Plant Biology

Images processed with Huygens in the field of Plant Biology

Tobacco Epidermal Cells. The images were acquired with a Leica SP8, using the objective 20X water-immersion. Leaves of tobacco were infiltrated with a construct expressing a fluorescent GFP protein that goes into the endoplasmic reticulum of plant cells (green channel), while chloroplasts were imaged thanks to the autofluorescence in the red channel.

Dr. Paolo Margaria, Leibniz-Institut DSMZ, Braunschweig, Germany.

This image of transgenic MAP4-GFP Arabidopsis thaliana leaf was acquired during our study of cytoskeleton drugs effect on cytoskeleton-chloroplast interactions and leaf lipidome. Microtubules are shown in cyan, chloroplasts revealed by their autofluorescence in magenta-red. Images was taken with Carl Zeiss LSM 710 confocal microscope, and deconvolved using the Huygens Professional software.

Gregory Pozhvanov Ph.D, St. Petersburg State University, Russia.

A confocal autofluorescence image of mixed pollen deconvolved and rendered in Huygens. Autofluorescence was captured as three emission wavelengths using a Leica SP8 confocal. Z stack deconvolved using theoretical PSF then rendered as a MIP in Huygens Essential.

Dr. Glyn Nelson, Institute for Ageing and Health, University of Newcastle upon Tyne, United Kingdom.

Autofluorescence from pollen grains was captured with Zeiss 710 NLO multiphoton microscope in three channels using 850nm laser excitation. Two channels (blue and magenta) show two-photon autofluorescence in different spectral ranges, the third channel (green) shows second harmonic generation from starch particles inside the pollen grain. The acquired 3D dataset was deconvolved and maximum intensity projection image was rendered with Huygens.

Dr. Matyas Molnar, BioVis imaging facility, Uppsala University, Sweden.

The image shows tubulin in the Arabidopsis plant leaf deconvolved and fused with Huygens. A detail of the stomata and a line profile through these structures show a significant boost in resolution and signal to noise ratio. Two images were acquired separately with illumination from opposing sides with the Zeiss Z1

Dr. Shingo Nagawa, Cell Biology Core Facility, Shanghai Center for Plant Stress Biology, CAS, China.

Sample of spores and hyphae from the mushroom Psilocybe cyanescens. Taken on an SP5 Leica confocal laser scanning microscope and restored with Huygens.

Tagide deCarvalho PhD, University of Maryland Baltimore County, United States.

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