Loading...
 

Huygens Crosstalk Corrector

Learn & Support


Manual

User Guide

Detailed information on the Huygens Crosstalk Corrector.


Download User Guide

Huygens Imaging Academy

Huygens Imaging Academy

Learn all about getting the best images and the most reliable results for your publications.


View Imaging Academy >

Webinar

Learn more about the Huygens Crosstalk Corrector via the Webinar.


View more Webinars >


FAQ


Crosstalk (aka bleed-through or crossover) is a serious problem when analysing multichannel microscopy images, because you record some signal as a certain dye when it really comes from a different one. With Huygens CrossTalk Corrector you can quickly estimate and correct crosstalk between multiple channels simultaneaously. Learn more
In fluorescence microscopes, crosstalk (also frequently referred to as bleedthrough) can occur when acquiring a multi-channel image. In that case, the emission radiation of a given emission wavelength is detected by the wrong detector because part of the photons go through the wrong optical path inside the microscope (e.g. because the filters efficiency is not 100%). Therefore, some signal is actually recorded as coming from a certain dye when it really comes from a different one.
There are three ways to set the crosstalk between channels. The simplest is to press the ESTIMATE button and let the tool determine the crosstalk coefficients. If the estimation is not satisfactory the crosstalk coefficients can be adjusted manually. The crosstalk coefficients can be manually adjusted by moving the handles within the 2D histogram, or by switching to matrix view and changing the elements in the matrix


Contact & Help Center


Did not find the answer you were looking for? Please feel free to contact us or go to our help center to find more information.


Help Center Contact