Molecular Devices
The Molecular Devices ImageXPress Confocal Ht.ai is a spinning disk system that can image using 2 spinning disks. One containing an array of microlenses, which efficiently guides the laser beams into pinholes, and another disk type containing slits.
Pinhole disk
Like the Yokogawa Disks, the disk has a 1:10 ratio between physical pinhole spacing of 250 microns and pinhole radius of 25 microns (50 µm in diameter).
According to information we received, it has an internal magnification factor of 1.03. Follow the tables below to find the back projected pinhole radius and distance values.
If your microscope reports another internal magnification, then you should multiply these values by 1.03 and divide them by your specific internal magnification value to get the backprojected pinhole values that fit your microscope.
| Magnification | Back Projected Pinhole Radius | Back Projected Pinhole Distance |
|---|---|---|
| 100× | 243 nm | 2.43 µm |
| 60× | 404 nm | 4.04 µm |
| 40× | 607 nm | 6.06 µm |
| 20× | 1214 nm | 12.14 µm |
| 10× | 2427 nm | 24.27 µm |
Slit disk
As an additional imaging option, there also is a disk that contains slits instead of pinholes. These slits were reported to be 400 microns apart with slits of 50 microns wide. From the description, this seems to be more in line with the Olympus Disk Scanning Unit, however instead of a varying patterns of slits, it uses slits in the shape of outgoing spirals that scan across the sample. This changes the effective pinhole radius. An informative description on how to deconvolve these kind of images can be read on the page of the Olympus Disk Scanning Unit.