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Olympus Fluoview 500


This is part of the Backprojected Pinhole Calculator.

Pinhole reported as a side length in microns


Details


From Olympus R&D: the microscope reports the side-length of a square pinhole in microns (µm). Use this reported value (named C.A, part of the general information in the metadata-file, right below Confocal 'ON") as an input in the form below.

The shape factor is c=1/\sqrt{\pi}\approx 0.5642.

The system magnification is 3.796, as reported by Olympus engineers but the value that you are asked to fill in below is the "Objective Magnification" (usually 60X or 100X).


Calculator

Pinhole side (microns)
Objective magnification