Images processed with Huygens in the field of Neurosciences

This is an image of neurons growing in the tissue culture, acquired with spinning disk confocal and deconvloved with Huygens professional.

Dr. Pawel Pasierbek, Institute of Molecular Pathology, BioOptics (IMP, IMBA, GMI), Vienna, Austria.

This image captures two fluorescently-labelled neuronal proteins (red and green) differentially expressed in the two neurons making ‘contacts’ to communicate with each other at the neurites (arms of the neuron). The image is a maximum projection of Z-stack captured on the Zeiss AxioImager, z-stacks were deconvolved with Huygens Professional.

Dr. Annie Quan, Children's Medical Research Institute (CMRI), Sydney University, Australia.

A mouse neuron primary cultured cell (fixed), immunostained by mouse alpha-tubulin antibody conjugated with Alexa 568 dye. For two-color image, the cell was also stained by Alexa488-phalloidin (F-actin would be stained). Microscopy: Nikon A1 confocal. Object: 20x Plan Apo VC (NA 0.75) dry. Deconvolution: by using Huygens Essential software, Deconvolution wizard (SN ratio was optimized for each image).

Dr. Motosuke Tsutsumi, Nikon Imaging Center, Hokkaido University, Japan.

Primary hippocampal neuronal culture from P1 C56Bl/6H mice, grown on glass bottom dishes, imaged live in aqueous medium, expressing CMV-driven mCherry (red) and mouse diacylglycerol lipase (with pAcGFP, green) Images were recorded with 1,4 NA 60x oil immersion objective on a Nikon Ti-E inverted confocal microscope.

Dr. Barna Dudok MSc., Hungarian Academy of Sciences, Hungary.

Huygens stitched tiles of widefield (Leica) fluorescent Leica LIF data. The data is shown with Huygens automatic vignetting and shading correction. Image represents a developing mouse cortex (P30) stained for Tbr1 and reelin.

Dr. Kolk and Dr. Witteveen, Nijmegen Centre for Molecular Life Science, The Netherlands.

Inhibitory terminals on spinal cord neuron. Photomicrograph showing a pattern of Glycine receptor (red), Gephyrin (blue) and GABA(A) receptor (green) immunolabeling in lumbar L3 segment of the rat spinal cord. The sections were examined with confocal microscopy, Zeiss 780, objective magn. x 40. Image was deconvolved with Huygens Professional software.

Ms. Anna Maciejewska, Laboratory of Reinnervation Processes, Nencki Institute of Experimental Biology, Poland.

Image of DAPI (Red) and mab22C10 (Green) staining in larval brain of Drosophila melanogaster. The image was captured using Leica SP5II confocal microscope.

Dr. Anand Krishna Tiwari, Indian Institute of Advanced Studies, India.

The image shows a Drosophila thorax captured with a Zeiss 880 confocal microscope and deconvolved using Huygens Essential. Respiratory tracheal branches are labeled in various colors using stochastic expression of spaghetti monster GFP with defferent epitope tags.

Prof. Stefan Luschnig, WWU Muenster, Institute for Neurobiology, Germany.

SuperResolution Images of Actin Cytoskeleton of NPC Disease. Image is taken with Leica STED Microscope with %100 775 STED depletion laser power and shows the reduced binding of the IgG-opsonized beads on actin cytoskeleton of NPC macrophages (stained with Aberrior 635 - Phalloidin).

Mr. Gokhan Yilmaz, Pharmacology, University of Oxford, United Kingdom.

Image of multi-focal amyloid plaque in the brain of a prion-infected mouse. The plaque was visualized by staining with the amyloid-binding fluorescent dye Thioflavin S. Image acquired by widefield epi-fluorescence microscopy, deconvolved with Huygens.

Dr. Gerald Baron, DIR - Laboratory of Persistent Viral Diseases, NIAID-NIH, United States.

Huygens deconvolved and MIP rendered epifluorescence Z stack of part of a mouse brain. The image shows aquaporin 4 (red) as individual astrocytic end-feet along a blood vessel. Thanks to deconvolution, GFAP (green) can be colocalized with AQ4 in small areas, but the end-feet and arms of astrocytes can be identified as distinguishable structures in terms of protein expression.

Matthew Mitschelen, Department of Geriatric Medicine, The University of Oklahoma Health Sciences Center, United States.

Image of Drosophila brain taken with Zeiss Z1 Light Sheet microscope. The images was deconvolved and fused with the Huygens Software.

Dr. Denis Ressnikoff, University Claude Bernard, Lyon, France.