Zeiss LSM410 inverted

This is part of the Backprojected Pinhole Calculator.

Pinhole reported as P_8 parameter

Details

The pinhole size is reported by the microscope as an 8-bit number which linearly maps to the side length of the square physical pinhole as in the following table:

Reported parameter (P_8) Side length (µm)
0 0
255 1000

Use the reported value as an input directly in the form below, it will be translated automatically accordingly to that table.

The shape factor is $$c=1/\sqrt{\pi}\approx 0.5652$$.

The system magnification is reported to be 2.23.

Calculator

 Reported parameter (P_8) Objective magnification