File Open dialog: query data conversion
When you open a file saved in e.g. TIFF File Format it may happen that you get a dialog like this:
Input file is in 16 bit TIFF format: select target data type: To float To 16 bit signed To 8 bit unsigned
If you select a format other than Float Type, another dialog will pop up like follows:
Select conversion mode: Scale by 2 Clip values > 32767
If your microscope data was originally 8 or 12 bit saved in a 16 bit file this can be safely imported as "16 bit signed", with values larger than 32767 clipped. This is the recommended option in order to save memory.
Please see → Integer Type to understand what is happening here.