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Huygens Crosstalk Corrector

Estimate and correct crosstalk for reliable analysis.



Crosstalk (aka bleed-through or crossover) is a serious problem when analysing multichannel microscopy images, because you record some signal as a certain dye when it really comes from a different one. With Huygens CrossTalk Corrector you can quickly estimate and correct crosstalk between multiple channels simultaneaously. Consider also Chromatic Aberration Corrector if you continue with colocalization or object-based analysis.

Image description:
Mitochondrial (TMRM) red signal is also detected as signal in the green Lipid Droplet (BODIPY) channel due to crosstalk (left side of the image). This crosstalk will have a serious impact on the analysis of lipid droplet structure. The right side of this image was corrected with Huygens Crosstalk Corrector. This spinning disk image was kindly provided by Kevin Knoops (Microscopy CORE Lab), Sabine Daemen and Matthijs Hesselink, Maastricht University, The Netherlands.

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Automatic and Easy

Quickly access if your image suffers from crosstalk and correct with just two button clicks.

Accurate correction

2D histograms show the estimated and corrected crosstalk with a MIP of the corrected image.

Coefficient Matrix

Use the coefficient matrix to verify and change the crosstalk coefficients of all channels (up to 32).


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All show Crosstalk
All Crosstalk corrected
This two channel multiphoton image shows a live mouse brain (anesthetized animal), expressing GFP in microglia cells and CFP in astrocytes. Because the emission spectra overlap and NDD detectors were used, there is a very serious problem with crosstalk/bleedthrough falsely suggesting there are double stained cells (see arrowheads). Huygens Crosstalk Corrector succesfully corrects the bleedthrough. Image kindly provided by Dr. Dimitra Thomaidou group, Neurobiology department and Light Microscopy Unit, Hellenic Pasteur Institute, Greece.

See more: Images in the field of NeuroBiology


Available for Huygens Essential & Professional

The Huygens Crosstalk Corrector option can be tested with a free test license. The product is commercially available as a licensed option for Huygens Essential and Professional.

Why wait? Try out this option by downloading Huygens and request a test license, or receive pricing information.

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