Zeiss LSM410 inverted
This is part of the Backprojected Pinhole Calculator.
Pinhole reported as P_8 parameter
Details
The pinhole size is reported by the microscope as an 8-bit number which linearly maps to the side length of the square physical pinhole as in the following table:
| Reported parameter (P_8) | Side length (µm) |
|---|---|
| 0 | 0 |
| 255 | 1000 |
Use the reported value as an input directly in the form below, it will be translated automatically accordingly to that table.
The shape factor is $$c=1/\sqrt{\pi}\approx 0.5652$$.
The system magnification is reported to be 2.23.
Calculator