Zeiss LSM510

This is part of the Backprojected Pinhole Calculator.


The size of the circular pinhole is given by the microscope as a diameter d expressed in microns (µm). Use this value in the form below.

The system magnification is reported to be 3.33. The simplified equation to calculate the Back Projected Pinhole Radius in nanometers is therefore $$r_b\approx 500 d / (3.33 m_{obj})$$.


Pinhole diameter (microns)
Objective magnification