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Visitech Infinity


This is part of the Backprojected Pinhole Calculator.

Details


The microscope reports the diameter length (d) of the circular emission pinholes in the scanning array in microns (µm). Use this reported value in the form below.

The value will be averaged (RMS) with the fixed excitation pinhole of 50 µm.

The system magnification is reported to be 1.0. The simplified equation to calculate the Back Projected Pinhole Radius in nanometers is therefore r_b\approx 353.5 \sqrt{d^2 + 50^2} / m_{obj}.

Calculator

Detection pinhole diameter (microns)
Objective magnification



Pinhole distance


The pinhole distance in the array is always 250 µm, so the Back Projected Pinhole Distance is easy to calculate:

b.p. pinhole distance = 250 µm / objective_magnification

For a 100× lens, the backprojected pinhole distance is 250 µm / 100 = 2.5 µm. The following is a table with the most common magnification factors.

Magnification Back Projected Pinhole Distance
100 2.5 µm
60 4.17 µm
40 6.25 µm
20 12.5 µm
10 25.0 µm


Contact Information

Scientific Volume Imaging B.V.

Laapersveld 63
1213 VB Hilversum
The Netherlands


Phone: +31 (0)35 64216 26
Fax: +31 (0)35 683 7971
E-mail: info at svi.nl

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