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Scientific Volume Imaging B.V.

Scientific Volume Imaging

Deconvolution - Visualization - Analysis


™Huygens Object Tracker

Study 3D motion of cells and smaller particles in time series

Object Analyzer

[Huygens-Colocalization-Analyzer|Colocalization Analyzer Object Tracker

Cluster Analyzer

Huygens Object Tracker tracks the movement of cells and particles in time series images. By manually selecting just a few objects and background regions, the machine learning algorithm in the Object Tracker is sufficiently trained to automatically detect new objects and to track these. Tracks can be filtered based on specific parameters, edited, and analyzed with the included Track Analyzer. Various properties of the measured tracks are presented in informative graphs and can be exported.

Image description FUCCI cells tracked for 50 seconds with Huygens Object Tracker. Courtesy of Dr. Richard Wubbolts, Center for Cell Imaging, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Image

Easy to use

Train the machine learning algorithm to identify all objects of interest by selecting a few examples.

Detection options

Object classification is based on various features, including brightness and size, and can be refined using additional filters.

Track Analyzer

Filter, edit, analyze and export the tracks with the intuitive Track Analyzer.

Main Features Object Tracker

Train machine learning algorithm with a few objects and background region.

Detect objects using brightness and other features.
Define thresholds on the number of objects, brightness, and width.
Use object geometry and orientation for connecting tracks.
Quick edit tracks (remove, break, connect).
Export tracks to Track Analyzer.
Save track detection settings to a template file to re-use.
Save tracks for future analysis in the Track Analyzer.

OT10

Main Features Track Analyzer

Visualize the MIP projection over time.
Find tracks that might need to be connected or split.
Find large objects that were incorrectly detected as two touching objects.
Add missed objects to tracks.
Analyze position; distance from track origin as histogram and time-plot, Mean Squared Displacement (MSD) and Diffusion Coefficient
Analyze speed; histogram, average speed, and x vs y velocity scatter plot.
Analyze flow; transport through a surface, defined using a line/poly-line in a Z-projection. Plot flux vs. time.
Analyze intensity of objects over time.
Analyze axial rotation and 3D angular speed of objects.
Export data to CSV file for any further analysis in e.g. Excel and Matlab.
Export graphs to EPS (Encapsulated PostScript) file.

track_analyzer_small.png

Use in research

D. Marks, N. Heinen, L. Bachmann et al. Amyloid precursor protein elevates fusion of promyelocytic leukemia nuclear bodies in human hippocampal areas with high plaque load. After Huygens Deconvolution, 3D motion of nuclear bodies was studied with the Huygens Object Tracker. Acta Neuropathol. Commun. 9, 66 (2021)

C. Eich, J. Arlt, C.S. Vink et al. In vivo single cell analysis reveals Gata2 dynamics in cells transitioning to hematopoietic fate. Huygens was used for deconvolution, drift correction, and cell tracking. J Exp Med 215(1), 233–248 (2018)

For more, see Scientific Publications

To track the movement of individual objects, it may be necessary to correct for sample drift with the Huygens Object Stabilizer.

Object stabilizer

More information

Live Cell imaging Support page Object Tracker webinar