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Microbiology & Molecular Biology

Images processed with Huygens in the field of Microbiology & Molecular Biology


Image of cyanobacteria mat/biofilm. Taken on an SP5 Leica confocal laser scanning microscope and restored with Huygens. Specimens are stained with acridine orange, but much of the color is due to autofluorescence.

Tagide deCarvalho PhD, University of Maryland Baltimore County, United States.

Lyngba cyanobacteria. Taken on an SP5 Leica confocal laser scanning microscope and restored with Huygens. Specimens are stained with acridine orange, but much of the color is due to autofluorescence.

Tagide deCarvalho PhD, University of Maryland Baltimore County, United States.

Release of GFP-containing viral particles from the infected cell. Z-stack was acquired on a Zeiss LSM700 confocal microscope with a 63X 1.4NA PlanApochromat oil objective and deconvolved with Huygens Professional using measured PSF. 3D reconstruction was performed in Surface Render of Huygens Professional. The image color was set using the watershed object segmentation and the full color range.

Dr. Elena Frolova, Department of Microbiology, University of Alabama at Birmingham, United States.

Image shows a growing community of bacteria Vibrio cholerae, the pathogen for cholerae. Bacteria adopt a social life form known as biofilm, where they stay together and secrete chemicals to build a "home" for survival. This Nikon spinning disc confocal image was deconvolved with a theoretical PSF in Huygens and finally color coded for depth.

Dr. Jing Yan, Princeton University, United States.


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