Zeiss LSM710

This is part of the Backprojected Pinhole Calculator.

Details

The microscope reports the side length (s) of a square pinhole in microns (µm). Use this reported value in the form below.

Because of the square pinhole, the shape factor is: $$c=1/\sqrt{\pi}\approx0.5642$$ The system magnification is reported to be 1.9048. The simplified equation to calculate the Back Projected Pinhole Radius in nanometers is therefore: $$r_b\approx 564 s / (1.9048 m_{obj})$$

Calculator

Pinhole side (microns)
Objective magnification