Molecular Cell Biology, Maastricht University,The Netherlands
3rd Prize: Dr. Tagide DeCarvalho, Department of Biological Sciencess, University of Maryland, Baltimore County, USA.
4th Prize: Dr. Gokhan Yilmaz, Department of Pharmacology, University of Oxford, UK.
5th Prize: Dr. Motosuke Tsutsumi, Nikon Imaging Center at Hokkaido University, Japan
1st Prize Winner
Image from Romain Guiet
Hela cell stained for DNA (Azure), F-Actin (Spring Green), Mitochondria (Amber) and Tubulin (Bright Pink). Confocal images were taken with a Zeiss LSM710 microscope and deconvolved with Huygens software.
1st Prize Winner
Image submitted by Dimitris Kapsokalyvas
SHG signal of the rat soleous muscle's myosin imaged with a Leica two-photon microscope (NA: 1, 20x). Stack has been deconvolved with Huygens using a measured PSF, and visualized with the Huygens SFP Renderer. High quality imaging together with Huygens allows you to see through the muscle's A bands from one side to the other. The stack dimensions is 105x105x87 μm.
Image in the category "Tissue and Organisms", from Tagide DeCarvalho
This image of a cyanobacteria mat/biofilm, which is stained with acridine orange, shows much color that is mostly due to autofluorescence. This aesthetic image was acquired on a Leica confocal, and deconvolved and MIP rendered with Huygens. It could well have been a product of modern art.
Image submitted by Gokhan Yilmaz
This colorful picture is a high-resolution image of Niemann-Pick Disease Type C (NPC) cells taken with a Leica STED using 100% 775 STED depletion. It shows after deconvolution with Huygens much detail and striking weak filopodia formation in the NPC cells.
Movie submitted by Motosuke Tsutsumi
This movie was made with Huygens and shows fixed HeLa cells stained with Hoechst 33342 and alpha-tubulin. The 3D image was acquired with a Nikon A1 confocal (60x, NA 1.4) and deconvolved using Huygens Essential Deconvolution Express.
1st Prize: Dr. Daniela Malide, Light Microscopy Core Facility of the NIH - National Heart, Blood and Lung Institute, Bethesda, USA.
1st Prize: Mrs. Outi Paloheimo from the Neuro Group BioMediTech, and Imaging Facility, University of Tampere, Finland.
3rd Prize: Dr. Priyam Banerjee, MD Anderson Cancer Center, Houston, Texas, USA.
4th Prize: Mrs. Julia Sauerwald, Group of Prof. Stefan Luschnig, Institute for Neurobiology, WWU Muenster, Germany.
5th Prize: Dr. Eva Wegel, JIC BioImaging, John Innes Centre, Norwich, United Kingdom.
1st Prize Winners
Image in the category "Cells", from Daniela Malide
COS-7 (fibroblast-like) cell line with mitochondria in green/white and cytoskeletal protein tubulin in red/magenta. Comparing the diffraction limited confocal image (green/red) with the super-resolution STED (Stimulated Emission Depletion) microscopy image (white/magenta). STED reveals thin tubulin strands (magenta) and details of the TOM-20 stained outer mitochondria membrane (white). Images were taken with a Leica SP8 STED 3X microscope and deconvolved with Huygens software.
Image in the category "Tissue and Organisms", from Outi Paloheimo
The image represents a portion of the wing (showing arrangement of scales) of ''Pieris brassicae'' (cabbage butterfly). A Zeiss LSM 780 confocal microscope (25x/0.8 objective) with excitation lasers 488 nm, 535 nm, 633 nm was used to image the autofluorescence. The image was deconvolved using Huygens Essential (CMLE and theoretical PSF).
Image submitted by Priyam Banerjee
Human lung adenocarcinoma tissue with the extracellular matrix protein Collagen I immunostained in red (Alexa Fluor 568), collagen modifying enzyme Lysine Hydroxylase II (LH2) immunostained in green (Alexa Fluor 488). Nuclei were counterstained in blue (DAPI). Image was acquired with a Nikon A1+ confocal using Plan Apo 10X 0.45 NA objective. The image was deconvolved and SFP rendered with Huygens Professional version 16.10.
Image submitted by Julia Sauerwald
The image shows a Drosophila thorax captured with a Zeiss 880 confocal microscope and deconvolved using Huygens Essential. Respiratory tracheal branches are labeled in various colors using stochastic expression of spaghetti monster GFP with different epitope tags.
Image submitted by Eva Wegel
The image is a maximum intensity projection (MIP) of a z-stack through a ''Drosophila'' macrophage imaged on a Leica TCS SP8X scanning confocal microscope. Objective NA 1.4. Pinhole size 405nm 0.17 AU, 488nm 0.15 AU, 568nm 0.15 AU for obtaining the best possible resolution.
Nuclei are DAPI stained (cyan), alpha-tubulin was detected with a primary mouse DM1A antibody and a secondary anti-mouse antibody coupled to Alexa Fluor 488 (magenta), actin was labelled with phalloidin coupled to Alexa Fluor 568 (green). Deconvolution was done with CMLE using Huygens Essential default settings.
