How to cite the use of Huygens in scientific papers.
Good scientific practice demands a complete description of used materials and methods. If you plan to publish a scientific paper or book (chapter) for which Huygens was used, you should describe how microscopy images were acquired and processed. You can adjust the following example sentence to refer to the use of Huygens for your article:All images were deconvolved with Huygens Professional version [version number] (Scientific Volume Imaging, The Netherlands, http://svi.nl).
Huygens product name (Essential, Professional, Localizer, HRM/Core) and version number should be adjusted to the software you've used, and can be found at the top of Huygens main window. More detailed information is shown in the Help->About menu, and is saved within the log file of each task performed by the Workflow Processor.
To allow the scientific community to repeat your experiments accurately, it is advised to also include settings of the most important deconvolution parameters (used algorithm; Signal to Noise ratio; number of iterations). Some examples:
All images were deconvolved with Huygens Essential version 22.10 (Scientific Volume Imaging, The Netherlands, http://svi.nl), using the CMLE algorithm, with Acuity: -5.3 (present since version 22.04), SNR:20, 40 iterations, and background value: 34.334. Or all images were deconvolved using Huygens Essential 22.10 with "Standard" as the Express Profile, and acuity: -5.3.
Huygens Software 22.10 was for all images to correct for crosstalk using the estimated values, and to perform deconvolution using the "Standard" Deconvolution Express Profile with Acuity set to: -5.3 (Scientific Volume Imaging, The Netherlands).
When using an experimental PSF for deconvolution, it is advised to make the file available. Also, any additional restoration steps applied before or after deconvolution should be described in detail and settings can be provided as a template file).
Once your paper has been accepted, we would be very happy to post a link to your publication on our website. You can submit a reference to your paper via this Submit References form.