Zeiss LSM510

This is part of the Backprojected Pinhole Calculator.


The size of the circular pinhole is given by the microscope as a diameter d expressed in microns (µm). Use this value in the form below.

The system magnification is reported to be 3.33. The simplified equation to calculate the Back Projected Pinhole Radius in nanometers is therefore r_b\approx 500 d / (3.33 m_{obj}).


Pinhole diameter (microns)
Objective magnification

Huygens ×
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Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands

Phone: +31 (0)35 64216 26
Fax: +31 (0)35 683 7971
E-mail: info at svi.nl

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