1st Prize: Dr. Pawel Pasierbek, BioOptics Facility of the IMP-IMBA-GMI, Vienna, Austria
2nd Prize: Dr. Matyas Molnar, BioVis Facility, Uppsala University, Sweden
3rd Prize: Dr. Helfrid Hochegger, Genome and Stability Centre, University of Sussex, UK
4th Prize: Dr. Arndt Meyer, Animal Navigation Group, University of Oldenburg, Germany
5th Prize: Dr. Jorge Bernardino de la Serna, Science and Technology Facilities, Rutherford Appleton Laboratory, UK
6th Prize: Dr. Jing Yan, Department of Molecular Biology, Princeton University, USA
Image from Pawel Pasierbek, BioOptics (IMP, IMBA, GMI), Vienna, Austria.
Ant head autofluorescence captured with a Zeiss 710 confocal microscope using 488nm laser excitation and detection of two channes (blue and green). The acquired 3D dataset was deconvolved and maximum intensity projection image was rendered with Huygens.
Image submitted by Helfrid Hochegger, Genome and Stability Centre, Sussex Universty, UK.
The image shows glioma stem cells grown on matrigel and stained by IF
with tubulin (green, actin (red), centromeres/CREST (white) and DAPI (blue). The image was taken on an Olympus IX73 using a 40x 0.9NA.
Image submitted by Arndt Meyer, Animal Navigation Group, University of Oldenburg, Germany.
Calbindin immunoreactivity in the ganglion cell layer of a mouse retina. The image was taken close to the center of the retina, where the ganglion cell axons gather in thick bundles and then leave the eye as the optic nerve. This confocal stack was recorded on a Leica SP8 microscope, deconvolved using Huygens Essential, and finally color-coded for depth.
Image submitted by Jorge Bernardino Rutherford Appleton Laboratory, UK.
Image of a T lymphocyte crawling taken with a Leica SP8 g-STED.
The image is deconvolved with Huygens and the final visual effect was obtained using the Huygens surface rendering tool.
Blue shows labelled plasma membrane with a specific lipophilic dye, red represents the actin filaments, and yellow internal cytosolic vesicles.
Image submitted by Jing Yan, Princeton University, USA.
Image shows a growing community of bacteria Vibrio cholerae, the pathogen for cholerae. Bacteria adopt a social life form known as biofilm, where they stay together and secrete chemicals to build a "home" for survival. This Nikon spinning disc confocal image was deconvolved with a theoretical PSF in Huygens and finally color coded for depth.
Image from Imre Gaspar of the EMBL Heidelberg using the Leica SP8 and Huygens Remote Manager/Core at the Advanced Light Microscopy Facility, Germany.
The image represents a Z-stack of 116 sections showing a series of developing Drosophila oocytes with actin in yellow (Phalloidin) and the nuclear envelope of the nurse cells in green (GFP-Tm1).
Seema S. Lakdawala and Juraj Kabat of the National Institute of Allergy and Infectious Disease (NIAID), NIH, Bethesda, USA: This Leica SP5 confocal image was deconvolved with Huygens.
The image visualizes a MDCK cell that was infected with influenza A H1N1 virus and stained with fluorescent probes targeting 4 distinct influenza viral RNA segments, PB2 (red), PB1 (green), PA (orange), and NP (yellow). The nucleus is labeled in blue based on DAPI staining.
Leica SP8 confocal image from Yury Belyaev of the ALMF-EMBL Heidelberg (Germany). The image was found and selected using Huygens Titan, and deconvolved and visualized with Huygens Remote Manager and Core.
The image was acquired with a Leica SP8 confocal with a water objective (NA 1.1) and represents a fish eye with membrane in green and nuclei in red.
Image from Karin Panser from the laboratory of Dr. Andrew Straw, Institute of Molecular Pathology (I.M.P.), Vienna, Austria.
The image represents fruit fly (Drosophila melanogaster) ommatidia, which are arranged in an extremely regular array in the compound eye. Nuclei are shown in blue (DAPI), cadherin in red, and chaoptin in the photoreceptors in green. This Zeiss LSM780 confocal (NA 1.3, 40x) image was deconvolved with Huygens Professional.
Dr. Ulrike Engel, Nikon Imaging Center, BioQuant Institute, Heidelberg, Germany
Huygens deconvolved and MIP rendered confocal image (Nikon; NA 0.45) of a tadpole intestine with nuclei in orange and muscle actin in cyan. The direction of the gut looping can be used as a readout for proper asymmetric organ development. Looping in the other direction is a sign of perturbed Nodal/Notch signaling.
Matthew Mitschelen from the Reynolds Oklahoma Center on Aging, University of Oklahoma Health Sciences Center, USA
Huygens deconvolved and MIP rendered epifluorescence Z stack of part of a mouse brain. The image shows aquaporin 4 (red) as individual astrocytic end-feet along a blood vessel. Thanks to deconvolution, GFAP (green) can be colocalized with AQ4 in small areas, but the end-feet and arms of astrocytes can be identified as distinguishable structures in terms of protein expression.
Ms. Thuraya Awadová and Mr. Martin Šodek, Veterinary research institute, CEITEC, Czech Republic
Dr. Thomas Ruckelshausen and Dr. Henrike Peuschel, Leibniz Institute of New Materials, Germany
Dr. Claudia Florindo, Dr. Marco A. Campinho, and Javier Padilla, University of Algarve, Portugal