Scientific Volume Imaging
Movie Gallery
mesoSPIM image of a mouse brain
Visualized with the Huygens surface renderer and MIP. Courtesy of Nikita Vladimirov, Urs Ziegler, and Joana Martins from Center for Microscopy and Image Analysis, Universität Zurich, Switzerland.
Mitochondrial cristae dancing
Amazing STED time series of mitochondrial cristae. This STED dataset was deconvolved and visualized in Huygens SFP renderer. Image courtesy of Dr. Ana Andjelković and Dr. Rolando Berlinguer Palmini, The Bio-Imaging Unit, Newcastle University, United Kingdom.
Chicken embryo
Light sheet data from a Zeiss Z1 of a cleared chicken embryo at 4.5 days of development, rendered in the Huygens SFP renderer with an isosurface and moving cutting planes. Sample was cleared with 3DISCO technique and stained for neural crest, peripheral nervous system; dermomyotome, muscle progenitors and dorsal neural tube; and differentiated muscles. Courtesy: Prof. Christophe Marcelle, Mrs. Marie Julie Dejardin (INMG) & Dr. Denis Ressnikoff (CIQLE), Université Lyon 1, France.
Whole Organoid
A full organoid stained for the nucleus (braun) and mitochondria TOM20 (blue), was imaged and deconvolved and SFP rendered with Huygens Professional. Courtesy of Dr. Berlinguer Palmini Rolando, The Bio-Imaging Unit, Newcastle University, United Kingdom.
Rotation of a Paramecium made with the Huygens SFP renderer
This Leica confocal image was deconvolved and Surface Rendered with Huygens. Courtesy of A. Aubusson-Fleury CNRS, Gif sur Yvette, Paris.
Dendritic Spines
Detailed structure of dentate granule cell dendritic spines. Dr. Thomas Chater, Riken Brain Science Institute, Japan
3D confocal image and STED 3D dataset of Marine Dinoflagellate Amphidinium microtubular skeleton.
Both the confocal (red) and STED 3D channel (green) are deconvolved with the Huygens Professional Package and 3D rendered with the Huygens Surface Renderer. The movie was created with the Huygens Movie Maker. The novel Leica TCS SP8 STED 3X system now also allows for super-resolution in the axial (z) direction, while also keeping the conventional super-resolution in lateral (x,y) directions. The special Huygens STED 3D deconvolution module provides significant noise reduction, contrast increase and even more resolution increase for these powerful STED systems. Specifically notice that in confocal mode (red) the structure is completely blurred out in axial direction due to the confocal diffraction limit, while the STED 3X deconvolved image demonstrates that the fine microtubular structure can be easily resolved thanks to the additional super-resolution in axial direction.
Original dataset, both confocal and STED 3X mode are imaged with a Leica TCS SP8 STED 3X system.
Microtubular staining after methanol fixation using a primary antibody against a Tubulin and an Oregon Green 488 conjugated secondary antibody. Acknowledgement: Elisa Berdalet, CSIC Institute of Marine Sciences/Timo Zimmermann, Center for Genomic Regulation, Barcelona
Tubulin with STED
3D STED images of tubulin labeled with a small molecule probe, and imaged on an Abberior STED expert line. Deconvolved and visualized with the MIP renderer. Images kindly provided by Dr. Grazvydas Lukinavicius, MPI for Biophysical Chemistry - Department of NanoBiophotonics, Max Planck Institute, Germany.
Jurkat T-cell making contact with a Raji B-cell, stained for Raji cytosol (FURA-2, blue), T-cell receptor (anti-CD3 Alexa647, green), and Actin (Lifeact-mRFPruby, red).
Animated SFP rendering of a deconvolved time series. Original data was imaged using a Zeiss Cell Observer HS system with a 40x Fluor 1.3NA oil lens and deconvolved using Huygens Essential. The original time interval is 1 minute. "We look at the molecular events and redistribution of certain markers for T-cell activation like the TCR (green) and Actin (red). In the time series a ring-like pattern of Actin and a bulls-eye pattern of CD3 can be seen, accumulating at the contact-site (the immunolgical synapse. Both structures gave us a hint that the T-cell was activated properly." Recorded by Christian Junker M.Sc., Institute of Biophysics, University of Saarland, Germany.
SFP Rendering of a cell cluster.
Imaged using an inverted Leica TCS SP2 AOBS confocal microscope and deconvolved using Huygens Professional. The original data was recorded by Dr. Nicolas Fete, Laboratory of Stem Cells Dynamics, Swiss Federal Institute of Technology of Lausanne, Switzerland.