The Huygens Software offers a wide range of visualization tools with which microscope images can be beautifully visualized. SVI has a long experience in visualization next to its strong focus on deconvolution. Several interactive 3D visualization tools are standard included in the Huygens Essential and Professional. These tools can be extended with additional visualization options.The visualization tools support GPU acceleration combined with CPU performance, which greatly reduces render times. Rendered results are identical between CPU and GPU, and thus enables dynamic switching.

Image Description:
Erythrocytes that mark the developing vasculature in a mouse embryo (E14.5). This 8x11x2mm specimen was imaged on a multiphoton microscope excited at 790nm using a 25x 1.0NA Objective. Subsequently, the image was stitched, deconvolved with Huygens Professional using a measured PSF, and visualised with depth-coded colouring using the Huygens MIP renderer. Image Imaged by Howard Vindin (Weiss Laboratory, School of Life and Environmental Sciences, Faculty of Science, University of Sydney)

The Huygens Surface Renderer is a powerful 3D visualization tool that enables the visualization of isosurfaces of volumes.

Image Description:
The Huygens Surface renderer window showing a Widefield image of a kinetochore complex. Data courtesy of Dr. Livio Kleij, Medical Oncology, UMC Utrecht, The Netherlands.

Surface Renderer Request Trail

The Movie Maker is a tool that allows the user to easily create sophisticated animations of multi-channel 3D images using the powerful Huygens visualization tools.

Image Description:
An overview of the Movie Maker interface, showing the storyboard, the preview and the timeline.

Movie Maker Request Trail

Huygens SFP Renderer generates realistic 3D scenes, based on a 3D microscopy image which is interpreted as a distribution of fluorescent material. The computational work is done by the Simulated Fluorescence Process (SFP) algorithm, simulating what happens when the material is excited and how the subsequently emitted light travels to the observer.

Image Description:
SFP rendering of a mouse blastocyst. Image shows an additional cutting plane, with a plane normal pointing roughly upward. Image courtesy of Dr. Marc Duque Ramirez, Dr. Ritsuya Niwayama (Hiiragi group), and Dr. Stefan Terjung (ALMF) from the EMBL in Heidelberg, Germany.

SFP Renderer Request Trail

The Maximum Intensity Projection (MIP) Renderer produces 3D to 2D projections of the highest intensities in an object. The MIP Renderer projects image voxels onto the screen by tracing rays from a given view-point through the data. For each ray the maximum encountered intensity is determined and displayed.

Image Description:
The MIP renderer in color mode depth-coding showing a Paramecium. Regions of the image close to the viewpoint appear blue whereas region far away appear red. Image courtesy of A. Aubusson-Fleury CNRS, Gif sur Yvette, Paris.

MIP Renderer Request Trail

The Maximum Intensity Projection (MIP) Renderer produces 3D to 2D projections of the highest intensities in an object. The Huygens Twin Slicer is a powerful visualization tool available in Huygens Essential, Huygens Professional and Huygens Localizer. The Twin Slicer allows you to synchronize and inspect views of two or three (different) images. Multiple channels are visible in one view. (Auto)contrast, gamma, and brightness can be quickly set, and different color schemes and LUTs can be selected. With a few simple mouse button clicks, you can measure intensities, sizes, distances, FWHM values, plot line profiles, and more.

Image Description:
Huygens Twin Slicer window in the Advanced mode showing the raw (left) and deconvolved (right) version of a Widefield image of the kinetochore complex. Automatic contrast was applied to both images. Data courtesy of Dr. Livio Kleij en Martijn Vroomans, Medical Oncology, UMC Utrecht, The Netherlands. Scale bar is one micron.

MIP Renderer Request Trail