Scientific Volume Imaging

Yes. Huygens treats the image as the only known plane of a 3D stack and proceeds as usual. Before deconvolving, go to the Microscopic Parameters and set the z-sampling distance to the Nyquist rate as explained in 'sampling densities' (Huygens User Guide). For optimal results we do advise to image in 3D when possible, especially when performing quantitative analysis after deconvolution.